11PI

Crystal structure of UFC1 variant Y42F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-fold modifier-conjugating enzyme 1	polymer	212	24731.0	1	UniProt (Q9Y3C8)	Homo sapiens (human)	Ufm1-conjugating enzyme 1
2	B (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
3	C (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	D (A)	water	water		18.0	200	Chemie (HOH)

Sequence modifications

A: 1 - 167 (UniProt: Q9Y3C8)

PDB	External Database	Details
Met -44	-	initiating methionine
Gly -43	-	expression tag
Ser -42	-	expression tag
Ser -41	-	expression tag
His -40	-	expression tag
His -39	-	expression tag
His -38	-	expression tag
His -37	-	expression tag
His -36	-	expression tag
His -35	-	expression tag
Ser -34	-	expression tag
Ser -33	-	expression tag
Gly -32	-	expression tag
Glu -31	-	expression tag
Thr -30	-	expression tag
Val -29	-	expression tag
Arg -28	-	expression tag
Phe -27	-	expression tag
Gln -26	-	expression tag
Gly -25	-	expression tag
Leu -24	-	expression tag
Asn -23	-	expression tag
Asp -22	-	expression tag
Ile -21	-	expression tag
Phe -20	-	expression tag
Glu -19	-	expression tag
Ala -18	-	expression tag
Gln -17	-	expression tag
Lys -16	-	expression tag
Ile -15	-	expression tag
Glu -14	-	expression tag
Trp -13	-	expression tag
His -12	-	expression tag
Glu -11	-	expression tag
Asp -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Phe 42	Tyr 42	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	24731.0
Non-Polymers*	Number of molecules	2
	Total formula weight	188.2
All*	Total formula weight	24919.2

*Water molecules are not included.