11MY
Cryo-EM structure of trimeric Nitrogenase MoFe protein from Methanosarcina acetivorans bound to the NifI inhibitor complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Nitrogenase protein alpha chain | polymer | 559 | 62884.3 | 2 | UniProt (Q8TJ90) Pfam (PF00148) | Methanosarcina acetivorans | |
| 2 | B, D (B, D) | Nitrogenase molybdenum-iron protein beta chain | polymer | 456 | 49478.6 | 2 | UniProt (Q8TJ89) Pfam (PF00148) | Methanosarcina acetivorans | Dinitrogenase |
| 3 | E (E) | P-II family nitrogen regulatory protein | polymer | 125 | 13980.3 | 1 | UniProt (Q8TJ91) Pfam (PF00543) | Methanosarcina acetivorans | |
| 4 | F, G (F, J) | P-II family nitrogen regulatory protein | polymer | 105 | 11874.0 | 2 | UniProt (Q8TJ92) Pfam (PF00543) | Methanosarcina acetivorans | |
| 5 | H (C) | 3-HYDROXY-3-CARBOXY-ADIPIC ACID | non-polymer | 206.2 | 1 | Chemie (HCA) PubChem (439459) | |||
| 6 | I (C) | iron-sulfur-molybdenum cluster with interstitial carbon | non-polymer | 787.5 | 1 | Chemie (ICS) | |||
| 7 | J (D) | FE(8)-S(7) CLUSTER | non-polymer | 671.2 | 1 | Chemie (CLF) | |||
| 8 | K, L, M (F, J) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 3 | Chemie (ADP) |
Sequence modifications
A, C: 1 - 528 (UniProt: Q8TJ90)
| PDB | External Database | Details |
|---|---|---|
| Met -30 | - | initiating methionine |
| Trp -29 | - | expression tag |
| Ser -28 | - | expression tag |
| His -27 | - | expression tag |
| Pro -26 | - | expression tag |
| Gln -25 | - | expression tag |
| Phe -24 | - | expression tag |
| Glu -23 | - | expression tag |
| Lys -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Gly -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Ser -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Trp -9 | - | expression tag |
| Ser -8 | - | expression tag |
| His -7 | - | expression tag |
| Pro -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Glu -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 262454.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2946.4 | |
| All* | Total formula weight | 265400.4 |
