11GQ

Cryo-EM structure of human CNNM4(E284A) tetramer with Magnesium and MgATP in outward-facing state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Metal transporter CNNM4	polymer	844	94082.6	4	UniProt (Q6P4Q7)	Homo sapiens (human)	Ancient conserved domain-containing protein 4,Cyclin-M4
2	E, F, G, H, I... (E, F, G, H, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB
3	EA, M, S, Y (D, A, B, C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (ATP)
4	AA, BA, CA, FA, GA... (C, D, A, B)	MAGNESIUM ION	non-polymer		24.3	16	Chemie (MG)
5	DA, JA, R, X (C, D, A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, B, C, D: 1 - 775 (UniProt: Q6P4Q7)

PDB	External Database	Details
Ala 284	Glu 284	engineered mutation
Val 776	-	expression tag
Asp 777	-	expression tag
Gly 778	-	expression tag
Ser 779	-	expression tag
Leu 780	-	expression tag
Glu 781	-	expression tag
Val 782	-	expression tag
Leu 783	-	expression tag
Phe 784	-	expression tag
Gln 785	-	expression tag
Gly 786	-	expression tag
Pro 787	-	expression tag
Ser 788	-	expression tag
Gly 789	-	expression tag
Gly 790	-	expression tag
Ser 791	-	expression tag
Arg 792	-	expression tag
Leu 793	-	expression tag
Glu 794	-	expression tag
Glu 795	-	expression tag
Glu 796	-	expression tag
Leu 797	-	expression tag
Arg 798	-	expression tag
Arg 799	-	expression tag
Arg 800	-	expression tag
Leu 801	-	expression tag
Thr 802	-	expression tag
Glu 803	-	expression tag
Gly 804	-	expression tag
Gly 805	-	expression tag
Ser 806	-	expression tag
Trp 807	-	expression tag
Ser 808	-	expression tag
His 809	-	expression tag
Pro 810	-	expression tag
Gln 811	-	expression tag
Phe 812	-	expression tag
Glu 813	-	expression tag
Lys 814	-	expression tag
Gly 815	-	expression tag
Gly 816	-	expression tag
Gly 817	-	expression tag
Ser 818	-	expression tag
Gly 819	-	expression tag
Gly 820	-	expression tag
Gly 821	-	expression tag
Ser 822	-	expression tag
Gly 823	-	expression tag
Gly 824	-	expression tag
Ser 825	-	expression tag
Ala 826	-	expression tag
Trp 827	-	expression tag
Ser 828	-	expression tag
His 829	-	expression tag
Pro 830	-	expression tag
Gln 831	-	expression tag
Phe 832	-	expression tag
Glu 833	-	expression tag
Lys 834	-	expression tag
Gly 835	-	expression tag
Ser 836	-	expression tag
His 837	-	expression tag
His 838	-	expression tag
His 839	-	expression tag
His 840	-	expression tag
His 841	-	expression tag
His 842	-	expression tag
His 843	-	expression tag
His 844	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	376330.5
Branched	Number of molecules	8
	Total formula weight	3395.2
Non-Polymers*	Number of molecules	24
	Total formula weight	3302.4
All*	Total formula weight	383028.1

*Water molecules are not included.