10ZB
Room-temperature X-ray structure of E53Q mutant of Thermus thermophilus serine hydroxymethyltransferase (TthSHMT) in complex with PLP-L-Ser external aldimine and tetrahydrofolate (THF)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Serine hydroxymethyltransferase | polymer | 405 | 44677.0 | 1 | UniProt (A0AAD1KUU5) | Thermus thermophilus | SHMT,Serine methylase |
| 2 | B (B) | Serine hydroxymethyltransferase | polymer | 405 | 44448.9 | 1 | UniProt (A0AAD1KUU5) | Thermus thermophilus | SHMT,Serine methylase |
| 3 | C (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) PubChem (175) | |||
| 4 | D, E, H (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 5 | F (B) | (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine | non-polymer | 334.2 | 1 | Chemie (KOU) | |||
| 6 | G (B) | (6S)-5,6,7,8-TETRAHYDROFOLATE | non-polymer | 445.4 | 1 | Chemie (THG) | |||
| 7 | I, J (A, B) | water | water | 18.0 | 403 | Chemie (HOH) |
Sequence modifications
A: 3 - 407 (UniProt: A0AAD1KUU5)
B: 1003 - 1407 (UniProt: A0AAD1KUU5)
| PDB | External Database | Details |
|---|---|---|
| Gln 53 | Glu 53 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gln 1053 | Glu 53 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 89125.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1126.9 | |
| All* | Total formula weight | 90252.8 |
