10TY
Tissue Non-specific Alkaline Phosphatase -S110A bound to PPi with ethylene glycol
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Alkaline phosphatase, tissue-nonspecific isozyme | polymer | 493 | 54634.3 | 4 | UniProt (P09242) Pfam (PF00245) | Mus musculus (house mouse) | AP-TNAP,TNAP,TNSALP,Alkaline phosphatase 2,Alkaline phosphatase liver/bone/kidney isozyme,Phosphoamidase,Phosphocreatine phosphatase |
| 2 | E, H (E, H) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 894.8 | 2 | In PDB GlyTouCan (G42466VF) | |||
| 3 | F (F) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 4 | G, J, K, M (G, J, K, M) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 5 | I (I) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 6 | L (L) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
| 7 | EA, MA, N, V (C, D, A, B) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 4 | Chemie (POP) | |||
| 8 | FA, NA, O, W (C, D, A, B) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) PubChem (32051) | |||
| 9 | GA, OA, P, X (C, D, A, B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 10 | HA, PA, Q, Y (C, D, A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 11 | AA, KA, QA, R (B, C, D, A) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) PubChem (923) | |||
| 12 | BA, CA, DA, LA, RA... (B, C, D, A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 9 | Chemie (NAG) PubChem (24139) | |||
| 13 | U (B) | alpha-D-mannopyranose | non-polymer | 180.2 | 1 | Chemie (MAN) PubChem (185698) | |||
| 14 | JA, Z (C, B) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) PubChem (1061) | |||
| 15 | IA (C) | CITRATE ANION | non-polymer | 189.1 | 1 | Chemie (FLC) PubChem (31348) | |||
| 16 | UA, VA, WA, XA (A, B, C, D) | water | water | 18.0 | 745 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 17 - 501 (UniProt: P09242)
| PDB | External Database | Details |
|---|---|---|
| Asp 15 | - | expression tag |
| Gly 16 | - | expression tag |
| Ala 110 | Ser 110 | engineered mutation |
| His 502 | - | expression tag |
| His 503 | - | expression tag |
| His 504 | - | expression tag |
| His 505 | - | expression tag |
| His 506 | - | expression tag |
| His 507 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 218537.0 | |
| Branched | Number of molecules | 9 |
| Total formula weight | 5733.3 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 3865.0 | |
| All* | Total formula weight | 228135.4 |
