10TW
Tissue Non-specific Alkaline Phosphatase -D370G bound to phosphocreatine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Alkaline phosphatase, tissue-nonspecific isozyme | polymer | 493 | 54592.2 | 4 | UniProt (P09242) Pfam (PF00245) | Mus musculus (house mouse) | AP-TNAP,TNAP,TNSALP,Alkaline phosphatase 2,Alkaline phosphatase liver/bone/kidney isozyme,Phosphoamidase,Phosphocreatine phosphatase |
| 2 | E, L (E, L) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 3 | F (F) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1057.0 | 1 | In PDB GlyTouCan (G82348BZ) | |||
| 4 | G (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 570.5 | 1 | In PDB GlyTouCan (G21290RB) | |||
| 5 | H, K (H, K) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 6 | I, M (I, M) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 894.8 | 2 | In PDB GlyTouCan (G42466VF) | |||
| 7 | J (J) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 732.7 | 1 | In PDB GlyTouCan (G32152BH) | |||
| 8 | N (N) | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G22573RC) | |||
| 9 | GA, O, QA, W (C, A, D, B) | PHOSPHATE ION | non-polymer | 95.0 | 4 | Chemie (PO4) PubChem (1061) | |||
| 10 | HA, P, RA, X (C, A, D, B) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) PubChem (32051) | |||
| 11 | IA, Q, SA, Y (C, A, D, B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 12 | JA, R, TA, Z (C, A, D, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 13 | AA, MA, S, VA (B, C, A, D) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) PubChem (923) | |||
| 14 | BA, CA, NA, OA, T... (B, C, A, D) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 9 | Chemie (NAG) PubChem (24139) | |||
| 15 | DA, EA, KA, PA, UA... (B, C, D, A) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) PubChem (753) | |||
| 16 | FA (C) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) PubChem (3777159) | |||
| 17 | LA (C) | CITRATE ANION | non-polymer | 189.1 | 1 | Chemie (FLC) PubChem (31348) | |||
| 18 | AB, BB, CB, ZA (B, C, D, A) | water | water | 18.0 | 1267 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 17 - 501 (UniProt: P09242)
| PDB | External Database | Details |
|---|---|---|
| Asp 15 | - | expression tag |
| Gly 16 | - | expression tag |
| Gly 370 | Asp 370 | engineered mutation |
| His 502 | - | expression tag |
| His 503 | - | expression tag |
| His 504 | - | expression tag |
| His 505 | - | expression tag |
| His 506 | - | expression tag |
| His 507 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 218368.9 | |
| Branched | Number of molecules | 10 |
| Total formula weight | 6920.4 | |
| Non-Polymers* | Number of molecules | 37 |
| Total formula weight | 3845.7 | |
| All* | Total formula weight | 229135.0 |
