10RR

Human RNase PNK bound to AMPPNP ligand + rCAA in PNK active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Ribosomal biogenesis protein LAS1L	polymer	777	87583.4	2	UniProt (Q9Y4W2)	Homo sapiens (human)	Endoribonuclease LAS1L,Protein LAS1 homolog
2	B, E (B, E)	Polynucleotide 5'-hydroxyl-kinase NOL9	polymer	605	68119.6	2	UniProt (Q5SY16)	Homo sapiens (human)	Nucleolar protein 9
3	C, F (C, F)	CAA RNA	polymer	3	918.6	2		Homo sapiens
4	G, I (B, E)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
5	H, J (B, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)

Sequence modifications

A, D: 1 - 734 (UniProt: Q9Y4W2)

PDB	External Database	Details
Met -42	-	expression tag
Ala -41	-	expression tag
Ser -40	-	expression tag
Trp -39	-	expression tag
Ser -38	-	expression tag
His -37	-	expression tag
Pro -36	-	expression tag
Gln -35	-	expression tag
Phe -34	-	expression tag
Glu -33	-	expression tag
Lys -32	-	expression tag
Gly -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Ala -28	-	expression tag
Arg -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Gly -11	-	expression tag
Phe -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag
Glu 155	Arg 155	engineered mutation
Ala 156	His 156	engineered mutation
Ala 160	His 160	engineered mutation

B, E: 104 - 702 (UniProt: Q5SY16)

PDB	External Database	Details
Gly 98	-	expression tag
Gly 99	-	expression tag
Gly 100	-	expression tag
Gly 101	-	expression tag
Gly 102	-	expression tag
Thr 103	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	313243.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.0
All*	Total formula weight	314304.2

*Water molecules are not included.