10QY

Cryo-EM structure of the Rad1-Rad10-Saw1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA repair protein RAD1	polymer	1129	129805.6	1	UniProt (P06777) Pfam (PF02732)	Saccharomyces cerevisiae (brewer's yeast)
2	B (B)	DNA repair protein RAD10	polymer	213	24614.6	1	UniProt (P06838) Pfam (PF03834)	Saccharomyces cerevisiae (brewer's yeast)
3	C (C)	Single-strand annealing weakened protein 1	polymer	264	30089.0	1	UniProt (P39735) Pfam (PF11561)	Saccharomyces cerevisiae (brewer's yeast)

Sequence modifications

A: 1 - 1100 (UniProt: P06777)

PDB	External Database	Details
Met -28	-	initiating methionine
Lys -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Gly -19	-	expression tag
Ala -18	-	expression tag
Ala -17	-	expression tag
Gly -16	-	expression tag
Thr -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Lys -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

B: 1 - 210 (UniProt: P06838)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag

C: 1 - 261 (UniProt: P39735)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	184509.2
All*	Total formula weight	184509.2

*Water molecules are not included.