10QI

Crystal Structure of Treponema denticola Sialidase (TDE_0471) D165A mutant bound to 3'-sialyllactose (only Neu5Ac visible)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	exo-alpha-sialidase	polymer	531	58744.7	1	UniProt (Q73QH2)	Treponema denticola
2	B (A)	N-acetyl-alpha-neuraminic acid	non-polymer		309.3	1	Chemie (SIA)
3	C, D, E, G (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	H, I, J, K, L... (A)	CADMIUM ION	non-polymer		112.4	6	Chemie (CD)
6	M (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	O (A)	water	water		18.0	495	Chemie (HOH)

Sequence modifications

A: 25 - 543 (UniProt: Q73QH2)

PDB	External Database	Details
Met 13	-	expression tag
Arg 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
Ala 165	Asp 165	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	58744.7
Non-Polymers*	Number of molecules	13
	Total formula weight	1481.2
All*	Total formula weight	60225.9

*Water molecules are not included.