10MK
SemiClosed Mtb-EC: Cryo-EM structure of Mtb RNAP elongation complex (substrate loading mimic) with a semiclosed active site (closed TL, open RH-FL)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 347 | 37745.3 | 2 | UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Mycobacterium tuberculosis | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (E) | DNA-directed RNA polymerase subunit omega | polymer | 110 | 11851.1 | 1 | UniProt (P9WGY5) Pfam (PF01192) | Mycobacterium tuberculosis | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 3 | D (P) | Template DNA | polymer | 54 | 16506.5 | 1 | Escherichia coli | ||
| 4 | E (R) | RNA | polymer | 20 | 6526.0 | 1 | Escherichia coli | ||
| 5 | F (C) | DNA-directed RNA polymerase subunit beta | polymer | 1178 | 130018.8 | 1 | UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Mycobacterium tuberculosis | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 6 | G (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1318 | 147097.1 | 1 | UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Mycobacterium tuberculosis | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 7 | H (Q) | Non-template DNA | polymer | 54 | 16589.6 | 1 | Escherichia coli | ||
| 8 | I (D) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 9 | J, K (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) | |||
| 10 | L, M (D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 11 | N, O, P, Q, R... (A, B, E, P, R...) | water | water | 18.0 | 904 | Chemie (HOH) |
Sequence modifications
D: 1 - 1316 (UniProt: P9WGY7)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 404079.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 702.6 | |
| All* | Total formula weight | 404782.4 |
