10MK

SemiClosed Mtb-EC: Cryo-EM structure of Mtb RNAP elongation complex (substrate loading mimic) with a semiclosed active site (closed TL, open RH-FL)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (P9WGZ1)	Mycobacterium tuberculosis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (E)	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	1	UniProt (P9WGY5)	Mycobacterium tuberculosis	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
3	D (P)	Template DNA	polymer	54	16506.5	1		Escherichia coli
4	E (R)	RNA	polymer	20	6526.0	1		Escherichia coli
5	F (C)	DNA-directed RNA polymerase subunit beta	polymer	1178	130018.8	1	UniProt (P9WGY9)	Mycobacterium tuberculosis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
6	G (D)	DNA-directed RNA polymerase subunit beta'	polymer	1318	147097.1	1	UniProt (P9WGY7)	Mycobacterium tuberculosis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
7	H (Q)	Non-template DNA	polymer	54	16589.6	1		Escherichia coli
8	I (D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
9	J, K (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
10	L, M (D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
11	N, O, P, Q, R... (A, B, E, P, R...)	water	water		18.0	904	Chemie (HOH)

Sequence modifications

D: 1 - 1316 (UniProt: P9WGY7)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	404079.8
Non-Polymers*	Number of molecules	5
	Total formula weight	702.6
All*	Total formula weight	404782.4

*Water molecules are not included.