10MB

Open1 Eco-ePEC: Cryo-EM structure of Eco RNAP his-elemental paused elongation complex with an open active site (open TL, SI3 and RH-FL)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	DNA	polymer	32	9706.3	1		Escherichia coli
2	B (K)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192)	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
3	C (R)	RNA (5'-R(P*GP*AP*UP*GP*UP*GP*UP*GP*CP*U)-3')	polymer	19	6047.6	1		Escherichia coli
4	D (A)	DNA (5'-D(*CP*GP*TP*CP*CP*TP*TP*TP*CP*GP*GP*AP*AP*GP*AP*GP*AP*TP*TP*CP*AP*GP*A)-3')	polymer	32	9881.4	1		Escherichia coli
5	E, F (G, H)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
6	G (I)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
7	H (J)	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
8	I (I)	CHAPSO	non-polymer		631.9	1	Chemie (1N7) PubChem (90479263)
9	J (J)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
10	K, L (J)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
11	M, N, O, P, Q... (B, K, R, A, G...)	water	water		18.0	674	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	415189.8
Non-Polymers*	Number of molecules	4
	Total formula weight	787.0
All*	Total formula weight	415976.8

*Water molecules are not included.