10MA
Closed Eco-ePEC: Cryo-EM structure of Eco RNAP his-elemental paused elongation complex with a closed active site (closed TL, SI3 and RH-FL)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | non-template DNA | polymer | 32 | 9881.4 | 1 | Escherichia coli | ||
| 2 | B (B) | template-DNA | polymer | 32 | 9706.3 | 1 | Escherichia coli | ||
| 3 | C, D (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 4 | E (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 5 | F (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli | RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta' |
| 6 | G (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 7 | H (R) | RNA | polymer | 19 | 6047.6 | 1 | Escherichia coli | ||
| 8 | I, J (I) | CHAPSO | non-polymer | 631.9 | 2 | Chemie (1N7) PubChem (90479263) | |||
| 9 | K (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 10 | L, M (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) | |||
| 11 | N, O, P, Q, R... (A, B, G, H, I...) | water | water | 18.0 | 611 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 415189.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1418.9 | |
| All* | Total formula weight | 416608.7 |
