10JU

Crystal Structure of serine/threonine-protein kinase (AEK1) T376D, S395D Mutant from Trypanosoma brucei (AMP-PNP)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Serine/threonine-protein kinase, putative	polymer	362	41175.8	1	UniProt (Q582V7)	Trypanosoma brucei brucei TREU927
2	B (B)	Serine/threonine-protein kinase, putative	polymer	362	41095.8	1	UniProt (Q582V7)	Trypanosoma brucei brucei TREU927
3	C, D, E, F, G... (A, B)	SULFATE ION	non-polymer		96.1	11	Chemie (SO4)
4	J (B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
5	O, P (A, B)	water	water		18.0	88	Chemie (HOH)

Sequence modifications

A: 54 - 406 (UniProt: Q582V7)

PDB	External Database	Details
Met 53	-	initiating methionine
Asp 376	Thr 376	engineered mutation
Asp 395	Ser 395	engineered mutation
Leu 407	-	expression tag
Glu 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag

B: 54 - 406 (UniProt: Q582V7)

PDB	External Database	Details
Met 53	-	initiating methionine
Asp 376	Thr 376	engineered mutation
Asp 395	Ser 395	engineered mutation
Leu 407	-	expression tag
Glu 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	82271.6
Non-Polymers*	Number of molecules	12
	Total formula weight	1562.9
All*	Total formula weight	83834.5

*Water molecules are not included.