10GD

The eukaryotic translation initiation factor 2B from Homo sapiens with a L179V mutation in the delta subunit bound to ISRIB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, B)	Translation initiation factor eIF-2B subunit epsilon	polymer	721	80452.6	2	UniProt (Q13144) Pfam (PF02020)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit epsilon
2	B, G (C, D)	Translation initiation factor eIF-2B subunit beta	polymer	368	41008.6	2	UniProt (P49770) Pfam (PF01008)	Homo sapiens (human)	S20I15,S20III15,eIF-2B GDP-GTP exchange factor subunit beta
3	C, H (E, F)	Translation initiation factor eIF2B subunit delta	polymer	523	57626.1	2	UniProt (Q9UI10) Pfam (PF01008)	Homo sapiens (human)	eIF2B GDP-GTP exchange factor subunit delta
4	D, I (G, H)	Translation initiation factor eIF2B subunit alpha	polymer	322	35767.3	2	UniProt (Q14232) Pfam (PF01008)	Homo sapiens (human)	eIF2B GDP-GTP exchange factor subunit alpha
5	E, J (I, J)	Translation initiation factor eIF-2B subunit gamma	polymer	452	50304.2	2	UniProt (Q9NR50) Pfam (PF00483) Pfam (PF25084)	Homo sapiens (human)	eIF-2B GDP-GTP exchange factor subunit gamma
6	K, N, O, Q (C, E, D, F)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4) PubChem (1061)
7	L, S (E, F)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
8	M, R (E, F)	IMIDAZOLE	non-polymer		69.1	2	Chemie (IMD)
9	P (D)	2-(4-chloranylphenoxy)-~{N}-[4-[2-(4-chloranylphenoxy)ethanoylamino]cyclohexyl]ethanamide	non-polymer		451.3	1	Chemie (C7B)

Sequence modifications

A, B: 1 - 721 (UniProt: Q13144)

PDB	External Database	Details
Val 587	Ile 587	conflict

C, D: 2 - 351 (UniProt: P49770)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Ser 1	-	expression tag

E, F: 1 - 523 (UniProt: Q9UI10)

PDB	External Database	Details
Val 179	Leu 179	engineered mutation

G, H: 1 - 305 (UniProt: Q14232)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	530317.7
Non-Polymers*	Number of molecules	9
	Total formula weight	1100.2
All*	Total formula weight	531417.9

*Water molecules are not included.