9Z67

Cryo-EM structure of human Wntless-Wnt5a 2:2 complex - Composite map

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001837	biological_process	epithelial to mesenchymal transition
A	0001938	biological_process	positive regulation of endothelial cell proliferation
A	0002088	biological_process	lens development in camera-type eye
A	0002720	biological_process	positive regulation of cytokine production involved in immune response
A	0003138	biological_process	primary heart field specification
A	0003139	biological_process	secondary heart field specification
A	0003281	biological_process	ventricular septum development
A	0003283	biological_process	atrial septum development
A	0003408	biological_process	optic cup formation involved in camera-type eye development
A	0005102	molecular_function	signaling receptor binding
A	0005109	molecular_function	frizzled binding
A	0005115	molecular_function	receptor tyrosine kinase-like orphan receptor binding
A	0005125	molecular_function	cytokine activity
A	0005515	molecular_function	protein binding
A	0005543	molecular_function	phospholipid binding
A	0005576	cellular_component	extracellular region
A	0005788	cellular_component	endoplasmic reticulum lumen
A	0005796	cellular_component	Golgi lumen
A	0005886	cellular_component	plasma membrane
A	0006954	biological_process	inflammatory response
A	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
A	0007223	biological_process	Wnt signaling pathway, calcium modulating pathway
A	0007409	biological_process	axonogenesis
A	0007411	biological_process	axon guidance
A	0008104	biological_process	intracellular protein localization
A	0008584	biological_process	male gonad development
A	0008595	biological_process	anterior/posterior axis specification, embryo
A	0009986	cellular_component	cell surface
A	0010595	biological_process	positive regulation of endothelial cell migration
A	0010628	biological_process	positive regulation of gene expression
A	0010742	biological_process	macrophage derived foam cell differentiation
A	0010820	biological_process	positive regulation of T cell chemotaxis
A	0010976	biological_process	positive regulation of neuron projection development
A	0016055	biological_process	Wnt signaling pathway
A	0019904	molecular_function	protein domain specific binding
A	0021891	biological_process	olfactory bulb interneuron development
A	0022008	biological_process	neurogenesis
A	0030182	biological_process	neuron differentiation
A	0030216	biological_process	keratinocyte differentiation
A	0030666	cellular_component	endocytic vesicle membrane
A	0030669	cellular_component	clathrin-coated endocytic vesicle membrane
A	0030901	biological_process	midbrain development
A	0031012	cellular_component	extracellular matrix
A	0032722	biological_process	positive regulation of chemokine production
A	0032729	biological_process	positive regulation of type II interferon production
A	0032731	biological_process	positive regulation of interleukin-1 beta production
A	0032755	biological_process	positive regulation of interleukin-6 production
A	0032757	biological_process	positive regulation of interleukin-8 production
A	0032760	biological_process	positive regulation of tumor necrosis factor production
A	0032880	biological_process	regulation of protein localization
A	0035022	biological_process	positive regulation of Rac protein signal transduction
A	0035567	biological_process	non-canonical Wnt signaling pathway
A	0036517	biological_process	chemoattraction of serotonergic neuron axon
A	0036518	biological_process	chemorepulsion of dopaminergic neuron axon
A	0042060	biological_process	wound healing
A	0043032	biological_process	positive regulation of macrophage activation
A	0043066	biological_process	negative regulation of apoptotic process
A	0043122	biological_process	regulation of canonical NF-kappaB signal transduction
A	0043410	biological_process	positive regulation of MAPK cascade
A	0045165	biological_process	cell fate commitment
A	0045599	biological_process	negative regulation of fat cell differentiation
A	0045732	biological_process	positive regulation of protein catabolic process
A	0045766	biological_process	positive regulation of angiogenesis
A	0045778	biological_process	positive regulation of ossification
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0045893	biological_process	positive regulation of DNA-templated transcription
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0046330	biological_process	positive regulation of JNK cascade
A	0048018	molecular_function	receptor ligand activity
A	0048022	biological_process	negative regulation of melanin biosynthetic process
A	0048146	biological_process	positive regulation of fibroblast proliferation
A	0048706	biological_process	embryonic skeletal system development
A	0048806	biological_process	genitalia development
A	0048812	biological_process	neuron projection morphogenesis
A	0050727	biological_process	regulation of inflammatory response
A	0050729	biological_process	positive regulation of inflammatory response
A	0050807	biological_process	regulation of synapse organization
A	0051057	biological_process	positive regulation of small GTPase mediated signal transduction
A	0051885	biological_process	positive regulation of timing of anagen
A	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
A	0051964	biological_process	negative regulation of synapse assembly
A	0060070	biological_process	canonical Wnt signaling pathway
A	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
A	0060324	biological_process	face development
A	0060340	biological_process	positive regulation of type I interferon-mediated signaling pathway
A	0060760	biological_process	positive regulation of response to cytokine stimulus
A	0060907	biological_process	positive regulation of macrophage cytokine production
A	0062009	biological_process	secondary palate development
A	0070062	cellular_component	extracellular exosome
A	0071219	biological_process	cellular response to molecule of bacterial origin
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0071277	biological_process	cellular response to calcium ion
A	0071300	biological_process	cellular response to retinoic acid
A	0071346	biological_process	cellular response to type II interferon
A	0071425	biological_process	hematopoietic stem cell proliferation
A	0071542	biological_process	dopaminergic neuron differentiation
A	0071560	biological_process	cellular response to transforming growth factor beta stimulus
A	0072201	biological_process	negative regulation of mesenchymal cell proliferation
A	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
A	0097325	biological_process	melanocyte proliferation
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099054	biological_process	presynapse assembly
A	0099068	biological_process	postsynapse assembly
A	0099175	biological_process	regulation of postsynapse organization
A	0099566	biological_process	regulation of postsynaptic cytosolic calcium ion concentration
A	0150012	biological_process	positive regulation of neuron projection arborization
A	1900020	biological_process	positive regulation of protein kinase C activity
A	1902379	molecular_function	chemoattractant activity involved in axon guidance
A	1902474	biological_process	positive regulation of protein localization to synapse
A	1904861	biological_process	excitatory synapse assembly
A	1904862	biological_process	inhibitory synapse assembly
A	1904934	biological_process	negative regulation of cell proliferation in midbrain
A	1904948	biological_process	midbrain dopaminergic neuron differentiation
A	2000052	biological_process	positive regulation of non-canonical Wnt signaling pathway
A	2001235	biological_process	positive regulation of apoptotic signaling pathway
B	0000139	cellular_component	Golgi membrane
B	0001707	biological_process	mesoderm formation
B	0005515	molecular_function	protein binding
B	0005769	cellular_component	early endosome
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005794	cellular_component	Golgi apparatus
B	0005802	cellular_component	trans-Golgi network
B	0005886	cellular_component	plasma membrane
B	0006886	biological_process	intracellular protein transport
B	0009948	biological_process	anterior/posterior axis specification
B	0012505	cellular_component	endomembrane system
B	0016055	biological_process	Wnt signaling pathway
B	0017147	molecular_function	Wnt-protein binding
B	0030177	biological_process	positive regulation of Wnt signaling pathway
B	0030659	cellular_component	cytoplasmic vesicle membrane
B	0030666	cellular_component	endocytic vesicle membrane
B	0030901	biological_process	midbrain development
B	0030902	biological_process	hindbrain development
B	0031017	biological_process	exocrine pancreas development
B	0031410	cellular_component	cytoplasmic vesicle
B	0031901	cellular_component	early endosome membrane
B	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
B	0061355	biological_process	Wnt protein secretion
B	0061357	biological_process	positive regulation of Wnt protein secretion
B	0070062	cellular_component	extracellular exosome
B	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
C	0001837	biological_process	epithelial to mesenchymal transition
C	0001938	biological_process	positive regulation of endothelial cell proliferation
C	0002088	biological_process	lens development in camera-type eye
C	0002720	biological_process	positive regulation of cytokine production involved in immune response
C	0003138	biological_process	primary heart field specification
C	0003139	biological_process	secondary heart field specification
C	0003281	biological_process	ventricular septum development
C	0003283	biological_process	atrial septum development
C	0003408	biological_process	optic cup formation involved in camera-type eye development
C	0005102	molecular_function	signaling receptor binding
C	0005109	molecular_function	frizzled binding
C	0005115	molecular_function	receptor tyrosine kinase-like orphan receptor binding
C	0005125	molecular_function	cytokine activity
C	0005515	molecular_function	protein binding
C	0005543	molecular_function	phospholipid binding
C	0005576	cellular_component	extracellular region
C	0005788	cellular_component	endoplasmic reticulum lumen
C	0005796	cellular_component	Golgi lumen
C	0005886	cellular_component	plasma membrane
C	0006954	biological_process	inflammatory response
C	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
C	0007223	biological_process	Wnt signaling pathway, calcium modulating pathway
C	0007409	biological_process	axonogenesis
C	0007411	biological_process	axon guidance
C	0008104	biological_process	intracellular protein localization
C	0008584	biological_process	male gonad development
C	0008595	biological_process	anterior/posterior axis specification, embryo
C	0009986	cellular_component	cell surface
C	0010595	biological_process	positive regulation of endothelial cell migration
C	0010628	biological_process	positive regulation of gene expression
C	0010742	biological_process	macrophage derived foam cell differentiation
C	0010820	biological_process	positive regulation of T cell chemotaxis
C	0010976	biological_process	positive regulation of neuron projection development
C	0016055	biological_process	Wnt signaling pathway
C	0019904	molecular_function	protein domain specific binding
C	0021891	biological_process	olfactory bulb interneuron development
C	0022008	biological_process	neurogenesis
C	0030182	biological_process	neuron differentiation
C	0030216	biological_process	keratinocyte differentiation
C	0030666	cellular_component	endocytic vesicle membrane
C	0030669	cellular_component	clathrin-coated endocytic vesicle membrane
C	0030901	biological_process	midbrain development
C	0031012	cellular_component	extracellular matrix
C	0032722	biological_process	positive regulation of chemokine production
C	0032729	biological_process	positive regulation of type II interferon production
C	0032731	biological_process	positive regulation of interleukin-1 beta production
C	0032755	biological_process	positive regulation of interleukin-6 production
C	0032757	biological_process	positive regulation of interleukin-8 production
C	0032760	biological_process	positive regulation of tumor necrosis factor production
C	0032880	biological_process	regulation of protein localization
C	0035022	biological_process	positive regulation of Rac protein signal transduction
C	0035567	biological_process	non-canonical Wnt signaling pathway
C	0036517	biological_process	chemoattraction of serotonergic neuron axon
C	0036518	biological_process	chemorepulsion of dopaminergic neuron axon
C	0042060	biological_process	wound healing
C	0043032	biological_process	positive regulation of macrophage activation
C	0043066	biological_process	negative regulation of apoptotic process
C	0043122	biological_process	regulation of canonical NF-kappaB signal transduction
C	0043410	biological_process	positive regulation of MAPK cascade
C	0045165	biological_process	cell fate commitment
C	0045599	biological_process	negative regulation of fat cell differentiation
C	0045732	biological_process	positive regulation of protein catabolic process
C	0045766	biological_process	positive regulation of angiogenesis
C	0045778	biological_process	positive regulation of ossification
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0045944	biological_process	positive regulation of transcription by RNA polymerase II
C	0046330	biological_process	positive regulation of JNK cascade
C	0048018	molecular_function	receptor ligand activity
C	0048022	biological_process	negative regulation of melanin biosynthetic process
C	0048146	biological_process	positive regulation of fibroblast proliferation
C	0048706	biological_process	embryonic skeletal system development
C	0048806	biological_process	genitalia development
C	0048812	biological_process	neuron projection morphogenesis
C	0050727	biological_process	regulation of inflammatory response
C	0050729	biological_process	positive regulation of inflammatory response
C	0050807	biological_process	regulation of synapse organization
C	0051057	biological_process	positive regulation of small GTPase mediated signal transduction
C	0051885	biological_process	positive regulation of timing of anagen
C	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
C	0051964	biological_process	negative regulation of synapse assembly
C	0060070	biological_process	canonical Wnt signaling pathway
C	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
C	0060324	biological_process	face development
C	0060340	biological_process	positive regulation of type I interferon-mediated signaling pathway
C	0060760	biological_process	positive regulation of response to cytokine stimulus
C	0060907	biological_process	positive regulation of macrophage cytokine production
C	0062009	biological_process	secondary palate development
C	0070062	cellular_component	extracellular exosome
C	0071219	biological_process	cellular response to molecule of bacterial origin
C	0071222	biological_process	cellular response to lipopolysaccharide
C	0071277	biological_process	cellular response to calcium ion
C	0071300	biological_process	cellular response to retinoic acid
C	0071346	biological_process	cellular response to type II interferon
C	0071425	biological_process	hematopoietic stem cell proliferation
C	0071542	biological_process	dopaminergic neuron differentiation
C	0071560	biological_process	cellular response to transforming growth factor beta stimulus
C	0072201	biological_process	negative regulation of mesenchymal cell proliferation
C	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
C	0097325	biological_process	melanocyte proliferation
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0099054	biological_process	presynapse assembly
C	0099068	biological_process	postsynapse assembly
C	0099175	biological_process	regulation of postsynapse organization
C	0099566	biological_process	regulation of postsynaptic cytosolic calcium ion concentration
C	0150012	biological_process	positive regulation of neuron projection arborization
C	1900020	biological_process	positive regulation of protein kinase C activity
C	1902379	molecular_function	chemoattractant activity involved in axon guidance
C	1902474	biological_process	positive regulation of protein localization to synapse
C	1904861	biological_process	excitatory synapse assembly
C	1904862	biological_process	inhibitory synapse assembly
C	1904934	biological_process	negative regulation of cell proliferation in midbrain
C	1904948	biological_process	midbrain dopaminergic neuron differentiation
C	2000052	biological_process	positive regulation of non-canonical Wnt signaling pathway
C	2001235	biological_process	positive regulation of apoptotic signaling pathway
D	0000139	cellular_component	Golgi membrane
D	0001707	biological_process	mesoderm formation
D	0005515	molecular_function	protein binding
D	0005769	cellular_component	early endosome
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005794	cellular_component	Golgi apparatus
D	0005802	cellular_component	trans-Golgi network
D	0005886	cellular_component	plasma membrane
D	0006886	biological_process	intracellular protein transport
D	0009948	biological_process	anterior/posterior axis specification
D	0012505	cellular_component	endomembrane system
D	0016055	biological_process	Wnt signaling pathway
D	0017147	molecular_function	Wnt-protein binding
D	0030177	biological_process	positive regulation of Wnt signaling pathway
D	0030659	cellular_component	cytoplasmic vesicle membrane
D	0030666	cellular_component	endocytic vesicle membrane
D	0030901	biological_process	midbrain development
D	0030902	biological_process	hindbrain development
D	0031017	biological_process	exocrine pancreas development
D	0031410	cellular_component	cytoplasmic vesicle
D	0031901	cellular_component	early endosome membrane
D	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
D	0061355	biological_process	Wnt protein secretion
D	0061357	biological_process	positive regulation of Wnt protein secretion
D	0070062	cellular_component	extracellular exosome
D	0090263	biological_process	positive regulation of canonical Wnt signaling pathway

Functional Information from PROSITE/UniProt

site_id	PS00246
Number of Residues	10
Details	WNT1 Wnt-1 family signature. CKCHGVSGsC

Chain	Residue	Details
A	CYS238-CYS247

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Lipidation: {"description":"O-palmitoleoyl serine; by PORCN","evidences":[{"source":"UniProtKB","id":"P56704","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	506
Details	Topological domain: {"description":"Lumenal","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	98
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"PubMed","id":"20652957","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	262
Details	Region: {"description":"Interaction with Wnt proteins","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---