9W3G

Cryo-EM structure of E. coli RNA polymerase in complex with VP1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed RNA polymerase activity
A	0005737	cellular_component	cytoplasm
A	0006351	biological_process	DNA-templated transcription
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed RNA polymerase activity
B	0005737	cellular_component	cytoplasm
B	0006351	biological_process	DNA-templated transcription
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed RNA polymerase activity
C	0006351	biological_process	DNA-templated transcription
D	0000287	molecular_function	magnesium ion binding
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed RNA polymerase activity
D	0005829	cellular_component	cytosol
D	0006351	biological_process	DNA-templated transcription
D	0008270	molecular_function	zinc ion binding
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed RNA polymerase activity
E	0006351	biological_process	DNA-templated transcription
F	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
F	0003677	molecular_function	DNA binding
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006351	biological_process	DNA-templated transcription
F	0006352	biological_process	DNA-templated transcription initiation
F	0009408	biological_process	response to heat
F	0010468	biological_process	regulation of gene expression
F	0016987	molecular_function	sigma factor activity
F	0045892	biological_process	negative regulation of DNA-templated transcription
F	1903865	cellular_component	sigma factor antagonist complex
F	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PROSITE/UniProt

site_id	PS00715
Number of Residues	14
Details	SIGMA70_1 Sigma-70 factors family signature 1. DLIQeGnIGLMkAV

Chain	Residue	Details
F	ASP403-VAL416

---

site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TleEVGkqfdVTrerIrQIEakaLrkL

Chain	Residue	Details
F	THR572-LEU598

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI11
Number of Residues	19
Details	DNA binding: {"description":"H-T-H motif","evidences":[{"source":"HAMAP-Rule","id":"MF_00963","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI12
Number of Residues	70
Details	Region: {"description":"Sigma-70 factor domain-2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	76
Details	Region: {"description":"Sigma-70 factor domain-3"}

Chain

Residue

Details

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site_id	SWS_FT_FI14
Number of Residues	53
Details	Region: {"description":"Sigma-70 factor domain-4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	15
Details	Region: {"description":"Interaction with anti-sigma factors"}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	3
Details	Motif: {"description":"Interaction with polymerase core subunit RpoC"}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	1
Details	Site: {"description":"Interaction with anti-sigma factors"}

Chain

Residue

Details

---