9S3K
Crystal structure of ancestral (Methanocaldococcaceae/A34) Malate dehydrogenase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006089 | biological_process | lactate metabolic process |
| A | 0030060 | molecular_function | L-malate dehydrogenase (NAD+) activity |
| A | 0042867 | biological_process | pyruvate catabolic process |
| A | 0046554 | molecular_function | L-malate dehydrogenase (NADP+) activity |
| A | 0102443 | molecular_function | L-2-hydroxycarboxylate dehydrogenase (NAD+) activity |
| B | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006089 | biological_process | lactate metabolic process |
| B | 0030060 | molecular_function | L-malate dehydrogenase (NAD+) activity |
| B | 0042867 | biological_process | pyruvate catabolic process |
| B | 0046554 | molecular_function | L-malate dehydrogenase (NADP+) activity |
| B | 0102443 | molecular_function | L-2-hydroxycarboxylate dehydrogenase (NAD+) activity |
| C | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006089 | biological_process | lactate metabolic process |
| C | 0030060 | molecular_function | L-malate dehydrogenase (NAD+) activity |
| C | 0042867 | biological_process | pyruvate catabolic process |
| C | 0046554 | molecular_function | L-malate dehydrogenase (NADP+) activity |
| C | 0102443 | molecular_function | L-2-hydroxycarboxylate dehydrogenase (NAD+) activity |
| D | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006089 | biological_process | lactate metabolic process |
| D | 0030060 | molecular_function | L-malate dehydrogenase (NAD+) activity |
| D | 0042867 | biological_process | pyruvate catabolic process |
| D | 0046554 | molecular_function | L-malate dehydrogenase (NADP+) activity |
| D | 0102443 | molecular_function | L-2-hydroxycarboxylate dehydrogenase (NAD+) activity |
Functional Information from PROSITE/UniProt
| site_id | PS00064 |
| Number of Residues | 7 |
| Details | L_LDH L-lactate dehydrogenase active site. IGEHGDT |
| Chain | Residue | Details |
| A | ILE175-THR181 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"UniProtKB","id":"P61889","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 20 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"11292347","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1HYG","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"1HYG","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 15 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P61889","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Site: {"description":"Discrimination site allowing the use of NADPH","evidences":[{"source":"PubMed","id":"10998181","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | Site: {"description":"Discrimination site conferring specificity for malate over lactate","evidences":[{"source":"PubMed","id":"10998181","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
