9RGR

Crystal Structure of Rattus norvegicus Enoyl-CoA Hydratase in complex with 3S-hydroxybutanoyl-CoA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004165	molecular_function	delta(3)-delta(2)-enoyl-CoA isomerase activity
A	0004300	molecular_function	enoyl-CoA hydratase activity
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0006574	biological_process	L-valine catabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
A	0019477	biological_process	L-lysine catabolic process
A	0043956	molecular_function	3-hydroxypropionyl-CoA dehydratase activity
A	0120092	molecular_function	(2E)-butenoyl-CoA hydratase activity
B	0004165	molecular_function	delta(3)-delta(2)-enoyl-CoA isomerase activity
B	0004300	molecular_function	enoyl-CoA hydratase activity
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0006574	biological_process	L-valine catabolic process
B	0006635	biological_process	fatty acid beta-oxidation
B	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
B	0019477	biological_process	L-lysine catabolic process
B	0043956	molecular_function	3-hydroxypropionyl-CoA dehydratase activity
B	0120092	molecular_function	(2E)-butenoyl-CoA hydratase activity
C	0004165	molecular_function	delta(3)-delta(2)-enoyl-CoA isomerase activity
C	0004300	molecular_function	enoyl-CoA hydratase activity
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0006574	biological_process	L-valine catabolic process
C	0006635	biological_process	fatty acid beta-oxidation
C	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
C	0019477	biological_process	L-lysine catabolic process
C	0043956	molecular_function	3-hydroxypropionyl-CoA dehydratase activity
C	0120092	molecular_function	(2E)-butenoyl-CoA hydratase activity
D	0004165	molecular_function	delta(3)-delta(2)-enoyl-CoA isomerase activity
D	0004300	molecular_function	enoyl-CoA hydratase activity
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0006574	biological_process	L-valine catabolic process
D	0006635	biological_process	fatty acid beta-oxidation
D	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
D	0019477	biological_process	L-lysine catabolic process
D	0043956	molecular_function	3-hydroxypropionyl-CoA dehydratase activity
D	0120092	molecular_function	(2E)-butenoyl-CoA hydratase activity
E	0004165	molecular_function	delta(3)-delta(2)-enoyl-CoA isomerase activity
E	0004300	molecular_function	enoyl-CoA hydratase activity
E	0005739	cellular_component	mitochondrion
E	0005759	cellular_component	mitochondrial matrix
E	0006574	biological_process	L-valine catabolic process
E	0006635	biological_process	fatty acid beta-oxidation
E	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
E	0019477	biological_process	L-lysine catabolic process
E	0043956	molecular_function	3-hydroxypropionyl-CoA dehydratase activity
E	0120092	molecular_function	(2E)-butenoyl-CoA hydratase activity
F	0004165	molecular_function	delta(3)-delta(2)-enoyl-CoA isomerase activity
F	0004300	molecular_function	enoyl-CoA hydratase activity
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0006574	biological_process	L-valine catabolic process
F	0006635	biological_process	fatty acid beta-oxidation
F	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
F	0019477	biological_process	L-lysine catabolic process
F	0043956	molecular_function	3-hydroxypropionyl-CoA dehydratase activity
F	0120092	molecular_function	(2E)-butenoyl-CoA hydratase activity

Functional Information from PROSITE/UniProt

site_id	PS00166
Number of Residues	21
Details	ENOYL_COA_HYDRATASE Enoyl-CoA hydratase/isomerase signature. IAaVNGyalGGGcelaMmCDI

Chain	Residue	Details
B	ILE131-ILE151
A	ILE131-ILE151

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	Binding site: {}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	6
Details	Site: {"description":"Important for catalytic activity"}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	8
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"Q8BH95","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	6
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q8BH95","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	6
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q8BH95","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 315

Chain	Residue	Details
B	ALA98	activator, electrostatic stabiliser
B	GLY141	activator, electrostatic stabiliser
B	GLU144	hydrogen bond acceptor, increase basicity
B	GLU164	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 315

Chain	Residue	Details
C	ALA98	activator, electrostatic stabiliser
C	GLY141	activator, electrostatic stabiliser
C	GLU144	hydrogen bond acceptor, increase basicity
C	GLU164	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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site_id	MCSA3
Number of Residues	4
Details	M-CSA 315

Chain	Residue	Details
D	ALA98	activator, electrostatic stabiliser
D	GLY141	activator, electrostatic stabiliser
D	GLU144	hydrogen bond acceptor, increase basicity
D	GLU164	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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site_id	MCSA4
Number of Residues	4
Details	M-CSA 315

Chain	Residue	Details
F	ALA98	activator, electrostatic stabiliser
F	GLY141	activator, electrostatic stabiliser
F	GLU144	hydrogen bond acceptor, increase basicity
F	GLU164	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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