9R3D

Cryo-EM structure of the human pre-Bact-OTS complex (whole map)

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000244	biological_process	spliceosomal tri-snRNP complex assembly
A	0000375	biological_process	RNA splicing, via transesterification reactions
A	0000387	biological_process	spliceosomal snRNP assembly
A	0000388	biological_process	spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005681	cellular_component	spliceosomal complex
A	0005682	cellular_component	U5 snRNP
A	0006397	biological_process	mRNA processing
A	0008233	molecular_function	peptidase activity
A	0008380	biological_process	RNA splicing
A	0016020	cellular_component	membrane
A	0016607	cellular_component	nuclear speck
A	0030532	cellular_component	small nuclear ribonucleoprotein complex
A	0045292	biological_process	mRNA cis splicing, via spliceosome
A	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
A	0048025	biological_process	negative regulation of mRNA splicing, via spliceosome
A	0070530	molecular_function	K63-linked polyubiquitin modification-dependent protein binding
A	0071005	cellular_component	U2-type precatalytic spliceosome
A	0071006	cellular_component	U2-type catalytic step 1 spliceosome
A	0071007	cellular_component	U2-type catalytic step 2 spliceosome
A	0071009	cellular_component	U4atac/U6atac x U5 tri-snRNP complex
A	0071011	cellular_component	precatalytic spliceosome
A	0071013	cellular_component	catalytic step 2 spliceosome
A	0071014	cellular_component	post-mRNA release spliceosomal complex
A	0071016	cellular_component	U12-type precatalytic spliceosome
A	0071018	cellular_component	U12-type catalytic step 2 spliceosome
A	0071020	cellular_component	post-spliceosomal complex
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0071356	biological_process	cellular response to tumor necrosis factor
A	0140492	molecular_function	metal-dependent deubiquitinase activity
B	0000244	biological_process	spliceosomal tri-snRNP complex assembly
B	0000354	biological_process	cis assembly of pre-catalytic spliceosome
B	0000387	biological_process	spliceosomal snRNP assembly
B	0000388	biological_process	spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0001649	biological_process	osteoblast differentiation
B	0003723	molecular_function	RNA binding
B	0003724	molecular_function	RNA helicase activity
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005681	cellular_component	spliceosomal complex
B	0005682	cellular_component	U5 snRNP
B	0016020	cellular_component	membrane
B	0016887	molecular_function	ATP hydrolysis activity
B	0030532	cellular_component	small nuclear ribonucleoprotein complex
B	0042802	molecular_function	identical protein binding
B	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
B	0048025	biological_process	negative regulation of mRNA splicing, via spliceosome
B	0071005	cellular_component	U2-type precatalytic spliceosome
B	0071006	cellular_component	U2-type catalytic step 1 spliceosome
B	0071007	cellular_component	U2-type catalytic step 2 spliceosome
B	0071009	cellular_component	U4atac/U6atac x U5 tri-snRNP complex
B	0071011	cellular_component	precatalytic spliceosome
B	0071013	cellular_component	catalytic step 2 spliceosome
B	0071016	cellular_component	U12-type precatalytic spliceosome
B	0071018	cellular_component	U12-type catalytic step 2 spliceosome
B	0071020	cellular_component	post-spliceosomal complex
C	0000244	biological_process	spliceosomal tri-snRNP complex assembly
C	0000387	biological_process	spliceosomal snRNP assembly
C	0000388	biological_process	spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
C	0000398	biological_process	mRNA splicing, via spliceosome
C	0003723	molecular_function	RNA binding
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005681	cellular_component	spliceosomal complex
C	0005682	cellular_component	U5 snRNP
C	0005829	cellular_component	cytosol
C	0015030	cellular_component	Cajal body
C	0016020	cellular_component	membrane
C	0016607	cellular_component	nuclear speck
C	0030623	molecular_function	U5 snRNA binding
C	0042220	biological_process	response to cocaine
C	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
C	0048025	biological_process	negative regulation of mRNA splicing, via spliceosome
C	0071005	cellular_component	U2-type precatalytic spliceosome
C	0071006	cellular_component	U2-type catalytic step 1 spliceosome
C	0071007	cellular_component	U2-type catalytic step 2 spliceosome
C	0071009	cellular_component	U4atac/U6atac x U5 tri-snRNP complex
C	0071011	cellular_component	precatalytic spliceosome
C	0071013	cellular_component	catalytic step 2 spliceosome
C	0071014	cellular_component	post-mRNA release spliceosomal complex
C	0071016	cellular_component	U12-type precatalytic spliceosome
C	0071018	cellular_component	U12-type catalytic step 2 spliceosome
C	0071020	cellular_component	post-spliceosomal complex
C	0071466	biological_process	cellular response to xenobiotic stimulus
I	0000398	biological_process	mRNA splicing, via spliceosome
I	0003723	molecular_function	RNA binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0071005	cellular_component	U2-type precatalytic spliceosome
I	0071011	cellular_component	precatalytic spliceosome
K	0000398	biological_process	mRNA splicing, via spliceosome
K	0001527	cellular_component	microfibril
K	0003723	molecular_function	RNA binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005684	cellular_component	U2-type spliceosomal complex
K	0071005	cellular_component	U2-type precatalytic spliceosome
K	0140149	cellular_component	non-collagenous component of interstitial matrix
P	0000398	biological_process	mRNA splicing, via spliceosome
P	0000974	cellular_component	Prp19 complex
P	0003723	molecular_function	RNA binding
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005681	cellular_component	spliceosomal complex
P	0005737	cellular_component	cytoplasm
P	0017070	molecular_function	U6 snRNA binding
P	0033120	biological_process	positive regulation of RNA splicing
P	0036002	molecular_function	pre-mRNA binding
P	0042307	biological_process	positive regulation of protein import into nucleus
P	0045292	biological_process	mRNA cis splicing, via spliceosome
P	0046827	biological_process	positive regulation of protein export from nucleus
P	0048306	molecular_function	calcium-dependent protein binding
P	0071006	cellular_component	U2-type catalytic step 1 spliceosome
P	0071007	cellular_component	U2-type catalytic step 2 spliceosome
P	0071013	cellular_component	catalytic step 2 spliceosome
P	0071014	cellular_component	post-mRNA release spliceosomal complex
P	0071020	cellular_component	post-spliceosomal complex
P	0071466	biological_process	cellular response to xenobiotic stimulus
P7	0000398	biological_process	mRNA splicing, via spliceosome
P7	0003723	molecular_function	RNA binding
P7	0005515	molecular_function	protein binding
P7	0005634	cellular_component	nucleus
P7	0005654	cellular_component	nucleoplasm
P7	0005681	cellular_component	spliceosomal complex
P7	0016607	cellular_component	nuclear speck
P7	0071006	cellular_component	U2-type catalytic step 1 spliceosome
P7	0071007	cellular_component	U2-type catalytic step 2 spliceosome
P7	0071013	cellular_component	catalytic step 2 spliceosome
P7	0071014	cellular_component	post-mRNA release spliceosomal complex
P7	0071020	cellular_component	post-spliceosomal complex
P7	1990403	biological_process	embryonic brain development
q	0000398	biological_process	mRNA splicing, via spliceosome
q	0005515	molecular_function	protein binding
q	0005634	cellular_component	nucleus
q	0005654	cellular_component	nucleoplasm
q	0005737	cellular_component	cytoplasm
q	0005829	cellular_component	cytosol
q	0015030	cellular_component	Cajal body
q	0031386	molecular_function	protein tag activity
q	1903749	biological_process	positive regulation of protein localization to mitochondrion
Q	0000398	biological_process	mRNA splicing, via spliceosome
Q	0000785	cellular_component	chromatin
Q	0003713	molecular_function	transcription coactivator activity
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005681	cellular_component	spliceosomal complex
Q	0016922	molecular_function	nuclear receptor binding
Q	0045893	biological_process	positive regulation of DNA-templated transcription
Q	0071006	cellular_component	U2-type catalytic step 1 spliceosome
Q	0071007	cellular_component	U2-type catalytic step 2 spliceosome
Q	0071014	cellular_component	post-mRNA release spliceosomal complex
Q	0071020	cellular_component	post-spliceosomal complex
r	0000398	biological_process	mRNA splicing, via spliceosome
r	0005515	molecular_function	protein binding
r	0005634	cellular_component	nucleus
r	0005654	cellular_component	nucleoplasm
r	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
r	0071005	cellular_component	U2-type precatalytic spliceosome
S1	0000122	biological_process	negative regulation of transcription by RNA polymerase II
S1	0000398	biological_process	mRNA splicing, via spliceosome
S1	0003713	molecular_function	transcription coactivator activity
S1	0003714	molecular_function	transcription corepressor activity
S1	0003723	molecular_function	RNA binding
S1	0005112	molecular_function	Notch binding
S1	0005515	molecular_function	protein binding
S1	0005634	cellular_component	nucleus
S1	0005654	cellular_component	nucleoplasm
S1	0005681	cellular_component	spliceosomal complex
S1	0006357	biological_process	regulation of transcription by RNA polymerase II
S1	0008024	cellular_component	cyclin/CDK positive transcription elongation factor complex
S1	0016363	cellular_component	nuclear matrix
S1	0016607	cellular_component	nuclear speck
S1	0016922	molecular_function	nuclear receptor binding
S1	0019899	molecular_function	enzyme binding
S1	0030511	biological_process	positive regulation of transforming growth factor beta receptor signaling pathway
S1	0042771	biological_process	intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
S1	0042809	molecular_function	nuclear vitamin D receptor binding
S1	0042974	molecular_function	nuclear retinoic acid receptor binding
S1	0043923	biological_process	host-mediated activation of viral transcription
S1	0045892	biological_process	negative regulation of DNA-templated transcription
S1	0045944	biological_process	positive regulation of transcription by RNA polymerase II
S1	0046332	molecular_function	SMAD binding
S1	0048026	biological_process	positive regulation of mRNA splicing, via spliceosome
S1	0048255	biological_process	mRNA stabilization
S1	0048384	biological_process	retinoic acid receptor signaling pathway
S1	0048385	biological_process	regulation of retinoic acid receptor signaling pathway
S1	0050681	molecular_function	nuclear androgen receptor binding
S1	0050769	biological_process	positive regulation of neurogenesis
S1	0070562	biological_process	regulation of vitamin D receptor signaling pathway
S1	0070564	biological_process	positive regulation of vitamin D receptor signaling pathway
S1	0071006	cellular_component	U2-type catalytic step 1 spliceosome
S1	0071007	cellular_component	U2-type catalytic step 2 spliceosome
S1	0071013	cellular_component	catalytic step 2 spliceosome
S1	0071014	cellular_component	post-mRNA release spliceosomal complex
S1	0071018	cellular_component	U12-type catalytic step 2 spliceosome
S1	0071020	cellular_component	post-spliceosomal complex
S1	0071300	biological_process	cellular response to retinoic acid
t	0000209	biological_process	protein polyubiquitination
t	0000398	biological_process	mRNA splicing, via spliceosome
t	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
t	0005515	molecular_function	protein binding
t	0005634	cellular_component	nucleus
t	0005654	cellular_component	nucleoplasm
t	0005681	cellular_component	spliceosomal complex
t	0005737	cellular_component	cytoplasm
t	0005796	cellular_component	Golgi lumen
t	0005886	cellular_component	plasma membrane
t	0016567	biological_process	protein ubiquitination
t	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
t	0061630	molecular_function	ubiquitin protein ligase activity
t	0071006	cellular_component	U2-type catalytic step 1 spliceosome
t	0071013	cellular_component	catalytic step 2 spliceosome
t	0071018	cellular_component	U12-type catalytic step 2 spliceosome
t	0072659	biological_process	protein localization to plasma membrane
T	0000122	biological_process	negative regulation of transcription by RNA polymerase II
T	0003711	molecular_function	transcription elongation factor activity
T	0003712	molecular_function	transcription coregulator activity
T	0003713	molecular_function	transcription coactivator activity
T	0003714	molecular_function	transcription corepressor activity
T	0003723	molecular_function	RNA binding
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0006397	biological_process	mRNA processing
T	0008380	biological_process	RNA splicing
T	0016607	cellular_component	nuclear speck
T	0032968	biological_process	positive regulation of transcription elongation by RNA polymerase II
T	0034244	biological_process	negative regulation of transcription elongation by RNA polymerase II
T	0042802	molecular_function	identical protein binding
T	0044390	molecular_function	ubiquitin-like protein conjugating enzyme binding
T	0045944	biological_process	positive regulation of transcription by RNA polymerase II
T	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
T	0070063	molecular_function	RNA polymerase binding
X	0003697	molecular_function	single-stranded DNA binding
X	0003723	molecular_function	RNA binding
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0008380	biological_process	RNA splicing
X	0019888	molecular_function	protein phosphatase regulator activity
X	0050699	molecular_function	WW domain binding

Functional Information from PROSITE/UniProt

site_id	PS00028
Number of Residues	23
Details	ZINC_FINGER_C2H2_1 Zinc finger C2H2 type domain signature. Cnv..CdcvVkdsinfldHingkkH

Chain	Residue	Details
r	CYS82-HIS104

---

site_id	PS00170
Number of Residues	18
Details	CSA_PPIASE_1 Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature. YdgTiFHRSIrnFViQGG

Chain	Residue	Details
t	TYR316-GLY333

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LLSCsmDcKIKLWEV

Chain	Residue	Details
P7	LEU304-VAL318

---

site_id	PS00997
Number of Residues	23
Details	G10_1 G10 protein signature 1. LCClRCiqtrDtNfgtnCiCRVP

Chain	Residue	Details
Q	LEU100-PRO122

---

site_id	PS00998
Number of Residues	10
Details	G10_2 G10 protein signature 2. CtHCGCRGCS

Chain	Residue	Details
Q	CYS134-SER143

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	183
Details	Domain: {"description":"Helicase ATP-binding 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	237
Details	Domain: {"description":"Helicase C-terminal 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00542","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	305
Details	Domain: {"description":"SEC63 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	Motif: {"description":"DEIH box"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23045696","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4F93","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"F1LNJ2","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	282
Details	Domain: {"description":"tr-type G","evidences":[{"source":"PROSITE-ProRule","id":"PRU01059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	14
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	178
Details	Region: {"description":"N-terminal protein interaction domain","evidences":[{"source":"PubMed","id":"26673105","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	12
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	8
Details	Region: {"description":"Interaction with AR","evidences":[{"source":"PubMed","id":"28076346","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	56
Details	Domain: {"description":"FF 4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	56
Details	Domain: {"description":"FF 5"}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	65
Details	Domain: {"description":"FF 6"}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	25
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	9
Details	Compositional bias: {"description":"Basic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	72
Details	Domain: {"description":"Ubiquitin-like"}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	24
Details	Zinc finger: {"description":"Matrin-type"}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25772364","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	131
Details	Domain: {"description":"MPN","evidences":[{"source":"PROSITE-ProRule","id":"PRU01182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	491
Details	Region: {"description":"Reverse transcriptase homology domain"}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	171
Details	Region: {"description":"Restriction endonuclease homology domain"}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	34
Details	Region: {"description":"Required for interaction with EFTUD2 and SNRNP200","evidences":[{"source":"PubMed","id":"17317632","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI27
Number of Residues	1
Details	Modified residue: {"description":"N6,N6-dimethyllysine","evidences":[{"source":"PubMed","id":"24129315","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI28
Number of Residues	27
Details	Zinc finger: {"description":"C3H1-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00723","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI29
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25218447","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI30
Number of Residues	1
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25218447","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25772364","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---