9QBW
Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with Carfilzomib
This is a non-PDB format compatible entry.
Functional Information from PROSITE/UniProt
| site_id | PS00388 |
| Number of Residues | 23 |
| Details | PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YsfslTtFSPsGKlgQIDYAltA |
| Chain | Residue | Details |
| A | TYR5-ALA27 | |
| G | TYR3-ALA25 | |
| D | TYR0-ALA22 | |
| C | TYR2-ALA24 | |
| F | TYR4-ALA26 | |
| E | TYR5-ALA27 | |
| B | TYR5-SER27 |
| site_id | PS00854 |
| Number of Residues | 48 |
| Details | PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LGIrVqdSVILASSkavtrgisvlkdsdd.KtrqlsphtlmsfaGeaGD |
| Chain | Residue | Details |
| J | LEU5-ASP52 | |
| L | LEU13-ASP60 | |
| C | VAL32-ASP79 | |
| I | VAL12-ASP58 | |
| b | MET4-ASP51 | |
| H | VAL4-ASP51 | |
| B | ILE35-ASP82 | |
| K | LEU4-ASP51 | |
| a | ILE12-ASP59 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 24 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22106047","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17287358","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Binding site: {} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 177 |
| Chain | Residue | Details |
| H | ASP17 | activator, steric locator |
| H | ARG19 | activator |
| H | LYS33 | activator, electrostatic stabiliser |
| H | GLY47 | electrostatic stabiliser |
| H | SER129 | activator, electrostatic stabiliser |
| H | ASP166 | activator, steric locator |
| H | SER169 | activator, electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 7 |
| Details | M-CSA 177 |
| Chain | Residue | Details |
| V | ASP17 | activator, steric locator |
| V | ARG19 | activator |
| V | LYS33 | activator, electrostatic stabiliser |
| V | GLY47 | electrostatic stabiliser |
| V | SER129 | activator, electrostatic stabiliser |
| V | ASP166 | activator, steric locator |
| V | SER169 | activator, electrostatic stabiliser |
