9Q9J

Cryo-EM structure of human Mre11-Rad50-Nbs1 (MRN) complex bound to DNA and telomeric factor TRF2 fragment (438-542)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000014	molecular_function	single-stranded DNA endodeoxyribonuclease activity
A	0000019	biological_process	regulation of mitotic recombination
A	0000166	molecular_function	nucleotide binding
A	0000722	biological_process	telomere maintenance via recombination
A	0000723	biological_process	telomere maintenance
A	0000724	biological_process	double-strand break repair via homologous recombination
A	0000729	biological_process	DNA double-strand break processing
A	0000781	cellular_component	chromosome, telomeric region
A	0000794	cellular_component	condensed nuclear chromosome
A	0003677	molecular_function	DNA binding
A	0003678	molecular_function	DNA helicase activity
A	0003691	molecular_function	double-stranded telomeric DNA binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0006281	biological_process	DNA repair
A	0006302	biological_process	double-strand break repair
A	0006310	biological_process	DNA recombination
A	0006974	biological_process	DNA damage response
A	0007004	biological_process	telomere maintenance via telomerase
A	0007131	biological_process	reciprocal meiotic recombination
A	0008408	molecular_function	3'-5' exonuclease activity
A	0016020	cellular_component	membrane
A	0016604	cellular_component	nuclear body
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0030870	cellular_component	Mre11 complex
A	0031860	biological_process	telomeric 3' overhang formation
A	0032206	biological_process	positive regulation of telomere maintenance
A	0035825	biological_process	homologous recombination
A	0035861	cellular_component	site of double-strand break
A	0042802	molecular_function	identical protein binding
A	0043047	molecular_function	single-stranded telomeric DNA binding
A	0043539	molecular_function	protein serine/threonine kinase activator activity
A	0044818	biological_process	mitotic G2/M transition checkpoint
A	0046872	molecular_function	metal ion binding
A	0051276	biological_process	chromosome organization
A	0051321	biological_process	meiotic cell cycle
A	0051880	molecular_function	G-quadruplex DNA binding
A	0062176	biological_process	R-loop processing
A	0070192	biological_process	chromosome organization involved in meiotic cell cycle
A	0098687	cellular_component	chromosomal region
A	0110025	biological_process	DNA strand resection involved in replication fork processing
A	1904354	biological_process	negative regulation of telomere capping
A	2000781	biological_process	positive regulation of double-strand break repair
B	0000014	molecular_function	single-stranded DNA endodeoxyribonuclease activity
B	0000019	biological_process	regulation of mitotic recombination
B	0000166	molecular_function	nucleotide binding
B	0000722	biological_process	telomere maintenance via recombination
B	0000723	biological_process	telomere maintenance
B	0000724	biological_process	double-strand break repair via homologous recombination
B	0000729	biological_process	DNA double-strand break processing
B	0000781	cellular_component	chromosome, telomeric region
B	0000794	cellular_component	condensed nuclear chromosome
B	0003677	molecular_function	DNA binding
B	0003678	molecular_function	DNA helicase activity
B	0003691	molecular_function	double-stranded telomeric DNA binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005694	cellular_component	chromosome
B	0006281	biological_process	DNA repair
B	0006302	biological_process	double-strand break repair
B	0006310	biological_process	DNA recombination
B	0006974	biological_process	DNA damage response
B	0007004	biological_process	telomere maintenance via telomerase
B	0007131	biological_process	reciprocal meiotic recombination
B	0008408	molecular_function	3'-5' exonuclease activity
B	0016020	cellular_component	membrane
B	0016604	cellular_component	nuclear body
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0030870	cellular_component	Mre11 complex
B	0031860	biological_process	telomeric 3' overhang formation
B	0032206	biological_process	positive regulation of telomere maintenance
B	0035825	biological_process	homologous recombination
B	0035861	cellular_component	site of double-strand break
B	0042802	molecular_function	identical protein binding
B	0043047	molecular_function	single-stranded telomeric DNA binding
B	0043539	molecular_function	protein serine/threonine kinase activator activity
B	0044818	biological_process	mitotic G2/M transition checkpoint
B	0046872	molecular_function	metal ion binding
B	0051276	biological_process	chromosome organization
B	0051321	biological_process	meiotic cell cycle
B	0051880	molecular_function	G-quadruplex DNA binding
B	0062176	biological_process	R-loop processing
B	0070192	biological_process	chromosome organization involved in meiotic cell cycle
B	0098687	cellular_component	chromosomal region
B	0110025	biological_process	DNA strand resection involved in replication fork processing
B	1904354	biological_process	negative regulation of telomere capping
B	2000781	biological_process	positive regulation of double-strand break repair
D	0000014	molecular_function	single-stranded DNA endodeoxyribonuclease activity
D	0000019	biological_process	regulation of mitotic recombination
D	0000723	biological_process	telomere maintenance
D	0000724	biological_process	double-strand break repair via homologous recombination
D	0000729	biological_process	DNA double-strand break processing
D	0000781	cellular_component	chromosome, telomeric region
D	0003677	molecular_function	DNA binding
D	0003678	molecular_function	DNA helicase activity
D	0003690	molecular_function	double-stranded DNA binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004520	molecular_function	DNA endonuclease activity
D	0004527	molecular_function	exonuclease activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005657	cellular_component	replication fork
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006281	biological_process	DNA repair
D	0006302	biological_process	double-strand break repair
D	0006303	biological_process	double-strand break repair via nonhomologous end joining
D	0006310	biological_process	DNA recombination
D	0006974	biological_process	DNA damage response
D	0007004	biological_process	telomere maintenance via telomerase
D	0007062	biological_process	sister chromatid cohesion
D	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
D	0007131	biological_process	reciprocal meiotic recombination
D	0008296	molecular_function	3'-5'-DNA exonuclease activity
D	0008408	molecular_function	3'-5' exonuclease activity
D	0016605	cellular_component	PML body
D	0016787	molecular_function	hydrolase activity
D	0030870	cellular_component	Mre11 complex
D	0031573	biological_process	mitotic intra-S DNA damage checkpoint signaling
D	0031860	biological_process	telomeric 3' overhang formation
D	0032206	biological_process	positive regulation of telomere maintenance
D	0035825	biological_process	homologous recombination
D	0035861	cellular_component	site of double-strand break
D	0042138	biological_process	meiotic DNA double-strand break formation
D	0042802	molecular_function	identical protein binding
D	0043066	biological_process	negative regulation of apoptotic process
D	0044818	biological_process	mitotic G2/M transition checkpoint
D	0045296	molecular_function	cadherin binding
D	0051276	biological_process	chromosome organization
D	0051321	biological_process	meiotic cell cycle
D	0062176	biological_process	R-loop processing
D	0097552	biological_process	mitochondrial double-strand break repair via homologous recombination
D	0098687	cellular_component	chromosomal region
D	0110025	biological_process	DNA strand resection involved in replication fork processing
D	2000781	biological_process	positive regulation of double-strand break repair
D	2001033	biological_process	negative regulation of double-strand break repair via nonhomologous end joining
E	0000014	molecular_function	single-stranded DNA endodeoxyribonuclease activity
E	0000019	biological_process	regulation of mitotic recombination
E	0000723	biological_process	telomere maintenance
E	0000724	biological_process	double-strand break repair via homologous recombination
E	0000729	biological_process	DNA double-strand break processing
E	0000781	cellular_component	chromosome, telomeric region
E	0003677	molecular_function	DNA binding
E	0003678	molecular_function	DNA helicase activity
E	0003690	molecular_function	double-stranded DNA binding
E	0004518	molecular_function	nuclease activity
E	0004519	molecular_function	endonuclease activity
E	0004520	molecular_function	DNA endonuclease activity
E	0004527	molecular_function	exonuclease activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005657	cellular_component	replication fork
E	0005694	cellular_component	chromosome
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006302	biological_process	double-strand break repair
E	0006303	biological_process	double-strand break repair via nonhomologous end joining
E	0006310	biological_process	DNA recombination
E	0006974	biological_process	DNA damage response
E	0007004	biological_process	telomere maintenance via telomerase
E	0007062	biological_process	sister chromatid cohesion
E	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
E	0007131	biological_process	reciprocal meiotic recombination
E	0008296	molecular_function	3'-5'-DNA exonuclease activity
E	0008408	molecular_function	3'-5' exonuclease activity
E	0016605	cellular_component	PML body
E	0016787	molecular_function	hydrolase activity
E	0030870	cellular_component	Mre11 complex
E	0031573	biological_process	mitotic intra-S DNA damage checkpoint signaling
E	0031860	biological_process	telomeric 3' overhang formation
E	0032206	biological_process	positive regulation of telomere maintenance
E	0035825	biological_process	homologous recombination
E	0035861	cellular_component	site of double-strand break
E	0042138	biological_process	meiotic DNA double-strand break formation
E	0042802	molecular_function	identical protein binding
E	0043066	biological_process	negative regulation of apoptotic process
E	0044818	biological_process	mitotic G2/M transition checkpoint
E	0045296	molecular_function	cadherin binding
E	0051276	biological_process	chromosome organization
E	0051321	biological_process	meiotic cell cycle
E	0062176	biological_process	R-loop processing
E	0097552	biological_process	mitochondrial double-strand break repair via homologous recombination
E	0098687	cellular_component	chromosomal region
E	0110025	biological_process	DNA strand resection involved in replication fork processing
E	2000781	biological_process	positive regulation of double-strand break repair
E	2001033	biological_process	negative regulation of double-strand break repair via nonhomologous end joining
F	0000077	biological_process	DNA damage checkpoint signaling
F	0000723	biological_process	telomere maintenance
F	0000724	biological_process	double-strand break repair via homologous recombination
F	0000729	biological_process	DNA double-strand break processing
F	0000781	cellular_component	chromosome, telomeric region
F	0001701	biological_process	in utero embryonic development
F	0001832	biological_process	blastocyst growth
F	0003684	molecular_function	damaged DNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005657	cellular_component	replication fork
F	0005694	cellular_component	chromosome
F	0005730	cellular_component	nucleolus
F	0005829	cellular_component	cytosol
F	0006281	biological_process	DNA repair
F	0006302	biological_process	double-strand break repair
F	0006974	biological_process	DNA damage response
F	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
F	0016605	cellular_component	PML body
F	0030174	biological_process	regulation of DNA-templated DNA replication initiation
F	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
F	0030870	cellular_component	Mre11 complex
F	0031848	biological_process	protection from non-homologous end joining at telomere
F	0031860	biological_process	telomeric 3' overhang formation
F	0032206	biological_process	positive regulation of telomere maintenance
F	0035825	biological_process	homologous recombination
F	0035861	cellular_component	site of double-strand break
F	0042393	molecular_function	histone binding
F	0042405	cellular_component	nuclear inclusion body
F	0043247	biological_process	telomere maintenance in response to DNA damage
F	0043539	molecular_function	protein serine/threonine kinase activator activity
F	0044818	biological_process	mitotic G2/M transition checkpoint
F	0045190	biological_process	isotype switching
F	0051321	biological_process	meiotic cell cycle
F	0051726	biological_process	regulation of cell cycle
F	0062176	biological_process	R-loop processing
F	0070534	biological_process	protein K63-linked ubiquitination
F	0090656	biological_process	t-circle formation
F	0090737	biological_process	telomere maintenance via telomere trimming
F	0097681	biological_process	double-strand break repair via alternative nonhomologous end joining
F	0098687	cellular_component	chromosomal region
F	0110025	biological_process	DNA strand resection involved in replication fork processing
F	0140031	molecular_function	phosphorylation-dependent protein binding
F	0140297	molecular_function	DNA-binding transcription factor binding
F	0140463	molecular_function	chromatin-protein adaptor activity
F	1904354	biological_process	negative regulation of telomere capping
F	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
F	1990166	biological_process	protein localization to site of double-strand break
F	2000781	biological_process	positive regulation of double-strand break repair

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	9
Details	Motif: {"description":"FxF/Y motif","evidences":[{"source":"PubMed","id":"15758953","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"35076389","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"30952868","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22464731","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"G0SHW7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	60
Details	Region: {"description":"Interaction with NBN","evidences":[{"source":"PubMed","id":"22078559","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Active site: {"description":"Proton donor","evidences":[{"source":"UniProtKB","id":"Q61216","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22078559","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36577401","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3T1I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8BAH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"36050397","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"36563124","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)","evidences":[{"source":"PubMed","id":"36050397","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---