9Q3E

Cryo-EM structure of RotavirusA NSP1-ELOB-ELOC-CUL3

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000151	cellular_component	ubiquitin ligase complex
B	0001222	molecular_function	transcription corepressor binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006367	biological_process	transcription initiation at RNA polymerase II promoter
B	0006368	biological_process	transcription elongation by RNA polymerase II
B	0016567	biological_process	protein ubiquitination
B	0030891	cellular_component	VCB complex
B	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
B	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
B	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0065003	biological_process	protein-containing complex assembly
B	0070449	cellular_component	elongin complex
B	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
B	0140958	biological_process	target-directed miRNA degradation
B	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
B	2000104	biological_process	negative regulation of DNA-templated DNA replication
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000151	cellular_component	ubiquitin ligase complex
C	0001222	molecular_function	transcription corepressor binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0006367	biological_process	transcription initiation at RNA polymerase II promoter
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0016567	biological_process	protein ubiquitination
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
C	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0070449	cellular_component	elongin complex
C	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
C	0140958	biological_process	target-directed miRNA degradation
C	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
C	2000104	biological_process	negative regulation of DNA-templated DNA replication
L	0000209	biological_process	protein polyubiquitination
L	0000278	biological_process	mitotic cell cycle
L	0000922	cellular_component	spindle pole
L	0004842	molecular_function	ubiquitin-protein transferase activity
L	0005112	molecular_function	Notch binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005737	cellular_component	cytoplasm
L	0005794	cellular_component	Golgi apparatus
L	0005813	cellular_component	centrosome
L	0005819	cellular_component	spindle
L	0005827	cellular_component	polar microtubule
L	0005829	cellular_component	cytosol
L	0005886	cellular_component	plasma membrane
L	0006511	biological_process	ubiquitin-dependent protein catabolic process
L	0006513	biological_process	protein monoubiquitination
L	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
L	0006915	biological_process	apoptotic process
L	0006950	biological_process	response to stress
L	0007080	biological_process	mitotic metaphase chromosome alignment
L	0007165	biological_process	signal transduction
L	0007229	biological_process	integrin-mediated signaling pathway
L	0008284	biological_process	positive regulation of cell population proliferation
L	0010468	biological_process	regulation of gene expression
L	0015630	cellular_component	microtubule cytoskeleton
L	0016020	cellular_component	membrane
L	0016477	biological_process	cell migration
L	0016567	biological_process	protein ubiquitination
L	0017145	biological_process	stem cell division
L	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
L	0031208	molecular_function	POZ domain binding
L	0031398	biological_process	positive regulation of protein ubiquitination
L	0031461	cellular_component	cullin-RING ubiquitin ligase complex
L	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
L	0031514	cellular_component	motile cilium
L	0031625	molecular_function	ubiquitin protein ligase binding
L	0031648	biological_process	protein destabilization
L	0032467	biological_process	positive regulation of cytokinesis
L	0032480	biological_process	negative regulation of type I interferon production
L	0034599	biological_process	cellular response to oxidative stress
L	0035024	biological_process	negative regulation of Rho protein signal transduction
L	0036126	cellular_component	sperm flagellum
L	0040016	biological_process	embryonic cleavage
L	0043149	biological_process	stress fiber assembly
L	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
L	0045724	biological_process	positive regulation of cilium assembly
L	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
L	0048208	biological_process	COPII vesicle coat assembly
L	0051865	biological_process	protein autoubiquitination
L	0061028	biological_process	establishment of endothelial barrier
L	0061630	molecular_function	ubiquitin protein ligase activity
L	0070062	cellular_component	extracellular exosome
L	0070936	biological_process	protein K48-linked ubiquitination
L	0071230	biological_process	cellular response to amino acid stimulus
L	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
L	0072686	cellular_component	mitotic spindle
L	0097193	biological_process	intrinsic apoptotic signaling pathway
L	0160072	molecular_function	ubiquitin ligase complex scaffold activity
L	1900076	biological_process	regulation of cellular response to insulin stimulus
L	1901992	biological_process	positive regulation of mitotic cell cycle phase transition
L	1902523	biological_process	positive regulation of protein K63-linked ubiquitination
L	1904263	biological_process	positive regulation of TORC1 signaling
N	0003723	molecular_function	RNA binding
N	0030430	cellular_component	host cell cytoplasm
N	0039548	biological_process	symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
N	0039557	biological_process	symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity
N	0044163	cellular_component	host cytoskeleton
N	0046872	molecular_function	metal ion binding
N	0052031	biological_process	symbiont-mediated perturbation of host defense response
N	0052170	biological_process	symbiont-mediated suppression of host innate immune response
N	0085034	biological_process	symbiont-mediated suppression of host NF-kappaB cascade

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkrIegLIEReYLaRtpedrkvYtYvA

Chain	Residue	Details
L	ILE741-ALA768

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	80
Details	Region: {"description":"RNA-binding","evidences":[{"source":"HAMAP-Rule","id":"MF_04088","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	37
Details	Region: {"description":"Zinc-binding domain","evidences":[{"source":"HAMAP-Rule","id":"MF_04088","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	65
Details	Domain: {"description":"Ubiquitin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P62869","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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