9PDD

22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 29

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000149	molecular_function	SNARE binding
A	0000166	molecular_function	nucleotide binding
A	0001921	biological_process	positive regulation of receptor recycling
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005794	cellular_component	Golgi apparatus
A	0005795	cellular_component	Golgi stack
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006813	biological_process	potassium ion transport
A	0006886	biological_process	intracellular protein transport
A	0006891	biological_process	intra-Golgi vesicle-mediated transport
A	0015031	biological_process	protein transport
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0017075	molecular_function	syntaxin-1 binding
A	0019901	molecular_function	protein kinase binding
A	0019905	molecular_function	syntaxin binding
A	0030165	molecular_function	PDZ domain binding
A	0030496	cellular_component	midbody
A	0035255	molecular_function	ionotropic glutamate receptor binding
A	0035494	biological_process	SNARE complex disassembly
A	0042802	molecular_function	identical protein binding
A	0043001	biological_process	Golgi to plasma membrane protein transport
A	0044877	molecular_function	protein-containing complex binding
A	0045732	biological_process	positive regulation of protein catabolic process
A	0046872	molecular_function	metal ion binding
A	0140545	molecular_function	ATP-dependent protein disaggregase activity
B	0000149	molecular_function	SNARE binding
B	0000166	molecular_function	nucleotide binding
B	0001921	biological_process	positive regulation of receptor recycling
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005794	cellular_component	Golgi apparatus
B	0005795	cellular_component	Golgi stack
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006813	biological_process	potassium ion transport
B	0006886	biological_process	intracellular protein transport
B	0006891	biological_process	intra-Golgi vesicle-mediated transport
B	0015031	biological_process	protein transport
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0017075	molecular_function	syntaxin-1 binding
B	0019901	molecular_function	protein kinase binding
B	0019905	molecular_function	syntaxin binding
B	0030165	molecular_function	PDZ domain binding
B	0030496	cellular_component	midbody
B	0035255	molecular_function	ionotropic glutamate receptor binding
B	0035494	biological_process	SNARE complex disassembly
B	0042802	molecular_function	identical protein binding
B	0043001	biological_process	Golgi to plasma membrane protein transport
B	0044877	molecular_function	protein-containing complex binding
B	0045732	biological_process	positive regulation of protein catabolic process
B	0046872	molecular_function	metal ion binding
B	0140545	molecular_function	ATP-dependent protein disaggregase activity
C	0000149	molecular_function	SNARE binding
C	0000166	molecular_function	nucleotide binding
C	0001921	biological_process	positive regulation of receptor recycling
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005795	cellular_component	Golgi stack
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006813	biological_process	potassium ion transport
C	0006886	biological_process	intracellular protein transport
C	0006891	biological_process	intra-Golgi vesicle-mediated transport
C	0015031	biological_process	protein transport
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0017075	molecular_function	syntaxin-1 binding
C	0019901	molecular_function	protein kinase binding
C	0019905	molecular_function	syntaxin binding
C	0030165	molecular_function	PDZ domain binding
C	0030496	cellular_component	midbody
C	0035255	molecular_function	ionotropic glutamate receptor binding
C	0035494	biological_process	SNARE complex disassembly
C	0042802	molecular_function	identical protein binding
C	0043001	biological_process	Golgi to plasma membrane protein transport
C	0044877	molecular_function	protein-containing complex binding
C	0045732	biological_process	positive regulation of protein catabolic process
C	0046872	molecular_function	metal ion binding
C	0140545	molecular_function	ATP-dependent protein disaggregase activity
D	0000149	molecular_function	SNARE binding
D	0000166	molecular_function	nucleotide binding
D	0001921	biological_process	positive regulation of receptor recycling
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005794	cellular_component	Golgi apparatus
D	0005795	cellular_component	Golgi stack
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006813	biological_process	potassium ion transport
D	0006886	biological_process	intracellular protein transport
D	0006891	biological_process	intra-Golgi vesicle-mediated transport
D	0015031	biological_process	protein transport
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0017075	molecular_function	syntaxin-1 binding
D	0019901	molecular_function	protein kinase binding
D	0019905	molecular_function	syntaxin binding
D	0030165	molecular_function	PDZ domain binding
D	0030496	cellular_component	midbody
D	0035255	molecular_function	ionotropic glutamate receptor binding
D	0035494	biological_process	SNARE complex disassembly
D	0042802	molecular_function	identical protein binding
D	0043001	biological_process	Golgi to plasma membrane protein transport
D	0044877	molecular_function	protein-containing complex binding
D	0045732	biological_process	positive regulation of protein catabolic process
D	0046872	molecular_function	metal ion binding
D	0140545	molecular_function	ATP-dependent protein disaggregase activity
E	0000149	molecular_function	SNARE binding
E	0000166	molecular_function	nucleotide binding
E	0001921	biological_process	positive regulation of receptor recycling
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005794	cellular_component	Golgi apparatus
E	0005795	cellular_component	Golgi stack
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006813	biological_process	potassium ion transport
E	0006886	biological_process	intracellular protein transport
E	0006891	biological_process	intra-Golgi vesicle-mediated transport
E	0015031	biological_process	protein transport
E	0016787	molecular_function	hydrolase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0017075	molecular_function	syntaxin-1 binding
E	0019901	molecular_function	protein kinase binding
E	0019905	molecular_function	syntaxin binding
E	0030165	molecular_function	PDZ domain binding
E	0030496	cellular_component	midbody
E	0035255	molecular_function	ionotropic glutamate receptor binding
E	0035494	biological_process	SNARE complex disassembly
E	0042802	molecular_function	identical protein binding
E	0043001	biological_process	Golgi to plasma membrane protein transport
E	0044877	molecular_function	protein-containing complex binding
E	0045732	biological_process	positive regulation of protein catabolic process
E	0046872	molecular_function	metal ion binding
E	0140545	molecular_function	ATP-dependent protein disaggregase activity
F	0000149	molecular_function	SNARE binding
F	0000166	molecular_function	nucleotide binding
F	0001921	biological_process	positive regulation of receptor recycling
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005794	cellular_component	Golgi apparatus
F	0005795	cellular_component	Golgi stack
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006813	biological_process	potassium ion transport
F	0006886	biological_process	intracellular protein transport
F	0006891	biological_process	intra-Golgi vesicle-mediated transport
F	0015031	biological_process	protein transport
F	0016787	molecular_function	hydrolase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0017075	molecular_function	syntaxin-1 binding
F	0019901	molecular_function	protein kinase binding
F	0019905	molecular_function	syntaxin binding
F	0030165	molecular_function	PDZ domain binding
F	0030496	cellular_component	midbody
F	0035255	molecular_function	ionotropic glutamate receptor binding
F	0035494	biological_process	SNARE complex disassembly
F	0042802	molecular_function	identical protein binding
F	0043001	biological_process	Golgi to plasma membrane protein transport
F	0044877	molecular_function	protein-containing complex binding
F	0045732	biological_process	positive regulation of protein catabolic process
F	0046872	molecular_function	metal ion binding
F	0140545	molecular_function	ATP-dependent protein disaggregase activity
I	0000149	molecular_function	SNARE binding
I	0005483	molecular_function	soluble NSF attachment protein activity
I	0005515	molecular_function	protein binding
I	0005739	cellular_component	mitochondrion
I	0005886	cellular_component	plasma membrane
I	0006886	biological_process	intracellular protein transport
I	0007420	biological_process	brain development
I	0008021	cellular_component	synaptic vesicle
I	0010807	biological_process	regulation of synaptic vesicle priming
I	0015031	biological_process	protein transport
I	0016082	biological_process	synaptic vesicle priming
I	0016192	biological_process	vesicle-mediated transport
I	0019905	molecular_function	syntaxin binding
I	0030182	biological_process	neuron differentiation
I	0030425	cellular_component	dendrite
I	0031410	cellular_component	cytoplasmic vesicle
I	0031594	cellular_component	neuromuscular junction
I	0032781	biological_process	positive regulation of ATP-dependent activity
I	0032984	biological_process	protein-containing complex disassembly
I	0035249	biological_process	synaptic transmission, glutamatergic
I	0035494	biological_process	SNARE complex disassembly
I	0042599	cellular_component	lamellar body
I	0042734	cellular_component	presynaptic membrane
I	0043195	cellular_component	terminal bouton
I	0043204	cellular_component	perikaryon
I	0044877	molecular_function	protein-containing complex binding
I	0045176	biological_process	apical protein localization
I	0045211	cellular_component	postsynaptic membrane
I	0048787	cellular_component	presynaptic active zone membrane
I	0055093	biological_process	response to hyperoxia
I	0061025	biological_process	membrane fusion
I	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
I	0097060	cellular_component	synaptic membrane
I	0098793	cellular_component	presynapse
I	0098794	cellular_component	postsynapse
I	0098978	cellular_component	glutamatergic synapse
J	0000149	molecular_function	SNARE binding
J	0005483	molecular_function	soluble NSF attachment protein activity
J	0005515	molecular_function	protein binding
J	0005739	cellular_component	mitochondrion
J	0005886	cellular_component	plasma membrane
J	0006886	biological_process	intracellular protein transport
J	0007420	biological_process	brain development
J	0008021	cellular_component	synaptic vesicle
J	0010807	biological_process	regulation of synaptic vesicle priming
J	0015031	biological_process	protein transport
J	0016082	biological_process	synaptic vesicle priming
J	0016192	biological_process	vesicle-mediated transport
J	0019905	molecular_function	syntaxin binding
J	0030182	biological_process	neuron differentiation
J	0030425	cellular_component	dendrite
J	0031410	cellular_component	cytoplasmic vesicle
J	0031594	cellular_component	neuromuscular junction
J	0032781	biological_process	positive regulation of ATP-dependent activity
J	0032984	biological_process	protein-containing complex disassembly
J	0035249	biological_process	synaptic transmission, glutamatergic
J	0035494	biological_process	SNARE complex disassembly
J	0042599	cellular_component	lamellar body
J	0042734	cellular_component	presynaptic membrane
J	0043195	cellular_component	terminal bouton
J	0043204	cellular_component	perikaryon
J	0044877	molecular_function	protein-containing complex binding
J	0045176	biological_process	apical protein localization
J	0045211	cellular_component	postsynaptic membrane
J	0048787	cellular_component	presynaptic active zone membrane
J	0055093	biological_process	response to hyperoxia
J	0061025	biological_process	membrane fusion
J	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
J	0097060	cellular_component	synaptic membrane
J	0098793	cellular_component	presynapse
J	0098794	cellular_component	postsynapse
J	0098978	cellular_component	glutamatergic synapse
K	0000149	molecular_function	SNARE binding
K	0005483	molecular_function	soluble NSF attachment protein activity
K	0005515	molecular_function	protein binding
K	0005739	cellular_component	mitochondrion
K	0005886	cellular_component	plasma membrane
K	0006886	biological_process	intracellular protein transport
K	0007420	biological_process	brain development
K	0008021	cellular_component	synaptic vesicle
K	0010807	biological_process	regulation of synaptic vesicle priming
K	0015031	biological_process	protein transport
K	0016082	biological_process	synaptic vesicle priming
K	0016192	biological_process	vesicle-mediated transport
K	0019905	molecular_function	syntaxin binding
K	0030182	biological_process	neuron differentiation
K	0030425	cellular_component	dendrite
K	0031410	cellular_component	cytoplasmic vesicle
K	0031594	cellular_component	neuromuscular junction
K	0032781	biological_process	positive regulation of ATP-dependent activity
K	0032984	biological_process	protein-containing complex disassembly
K	0035249	biological_process	synaptic transmission, glutamatergic
K	0035494	biological_process	SNARE complex disassembly
K	0042599	cellular_component	lamellar body
K	0042734	cellular_component	presynaptic membrane
K	0043195	cellular_component	terminal bouton
K	0043204	cellular_component	perikaryon
K	0044877	molecular_function	protein-containing complex binding
K	0045176	biological_process	apical protein localization
K	0045211	cellular_component	postsynaptic membrane
K	0048787	cellular_component	presynaptic active zone membrane
K	0055093	biological_process	response to hyperoxia
K	0061025	biological_process	membrane fusion
K	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
K	0097060	cellular_component	synaptic membrane
K	0098793	cellular_component	presynapse
K	0098794	cellular_component	postsynapse
K	0098978	cellular_component	glutamatergic synapse
L	0000149	molecular_function	SNARE binding
L	0005483	molecular_function	soluble NSF attachment protein activity
L	0005515	molecular_function	protein binding
L	0005739	cellular_component	mitochondrion
L	0005886	cellular_component	plasma membrane
L	0006886	biological_process	intracellular protein transport
L	0007420	biological_process	brain development
L	0008021	cellular_component	synaptic vesicle
L	0010807	biological_process	regulation of synaptic vesicle priming
L	0015031	biological_process	protein transport
L	0016082	biological_process	synaptic vesicle priming
L	0016192	biological_process	vesicle-mediated transport
L	0019905	molecular_function	syntaxin binding
L	0030182	biological_process	neuron differentiation
L	0030425	cellular_component	dendrite
L	0031410	cellular_component	cytoplasmic vesicle
L	0031594	cellular_component	neuromuscular junction
L	0032781	biological_process	positive regulation of ATP-dependent activity
L	0032984	biological_process	protein-containing complex disassembly
L	0035249	biological_process	synaptic transmission, glutamatergic
L	0035494	biological_process	SNARE complex disassembly
L	0042599	cellular_component	lamellar body
L	0042734	cellular_component	presynaptic membrane
L	0043195	cellular_component	terminal bouton
L	0043204	cellular_component	perikaryon
L	0044877	molecular_function	protein-containing complex binding
L	0045176	biological_process	apical protein localization
L	0045211	cellular_component	postsynaptic membrane
L	0048787	cellular_component	presynaptic active zone membrane
L	0055093	biological_process	response to hyperoxia
L	0061025	biological_process	membrane fusion
L	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
L	0097060	cellular_component	synaptic membrane
L	0098793	cellular_component	presynapse
L	0098794	cellular_component	postsynapse
L	0098978	cellular_component	glutamatergic synapse

Functional Information from PROSITE/UniProt

site_id	PS00674
Number of Residues	19
Details	AAA AAA-protein family signature. IlVIgMTNrpdlIDeALl.R

Chain	Residue	Details
A	ILE367-ARG385

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	72
Details	Binding site: {"evidences":[{"source":"PubMed","id":"9731775","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9727495","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"9731775","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P46460","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine; by CDK16","evidences":[{"source":"UniProtKB","id":"P46460","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P46460","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P46459","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"UniProtKB","id":"P54920","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q9DB05","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P54920","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
A	LYS549	electrostatic stabiliser
A	THR550	electrostatic stabiliser
A	ASP603	electrostatic stabiliser
A	ASP604	proton acceptor
A	LYS631	electrostatic stabiliser
A	LYS708	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
B	LYS549	electrostatic stabiliser
B	THR550	electrostatic stabiliser
B	ASP603	electrostatic stabiliser
B	ASP604	proton acceptor
B	LYS631	electrostatic stabiliser
B	LYS708	electrostatic stabiliser

---

site_id	MCSA3
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
C	LYS549	electrostatic stabiliser
C	THR550	electrostatic stabiliser
C	ASP603	electrostatic stabiliser
C	ASP604	proton acceptor
C	LYS631	electrostatic stabiliser
C	LYS708	electrostatic stabiliser

---

site_id	MCSA4
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
D	LYS549	electrostatic stabiliser
D	THR550	electrostatic stabiliser
D	ASP603	electrostatic stabiliser
D	ASP604	proton acceptor
D	LYS631	electrostatic stabiliser
D	LYS708	electrostatic stabiliser

---

site_id	MCSA5
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
E	LYS549	electrostatic stabiliser
E	THR550	electrostatic stabiliser
E	ASP603	electrostatic stabiliser
E	ASP604	proton acceptor
E	LYS631	electrostatic stabiliser
E	LYS708	electrostatic stabiliser

---

site_id	MCSA6
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
F	LYS549	electrostatic stabiliser
F	THR550	electrostatic stabiliser
F	ASP603	electrostatic stabiliser
F	ASP604	proton acceptor
F	LYS631	electrostatic stabiliser
F	LYS708	electrostatic stabiliser

---