9P3D

cryo-EM structure of Vibrio effector VopV fragment bound to skeletal alpha F-actin

Functional Information from GO Data

Chain	GOid	namespace	contents
Q	0000166	molecular_function	nucleotide binding
Q	0000287	molecular_function	magnesium ion binding
Q	0001725	cellular_component	stress fiber
Q	0003785	molecular_function	actin monomer binding
Q	0005509	molecular_function	calcium ion binding
Q	0005515	molecular_function	protein binding
Q	0005523	molecular_function	tropomyosin binding
Q	0005524	molecular_function	ATP binding
Q	0005737	cellular_component	cytoplasm
Q	0005856	cellular_component	cytoskeleton
Q	0005865	cellular_component	striated muscle thin filament
Q	0005884	cellular_component	actin filament
Q	0010628	biological_process	positive regulation of gene expression
Q	0016787	molecular_function	hydrolase activity
Q	0019904	molecular_function	protein domain specific binding
Q	0030027	cellular_component	lamellipodium
Q	0030041	biological_process	actin filament polymerization
Q	0030175	cellular_component	filopodium
Q	0030240	biological_process	skeletal muscle thin filament assembly
Q	0031013	molecular_function	troponin I binding
Q	0031432	molecular_function	titin binding
Q	0031941	cellular_component	filamentous actin
Q	0032036	molecular_function	myosin heavy chain binding
Q	0032432	cellular_component	actin filament bundle
Q	0042802	molecular_function	identical protein binding
Q	0044297	cellular_component	cell body
Q	0048306	molecular_function	calcium-dependent protein binding
Q	0048741	biological_process	skeletal muscle fiber development
Q	0051017	biological_process	actin filament bundle assembly
Q	0090131	biological_process	mesenchyme migration
Q	0098723	cellular_component	skeletal muscle myofibril
Q	0140660	molecular_function	cytoskeletal motor activator activity
R	0000166	molecular_function	nucleotide binding
R	0000287	molecular_function	magnesium ion binding
R	0001725	cellular_component	stress fiber
R	0003785	molecular_function	actin monomer binding
R	0005509	molecular_function	calcium ion binding
R	0005515	molecular_function	protein binding
R	0005523	molecular_function	tropomyosin binding
R	0005524	molecular_function	ATP binding
R	0005737	cellular_component	cytoplasm
R	0005856	cellular_component	cytoskeleton
R	0005865	cellular_component	striated muscle thin filament
R	0005884	cellular_component	actin filament
R	0010628	biological_process	positive regulation of gene expression
R	0016787	molecular_function	hydrolase activity
R	0019904	molecular_function	protein domain specific binding
R	0030027	cellular_component	lamellipodium
R	0030041	biological_process	actin filament polymerization
R	0030175	cellular_component	filopodium
R	0030240	biological_process	skeletal muscle thin filament assembly
R	0031013	molecular_function	troponin I binding
R	0031432	molecular_function	titin binding
R	0031941	cellular_component	filamentous actin
R	0032036	molecular_function	myosin heavy chain binding
R	0032432	cellular_component	actin filament bundle
R	0042802	molecular_function	identical protein binding
R	0044297	cellular_component	cell body
R	0048306	molecular_function	calcium-dependent protein binding
R	0048741	biological_process	skeletal muscle fiber development
R	0051017	biological_process	actin filament bundle assembly
R	0090131	biological_process	mesenchyme migration
R	0098723	cellular_component	skeletal muscle myofibril
R	0140660	molecular_function	cytoskeletal motor activator activity
S	0000166	molecular_function	nucleotide binding
S	0000287	molecular_function	magnesium ion binding
S	0001725	cellular_component	stress fiber
S	0003785	molecular_function	actin monomer binding
S	0005509	molecular_function	calcium ion binding
S	0005515	molecular_function	protein binding
S	0005523	molecular_function	tropomyosin binding
S	0005524	molecular_function	ATP binding
S	0005737	cellular_component	cytoplasm
S	0005856	cellular_component	cytoskeleton
S	0005865	cellular_component	striated muscle thin filament
S	0005884	cellular_component	actin filament
S	0010628	biological_process	positive regulation of gene expression
S	0016787	molecular_function	hydrolase activity
S	0019904	molecular_function	protein domain specific binding
S	0030027	cellular_component	lamellipodium
S	0030041	biological_process	actin filament polymerization
S	0030175	cellular_component	filopodium
S	0030240	biological_process	skeletal muscle thin filament assembly
S	0031013	molecular_function	troponin I binding
S	0031432	molecular_function	titin binding
S	0031941	cellular_component	filamentous actin
S	0032036	molecular_function	myosin heavy chain binding
S	0032432	cellular_component	actin filament bundle
S	0042802	molecular_function	identical protein binding
S	0044297	cellular_component	cell body
S	0048306	molecular_function	calcium-dependent protein binding
S	0048741	biological_process	skeletal muscle fiber development
S	0051017	biological_process	actin filament bundle assembly
S	0090131	biological_process	mesenchyme migration
S	0098723	cellular_component	skeletal muscle myofibril
S	0140660	molecular_function	cytoskeletal motor activator activity
T	0000166	molecular_function	nucleotide binding
T	0000287	molecular_function	magnesium ion binding
T	0001725	cellular_component	stress fiber
T	0003785	molecular_function	actin monomer binding
T	0005509	molecular_function	calcium ion binding
T	0005515	molecular_function	protein binding
T	0005523	molecular_function	tropomyosin binding
T	0005524	molecular_function	ATP binding
T	0005737	cellular_component	cytoplasm
T	0005856	cellular_component	cytoskeleton
T	0005865	cellular_component	striated muscle thin filament
T	0005884	cellular_component	actin filament
T	0010628	biological_process	positive regulation of gene expression
T	0016787	molecular_function	hydrolase activity
T	0019904	molecular_function	protein domain specific binding
T	0030027	cellular_component	lamellipodium
T	0030041	biological_process	actin filament polymerization
T	0030175	cellular_component	filopodium
T	0030240	biological_process	skeletal muscle thin filament assembly
T	0031013	molecular_function	troponin I binding
T	0031432	molecular_function	titin binding
T	0031941	cellular_component	filamentous actin
T	0032036	molecular_function	myosin heavy chain binding
T	0032432	cellular_component	actin filament bundle
T	0042802	molecular_function	identical protein binding
T	0044297	cellular_component	cell body
T	0048306	molecular_function	calcium-dependent protein binding
T	0048741	biological_process	skeletal muscle fiber development
T	0051017	biological_process	actin filament bundle assembly
T	0090131	biological_process	mesenchyme migration
T	0098723	cellular_component	skeletal muscle myofibril
T	0140660	molecular_function	cytoskeletal motor activator activity
U	0000166	molecular_function	nucleotide binding
U	0000287	molecular_function	magnesium ion binding
U	0001725	cellular_component	stress fiber
U	0003785	molecular_function	actin monomer binding
U	0005509	molecular_function	calcium ion binding
U	0005515	molecular_function	protein binding
U	0005523	molecular_function	tropomyosin binding
U	0005524	molecular_function	ATP binding
U	0005737	cellular_component	cytoplasm
U	0005856	cellular_component	cytoskeleton
U	0005865	cellular_component	striated muscle thin filament
U	0005884	cellular_component	actin filament
U	0010628	biological_process	positive regulation of gene expression
U	0016787	molecular_function	hydrolase activity
U	0019904	molecular_function	protein domain specific binding
U	0030027	cellular_component	lamellipodium
U	0030041	biological_process	actin filament polymerization
U	0030175	cellular_component	filopodium
U	0030240	biological_process	skeletal muscle thin filament assembly
U	0031013	molecular_function	troponin I binding
U	0031432	molecular_function	titin binding
U	0031941	cellular_component	filamentous actin
U	0032036	molecular_function	myosin heavy chain binding
U	0032432	cellular_component	actin filament bundle
U	0042802	molecular_function	identical protein binding
U	0044297	cellular_component	cell body
U	0048306	molecular_function	calcium-dependent protein binding
U	0048741	biological_process	skeletal muscle fiber development
U	0051017	biological_process	actin filament bundle assembly
U	0090131	biological_process	mesenchyme migration
U	0098723	cellular_component	skeletal muscle myofibril
U	0140660	molecular_function	cytoskeletal motor activator activity
V	0000166	molecular_function	nucleotide binding
V	0000287	molecular_function	magnesium ion binding
V	0001725	cellular_component	stress fiber
V	0003785	molecular_function	actin monomer binding
V	0005509	molecular_function	calcium ion binding
V	0005515	molecular_function	protein binding
V	0005523	molecular_function	tropomyosin binding
V	0005524	molecular_function	ATP binding
V	0005737	cellular_component	cytoplasm
V	0005856	cellular_component	cytoskeleton
V	0005865	cellular_component	striated muscle thin filament
V	0005884	cellular_component	actin filament
V	0010628	biological_process	positive regulation of gene expression
V	0016787	molecular_function	hydrolase activity
V	0019904	molecular_function	protein domain specific binding
V	0030027	cellular_component	lamellipodium
V	0030041	biological_process	actin filament polymerization
V	0030175	cellular_component	filopodium
V	0030240	biological_process	skeletal muscle thin filament assembly
V	0031013	molecular_function	troponin I binding
V	0031432	molecular_function	titin binding
V	0031941	cellular_component	filamentous actin
V	0032036	molecular_function	myosin heavy chain binding
V	0032432	cellular_component	actin filament bundle
V	0042802	molecular_function	identical protein binding
V	0044297	cellular_component	cell body
V	0048306	molecular_function	calcium-dependent protein binding
V	0048741	biological_process	skeletal muscle fiber development
V	0051017	biological_process	actin filament bundle assembly
V	0090131	biological_process	mesenchyme migration
V	0098723	cellular_component	skeletal muscle myofibril
V	0140660	molecular_function	cytoskeletal motor activator activity
W	0000166	molecular_function	nucleotide binding
W	0000287	molecular_function	magnesium ion binding
W	0001725	cellular_component	stress fiber
W	0003785	molecular_function	actin monomer binding
W	0005509	molecular_function	calcium ion binding
W	0005515	molecular_function	protein binding
W	0005523	molecular_function	tropomyosin binding
W	0005524	molecular_function	ATP binding
W	0005737	cellular_component	cytoplasm
W	0005856	cellular_component	cytoskeleton
W	0005865	cellular_component	striated muscle thin filament
W	0005884	cellular_component	actin filament
W	0010628	biological_process	positive regulation of gene expression
W	0016787	molecular_function	hydrolase activity
W	0019904	molecular_function	protein domain specific binding
W	0030027	cellular_component	lamellipodium
W	0030041	biological_process	actin filament polymerization
W	0030175	cellular_component	filopodium
W	0030240	biological_process	skeletal muscle thin filament assembly
W	0031013	molecular_function	troponin I binding
W	0031432	molecular_function	titin binding
W	0031941	cellular_component	filamentous actin
W	0032036	molecular_function	myosin heavy chain binding
W	0032432	cellular_component	actin filament bundle
W	0042802	molecular_function	identical protein binding
W	0044297	cellular_component	cell body
W	0048306	molecular_function	calcium-dependent protein binding
W	0048741	biological_process	skeletal muscle fiber development
W	0051017	biological_process	actin filament bundle assembly
W	0090131	biological_process	mesenchyme migration
W	0098723	cellular_component	skeletal muscle myofibril
W	0140660	molecular_function	cytoskeletal motor activator activity
X	0000166	molecular_function	nucleotide binding
X	0000287	molecular_function	magnesium ion binding
X	0001725	cellular_component	stress fiber
X	0003785	molecular_function	actin monomer binding
X	0005509	molecular_function	calcium ion binding
X	0005515	molecular_function	protein binding
X	0005523	molecular_function	tropomyosin binding
X	0005524	molecular_function	ATP binding
X	0005737	cellular_component	cytoplasm
X	0005856	cellular_component	cytoskeleton
X	0005865	cellular_component	striated muscle thin filament
X	0005884	cellular_component	actin filament
X	0010628	biological_process	positive regulation of gene expression
X	0016787	molecular_function	hydrolase activity
X	0019904	molecular_function	protein domain specific binding
X	0030027	cellular_component	lamellipodium
X	0030041	biological_process	actin filament polymerization
X	0030175	cellular_component	filopodium
X	0030240	biological_process	skeletal muscle thin filament assembly
X	0031013	molecular_function	troponin I binding
X	0031432	molecular_function	titin binding
X	0031941	cellular_component	filamentous actin
X	0032036	molecular_function	myosin heavy chain binding
X	0032432	cellular_component	actin filament bundle
X	0042802	molecular_function	identical protein binding
X	0044297	cellular_component	cell body
X	0048306	molecular_function	calcium-dependent protein binding
X	0048741	biological_process	skeletal muscle fiber development
X	0051017	biological_process	actin filament bundle assembly
X	0090131	biological_process	mesenchyme migration
X	0098723	cellular_component	skeletal muscle myofibril
X	0140660	molecular_function	cytoskeletal motor activator activity
Y	0000166	molecular_function	nucleotide binding
Y	0000287	molecular_function	magnesium ion binding
Y	0001725	cellular_component	stress fiber
Y	0003785	molecular_function	actin monomer binding
Y	0005509	molecular_function	calcium ion binding
Y	0005515	molecular_function	protein binding
Y	0005523	molecular_function	tropomyosin binding
Y	0005524	molecular_function	ATP binding
Y	0005737	cellular_component	cytoplasm
Y	0005856	cellular_component	cytoskeleton
Y	0005865	cellular_component	striated muscle thin filament
Y	0005884	cellular_component	actin filament
Y	0010628	biological_process	positive regulation of gene expression
Y	0016787	molecular_function	hydrolase activity
Y	0019904	molecular_function	protein domain specific binding
Y	0030027	cellular_component	lamellipodium
Y	0030041	biological_process	actin filament polymerization
Y	0030175	cellular_component	filopodium
Y	0030240	biological_process	skeletal muscle thin filament assembly
Y	0031013	molecular_function	troponin I binding
Y	0031432	molecular_function	titin binding
Y	0031941	cellular_component	filamentous actin
Y	0032036	molecular_function	myosin heavy chain binding
Y	0032432	cellular_component	actin filament bundle
Y	0042802	molecular_function	identical protein binding
Y	0044297	cellular_component	cell body
Y	0048306	molecular_function	calcium-dependent protein binding
Y	0048741	biological_process	skeletal muscle fiber development
Y	0051017	biological_process	actin filament bundle assembly
Y	0090131	biological_process	mesenchyme migration
Y	0098723	cellular_component	skeletal muscle myofibril
Y	0140660	molecular_function	cytoskeletal motor activator activity
Z	0000166	molecular_function	nucleotide binding
Z	0000287	molecular_function	magnesium ion binding
Z	0001725	cellular_component	stress fiber
Z	0003785	molecular_function	actin monomer binding
Z	0005509	molecular_function	calcium ion binding
Z	0005515	molecular_function	protein binding
Z	0005523	molecular_function	tropomyosin binding
Z	0005524	molecular_function	ATP binding
Z	0005737	cellular_component	cytoplasm
Z	0005856	cellular_component	cytoskeleton
Z	0005865	cellular_component	striated muscle thin filament
Z	0005884	cellular_component	actin filament
Z	0010628	biological_process	positive regulation of gene expression
Z	0016787	molecular_function	hydrolase activity
Z	0019904	molecular_function	protein domain specific binding
Z	0030027	cellular_component	lamellipodium
Z	0030041	biological_process	actin filament polymerization
Z	0030175	cellular_component	filopodium
Z	0030240	biological_process	skeletal muscle thin filament assembly
Z	0031013	molecular_function	troponin I binding
Z	0031432	molecular_function	titin binding
Z	0031941	cellular_component	filamentous actin
Z	0032036	molecular_function	myosin heavy chain binding
Z	0032432	cellular_component	actin filament bundle
Z	0042802	molecular_function	identical protein binding
Z	0044297	cellular_component	cell body
Z	0048306	molecular_function	calcium-dependent protein binding
Z	0048741	biological_process	skeletal muscle fiber development
Z	0051017	biological_process	actin filament bundle assembly
Z	0090131	biological_process	mesenchyme migration
Z	0098723	cellular_component	skeletal muscle myofibril
Z	0140660	molecular_function	cytoskeletal motor activator activity
a	0000166	molecular_function	nucleotide binding
a	0000287	molecular_function	magnesium ion binding
a	0001725	cellular_component	stress fiber
a	0003785	molecular_function	actin monomer binding
a	0005509	molecular_function	calcium ion binding
a	0005515	molecular_function	protein binding
a	0005523	molecular_function	tropomyosin binding
a	0005524	molecular_function	ATP binding
a	0005737	cellular_component	cytoplasm
a	0005856	cellular_component	cytoskeleton
a	0005865	cellular_component	striated muscle thin filament
a	0005884	cellular_component	actin filament
a	0010628	biological_process	positive regulation of gene expression
a	0016787	molecular_function	hydrolase activity
a	0019904	molecular_function	protein domain specific binding
a	0030027	cellular_component	lamellipodium
a	0030041	biological_process	actin filament polymerization
a	0030175	cellular_component	filopodium
a	0030240	biological_process	skeletal muscle thin filament assembly
a	0031013	molecular_function	troponin I binding
a	0031432	molecular_function	titin binding
a	0031941	cellular_component	filamentous actin
a	0032036	molecular_function	myosin heavy chain binding
a	0032432	cellular_component	actin filament bundle
a	0042802	molecular_function	identical protein binding
a	0044297	cellular_component	cell body
a	0048306	molecular_function	calcium-dependent protein binding
a	0048741	biological_process	skeletal muscle fiber development
a	0051017	biological_process	actin filament bundle assembly
a	0090131	biological_process	mesenchyme migration
a	0098723	cellular_component	skeletal muscle myofibril
a	0140660	molecular_function	cytoskeletal motor activator activity

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
Q	TYR55-GLY65

---

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
Q	TRP358-GLU366

---

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
Q	LEU106-ARG118

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	275
Details	Region: {"description":"Interaction with alpha-actinin","evidences":[{"source":"PubMed","id":"8449927","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	Modified residue: {"description":"Methionine (R)-sulfoxide","evidences":[{"source":"UniProtKB","id":"P68134","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	11
Details	Modified residue: {"description":"N6-malonyllysine","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	11
Details	Modified residue: {"description":"Tele-methylhistidine","evidences":[{"source":"PubMed","id":"1150665","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16905096","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"213279","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"2395459","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"499690","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1ATN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1NWK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	11
Details	Modified residue: {"description":"N6-methyllysine","evidences":[{"source":"UniProtKB","id":"P68133","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	11
Details	Modified residue: {"description":"ADP-ribosylarginine; by SpvB","evidences":[{"source":"PubMed","id":"16905096","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---