9NHS

Cryo-EM structure of SIWI-piRNA-target(39-nt)-GTSF1-Maelstrom-Spindle-E complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0007283	biological_process	spermatogenesis
A	0016787	molecular_function	hydrolase activity
A	0030154	biological_process	cell differentiation
A	0030237	biological_process	female sex determination
A	0031047	biological_process	regulatory ncRNA-mediated gene silencing
A	0034584	molecular_function	piRNA binding
A	0034587	biological_process	piRNA processing
A	0046872	molecular_function	metal ion binding
A	0051321	biological_process	meiotic cell cycle
A	0140965	biological_process	secondary piRNA processing
A	0140991	biological_process	piRNA-mediated gene silencing by mRNA destabilization
A	1990923	cellular_component	PET complex
E	0008270	molecular_function	zinc ion binding
E	0046872	molecular_function	metal ion binding
F	0003677	molecular_function	DNA binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0007140	biological_process	male meiotic nuclear division
F	0007283	biological_process	spermatogenesis
F	0030154	biological_process	cell differentiation
F	0031047	biological_process	regulatory ncRNA-mediated gene silencing
F	0034587	biological_process	piRNA processing
F	0043186	cellular_component	P granule
F	0043565	molecular_function	sequence-specific DNA binding
F	0045892	biological_process	negative regulation of DNA-templated transcription
F	0051321	biological_process	meiotic cell cycle
G	0000166	molecular_function	nucleotide binding
G	0003723	molecular_function	RNA binding
G	0003724	molecular_function	RNA helicase activity
G	0004386	molecular_function	helicase activity
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0007283	biological_process	spermatogenesis
G	0016787	molecular_function	hydrolase activity
G	0030154	biological_process	cell differentiation
G	0031047	biological_process	regulatory ncRNA-mediated gene silencing
G	0051321	biological_process	meiotic cell cycle

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	110
Details	Domain: {"description":"PAZ","evidences":[{"source":"PROSITE-ProRule","id":"PRU00142","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	291
Details	Domain: {"description":"Piwi","evidences":[{"source":"PROSITE-ProRule","id":"PRU00150","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	76
Details	Region: {"description":"L1 region","evidences":[{"source":"PubMed","id":"27693359","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	99
Details	Region: {"description":"L2 region","evidences":[{"source":"PubMed","id":"27693359","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	130
Details	Region: {"description":"MID region","evidences":[{"source":"PubMed","id":"27693359","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI6
Number of Residues	4
Details	Active site: {"evidences":[{"source":"PubMed","id":"27693359","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5GUH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27693359","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5GUH","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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