9MKV
FnoCas12a bridge helix variant state 3
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Region: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Region: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 17 |
| Details | Region: {"description":"LKL, important for PAM recognition and DNA unwinding","evidences":[{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | Region: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 3 |
| Details | Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"26422227","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds DNA protospacer adjacent motif (PAM)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds PAM","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 2 |
| Details | Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds Target strand DNA; via amide nitrogen","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | Site: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 2 |
| Details | Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
