9M64

Structure of SPIN90 dimer-Arp2/3 complexes-nucleated actin filaments (Doublet Complex)

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0000166	molecular_function	nucleotide binding
a	0003779	molecular_function	actin binding
a	0005200	molecular_function	structural constituent of cytoskeleton
a	0005515	molecular_function	protein binding
a	0005524	molecular_function	ATP binding
a	0005634	cellular_component	nucleus
a	0005737	cellular_component	cytoplasm
a	0005829	cellular_component	cytosol
a	0005856	cellular_component	cytoskeleton
a	0005885	cellular_component	Arp2/3 protein complex
a	0010592	biological_process	positive regulation of lamellipodium assembly
a	0030030	biological_process	cell projection organization
a	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
a	0035861	cellular_component	site of double-strand break
a	0042995	cellular_component	cell projection
a	0045944	biological_process	positive regulation of transcription by RNA polymerase II
a	0051015	molecular_function	actin filament binding
a	0060271	biological_process	cilium assembly
A	0000166	molecular_function	nucleotide binding
A	0003779	molecular_function	actin binding
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005885	cellular_component	Arp2/3 protein complex
A	0010592	biological_process	positive regulation of lamellipodium assembly
A	0030030	biological_process	cell projection organization
A	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
A	0035861	cellular_component	site of double-strand break
A	0042995	cellular_component	cell projection
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0051015	molecular_function	actin filament binding
A	0060271	biological_process	cilium assembly
b	0000166	molecular_function	nucleotide binding
b	0003779	molecular_function	actin binding
b	0005200	molecular_function	structural constituent of cytoskeleton
b	0005515	molecular_function	protein binding
b	0005524	molecular_function	ATP binding
b	0005634	cellular_component	nucleus
b	0005737	cellular_component	cytoplasm
b	0005829	cellular_component	cytosol
b	0005856	cellular_component	cytoskeleton
b	0005885	cellular_component	Arp2/3 protein complex
b	0005938	cellular_component	cell cortex
b	0010592	biological_process	positive regulation of lamellipodium assembly
b	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
b	0035861	cellular_component	site of double-strand break
b	0042995	cellular_component	cell projection
b	0045944	biological_process	positive regulation of transcription by RNA polymerase II
b	0051015	molecular_function	actin filament binding
b	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
b	2001032	biological_process	regulation of double-strand break repair via nonhomologous end joining
B	0000166	molecular_function	nucleotide binding
B	0003779	molecular_function	actin binding
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0005885	cellular_component	Arp2/3 protein complex
B	0005938	cellular_component	cell cortex
B	0010592	biological_process	positive regulation of lamellipodium assembly
B	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
B	0035861	cellular_component	site of double-strand break
B	0042995	cellular_component	cell projection
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0051015	molecular_function	actin filament binding
B	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
B	2001032	biological_process	regulation of double-strand break repair via nonhomologous end joining
c	0003779	molecular_function	actin binding
c	0005634	cellular_component	nucleus
c	0005829	cellular_component	cytosol
c	0005856	cellular_component	cytoskeleton
c	0005885	cellular_component	Arp2/3 protein complex
c	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
c	0051015	molecular_function	actin filament binding
C	0003779	molecular_function	actin binding
C	0005634	cellular_component	nucleus
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005885	cellular_component	Arp2/3 protein complex
C	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
C	0051015	molecular_function	actin filament binding
d	0003779	molecular_function	actin binding
d	0005200	molecular_function	structural constituent of cytoskeleton
d	0005634	cellular_component	nucleus
d	0005654	cellular_component	nucleoplasm
d	0005768	cellular_component	endosome
d	0005829	cellular_component	cytosol
d	0005856	cellular_component	cytoskeleton
d	0005885	cellular_component	Arp2/3 protein complex
d	0005886	cellular_component	plasma membrane
d	0005925	cellular_component	focal adhesion
d	0010592	biological_process	positive regulation of lamellipodium assembly
d	0030027	cellular_component	lamellipodium
d	0030672	cellular_component	synaptic vesicle membrane
d	0031252	cellular_component	cell leading edge
d	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
d	0035861	cellular_component	site of double-strand break
d	0036195	cellular_component	muscle cell projection membrane
d	0042995	cellular_component	cell projection
d	0043005	cellular_component	neuron projection
d	0045202	cellular_component	synapse
d	0051015	molecular_function	actin filament binding
d	0098793	cellular_component	presynapse
d	0098794	cellular_component	postsynapse
d	0098978	cellular_component	glutamatergic synapse
D	0003779	molecular_function	actin binding
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005768	cellular_component	endosome
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0005885	cellular_component	Arp2/3 protein complex
D	0005886	cellular_component	plasma membrane
D	0005925	cellular_component	focal adhesion
D	0010592	biological_process	positive regulation of lamellipodium assembly
D	0030027	cellular_component	lamellipodium
D	0030672	cellular_component	synaptic vesicle membrane
D	0031252	cellular_component	cell leading edge
D	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
D	0035861	cellular_component	site of double-strand break
D	0036195	cellular_component	muscle cell projection membrane
D	0042995	cellular_component	cell projection
D	0043005	cellular_component	neuron projection
D	0045202	cellular_component	synapse
D	0051015	molecular_function	actin filament binding
D	0098793	cellular_component	presynapse
D	0098794	cellular_component	postsynapse
D	0098978	cellular_component	glutamatergic synapse
e	0003779	molecular_function	actin binding
e	0005515	molecular_function	protein binding
e	0005634	cellular_component	nucleus
e	0005737	cellular_component	cytoplasm
e	0005829	cellular_component	cytosol
e	0005856	cellular_component	cytoskeleton
e	0005885	cellular_component	Arp2/3 protein complex
e	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
e	0035861	cellular_component	site of double-strand break
e	0042995	cellular_component	cell projection
e	0051015	molecular_function	actin filament binding
E	0003779	molecular_function	actin binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005885	cellular_component	Arp2/3 protein complex
E	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
E	0035861	cellular_component	site of double-strand break
E	0042995	cellular_component	cell projection
E	0051015	molecular_function	actin filament binding
f	0003779	molecular_function	actin binding
f	0005634	cellular_component	nucleus
f	0005829	cellular_component	cytosol
f	0005856	cellular_component	cytoskeleton
f	0005885	cellular_component	Arp2/3 protein complex
f	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
f	0035861	cellular_component	site of double-strand break
f	0042995	cellular_component	cell projection
f	0051015	molecular_function	actin filament binding
F	0003779	molecular_function	actin binding
F	0005634	cellular_component	nucleus
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005885	cellular_component	Arp2/3 protein complex
F	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
F	0035861	cellular_component	site of double-strand break
F	0042995	cellular_component	cell projection
F	0051015	molecular_function	actin filament binding
g	0003779	molecular_function	actin binding
g	0005634	cellular_component	nucleus
g	0005829	cellular_component	cytosol
g	0005856	cellular_component	cytoskeleton
g	0005885	cellular_component	Arp2/3 protein complex
g	0016477	biological_process	cell migration
g	0030674	molecular_function	protein-macromolecule adaptor activity
g	0030863	cellular_component	cortical cytoskeleton
g	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
g	0035861	cellular_component	site of double-strand break
g	0042995	cellular_component	cell projection
G	0003779	molecular_function	actin binding
G	0005634	cellular_component	nucleus
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005885	cellular_component	Arp2/3 protein complex
G	0016477	biological_process	cell migration
G	0030674	molecular_function	protein-macromolecule adaptor activity
G	0030863	cellular_component	cortical cytoskeleton
G	0034314	biological_process	Arp2/3 complex-mediated actin nucleation
G	0035861	cellular_component	site of double-strand break
G	0042995	cellular_component	cell projection
i	0000166	molecular_function	nucleotide binding
i	0001725	cellular_component	stress fiber
i	0005200	molecular_function	structural constituent of cytoskeleton
i	0005515	molecular_function	protein binding
i	0005524	molecular_function	ATP binding
i	0005856	cellular_component	cytoskeleton
i	0005865	cellular_component	striated muscle thin filament
i	0005884	cellular_component	actin filament
i	0015629	cellular_component	actin cytoskeleton
i	0016787	molecular_function	hydrolase activity
i	0030240	biological_process	skeletal muscle thin filament assembly
i	0048741	biological_process	skeletal muscle fiber development
I	0000166	molecular_function	nucleotide binding
I	0001725	cellular_component	stress fiber
I	0005200	molecular_function	structural constituent of cytoskeleton
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005856	cellular_component	cytoskeleton
I	0005865	cellular_component	striated muscle thin filament
I	0005884	cellular_component	actin filament
I	0015629	cellular_component	actin cytoskeleton
I	0016787	molecular_function	hydrolase activity
I	0030240	biological_process	skeletal muscle thin filament assembly
I	0048741	biological_process	skeletal muscle fiber development
j	0000166	molecular_function	nucleotide binding
j	0001725	cellular_component	stress fiber
j	0005200	molecular_function	structural constituent of cytoskeleton
j	0005515	molecular_function	protein binding
j	0005524	molecular_function	ATP binding
j	0005856	cellular_component	cytoskeleton
j	0005865	cellular_component	striated muscle thin filament
j	0005884	cellular_component	actin filament
j	0015629	cellular_component	actin cytoskeleton
j	0016787	molecular_function	hydrolase activity
j	0030240	biological_process	skeletal muscle thin filament assembly
j	0048741	biological_process	skeletal muscle fiber development
J	0000166	molecular_function	nucleotide binding
J	0001725	cellular_component	stress fiber
J	0005200	molecular_function	structural constituent of cytoskeleton
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005856	cellular_component	cytoskeleton
J	0005865	cellular_component	striated muscle thin filament
J	0005884	cellular_component	actin filament
J	0015629	cellular_component	actin cytoskeleton
J	0016787	molecular_function	hydrolase activity
J	0030240	biological_process	skeletal muscle thin filament assembly
J	0048741	biological_process	skeletal muscle fiber development
k	0000166	molecular_function	nucleotide binding
k	0001725	cellular_component	stress fiber
k	0005200	molecular_function	structural constituent of cytoskeleton
k	0005515	molecular_function	protein binding
k	0005524	molecular_function	ATP binding
k	0005856	cellular_component	cytoskeleton
k	0005865	cellular_component	striated muscle thin filament
k	0005884	cellular_component	actin filament
k	0015629	cellular_component	actin cytoskeleton
k	0016787	molecular_function	hydrolase activity
k	0030240	biological_process	skeletal muscle thin filament assembly
k	0048741	biological_process	skeletal muscle fiber development
K	0000166	molecular_function	nucleotide binding
K	0001725	cellular_component	stress fiber
K	0005200	molecular_function	structural constituent of cytoskeleton
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005856	cellular_component	cytoskeleton
K	0005865	cellular_component	striated muscle thin filament
K	0005884	cellular_component	actin filament
K	0015629	cellular_component	actin cytoskeleton
K	0016787	molecular_function	hydrolase activity
K	0030240	biological_process	skeletal muscle thin filament assembly
K	0048741	biological_process	skeletal muscle fiber development
l	0000166	molecular_function	nucleotide binding
l	0001725	cellular_component	stress fiber
l	0005200	molecular_function	structural constituent of cytoskeleton
l	0005515	molecular_function	protein binding
l	0005524	molecular_function	ATP binding
l	0005856	cellular_component	cytoskeleton
l	0005865	cellular_component	striated muscle thin filament
l	0005884	cellular_component	actin filament
l	0015629	cellular_component	actin cytoskeleton
l	0016787	molecular_function	hydrolase activity
l	0030240	biological_process	skeletal muscle thin filament assembly
l	0048741	biological_process	skeletal muscle fiber development
L	0000166	molecular_function	nucleotide binding
L	0001725	cellular_component	stress fiber
L	0005200	molecular_function	structural constituent of cytoskeleton
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005856	cellular_component	cytoskeleton
L	0005865	cellular_component	striated muscle thin filament
L	0005884	cellular_component	actin filament
L	0015629	cellular_component	actin cytoskeleton
L	0016787	molecular_function	hydrolase activity
L	0030240	biological_process	skeletal muscle thin filament assembly
L	0048741	biological_process	skeletal muscle fiber development

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
I	TYR53-GLY63

---

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
I	TRP356-GLU364

---

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
I	LEU104-ARG116
A	LEU111-ARG123
B	LEU108-ARG120

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P61158","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15505213","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17499050","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"UniProtKB","id":"P61160","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P61160","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	78
Details	Repeat: {"description":"WD 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	78
Details	Repeat: {"description":"WD 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	82
Details	Repeat: {"description":"WD 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	78
Details	Repeat: {"description":"WD 4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	76
Details	Repeat: {"description":"WD 5"}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"O15144","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"O15145","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"O15145","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"UniProtKB","id":"O15145","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"N-acetylthreonine","evidences":[{"source":"UniProtKB","id":"P59998","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	16
Details	Modified residue: {"description":"Methionine (R)-sulfoxide","evidences":[{"source":"UniProtKB","id":"P68134","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	8
Details	Modified residue: {"description":"Tele-methylhistidine","evidences":[{"source":"PubMed","id":"12356759","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1MDU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	8
Details	Modified residue: {"description":"N6-methyllysine","evidences":[{"source":"UniProtKB","id":"P68133","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---