9LY3
A Cryo-EM structure of LA-PTH-PTH1R-V2RT-Beta-arrestin1 complex (state 2 conformation)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005179 | molecular_function | hormone activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0030282 | biological_process | bone mineralization |
| B | 0000139 | cellular_component | Golgi membrane |
| B | 0000785 | cellular_component | chromatin |
| B | 0001664 | molecular_function | G protein-coupled receptor binding |
| B | 0001678 | biological_process | intracellular glucose homeostasis |
| B | 0001934 | biological_process | positive regulation of protein phosphorylation |
| B | 0002029 | biological_process | desensitization of G protein-coupled receptor signaling pathway |
| B | 0002031 | biological_process | G protein-coupled receptor internalization |
| B | 0002092 | biological_process | positive regulation of receptor internalization |
| B | 0003713 | molecular_function | transcription coactivator activity |
| B | 0004402 | molecular_function | histone acetyltransferase activity |
| B | 0004857 | molecular_function | enzyme inhibitor activity |
| B | 0004869 | molecular_function | cysteine-type endopeptidase inhibitor activity |
| B | 0005096 | molecular_function | GTPase activator activity |
| B | 0005159 | molecular_function | insulin-like growth factor receptor binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005765 | cellular_component | lysosomal membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0005905 | cellular_component | clathrin-coated pit |
| B | 0005929 | cellular_component | cilium |
| B | 0006357 | biological_process | regulation of transcription by RNA polymerase II |
| B | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| B | 0007165 | biological_process | signal transduction |
| B | 0007166 | biological_process | cell surface receptor signaling pathway |
| B | 0007600 | biological_process | sensory perception |
| B | 0016567 | biological_process | protein ubiquitination |
| B | 0019899 | molecular_function | enzyme binding |
| B | 0030659 | cellular_component | cytoplasmic vesicle membrane |
| B | 0030666 | cellular_component | endocytic vesicle membrane |
| B | 0030674 | molecular_function | protein-macromolecule adaptor activity |
| B | 0031143 | cellular_component | pseudopodium |
| B | 0031410 | cellular_component | cytoplasmic vesicle |
| B | 0031434 | molecular_function | mitogen-activated protein kinase kinase binding |
| B | 0031625 | molecular_function | ubiquitin protein ligase binding |
| B | 0031701 | molecular_function | angiotensin receptor binding |
| B | 0032715 | biological_process | negative regulation of interleukin-6 production |
| B | 0032717 | biological_process | negative regulation of interleukin-8 production |
| B | 0035025 | biological_process | positive regulation of Rho protein signal transduction |
| B | 0035721 | biological_process | intraciliary retrograde transport |
| B | 0035774 | biological_process | positive regulation of insulin secretion involved in cellular response to glucose stimulus |
| B | 0042981 | biological_process | regulation of apoptotic process |
| B | 0043124 | biological_process | negative regulation of canonical NF-kappaB signal transduction |
| B | 0043149 | biological_process | stress fiber assembly |
| B | 0043161 | biological_process | proteasome-mediated ubiquitin-dependent protein catabolic process |
| B | 0045746 | biological_process | negative regulation of Notch signaling pathway |
| B | 0045880 | biological_process | positive regulation of smoothened signaling pathway |
| B | 0045944 | biological_process | positive regulation of transcription by RNA polymerase II |
| B | 0060090 | molecular_function | molecular adaptor activity |
| B | 0070374 | biological_process | positive regulation of ERK1 and ERK2 cascade |
| B | 0097499 | biological_process | protein localization to non-motile cilium |
| B | 1902533 | biological_process | positive regulation of intracellular signal transduction |
| B | 1903568 | biological_process | negative regulation of protein localization to ciliary membrane |
| B | 1990763 | molecular_function | arrestin family protein binding |
| R | 0004888 | molecular_function | transmembrane signaling receptor activity |
| R | 0004930 | molecular_function | G protein-coupled receptor activity |
| R | 0004991 | molecular_function | parathyroid hormone receptor activity |
| R | 0007166 | biological_process | cell surface receptor signaling pathway |
| R | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| R | 0016020 | cellular_component | membrane |
Functional Information from PROSITE/UniProt
| site_id | PS00290 |
| Number of Residues | 7 |
| Details | IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YACEVTH |
| Chain | Residue | Details |
| E | TYR193-HIS199 | |
| C | TYR204-HIS210 |
| site_id | PS00295 |
| Number of Residues | 19 |
| Details | ARRESTINS Arrestins signature. FRYGrEDlDVLGLtFrKDL |
| Chain | Residue | Details |
| B | PHE61-LEU79 |
| site_id | PS00649 |
| Number of Residues | 25 |
| Details | G_PROTEIN_RECEP_F2_1 G-protein coupled receptors family 2 signature 1. ClpeWDhil.CWplGapgevvavpCP |
| Chain | Residue | Details |
| R | CYS108-PRO132 |
| site_id | PS00650 |
| Number of Residues | 16 |
| Details | G_PROTEIN_RECEP_F2_2 G-protein coupled receptors family 2 signature 2. QGFFVaIIYCFcNgeV |
| Chain | Residue | Details |
| R | GLN451-VAL466 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 41 |
| Details | Region: {"description":"Interaction with CHRM2","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"Q8BWG8","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"37209686","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8GOC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8I10","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 24 |
| Details | Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 7 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 21 |
| Details | Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 24 |
| Details | Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 21 |
| Details | Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 21 |
| Details | Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 24 |
| Details | Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 19 |
| Details | Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 27 |
| Details | Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"30975883","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NBF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NBI","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 4 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
