9J79

Cryo-EM structure of CRL2-FEM1B bound with TOM20(tetramer)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000151	cellular_component	ubiquitin ligase complex
A	0005123	molecular_function	death receptor binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006915	biological_process	apoptotic process
A	0016567	biological_process	protein ubiquitination
A	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0046872	molecular_function	metal ion binding
A	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
A	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
A	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
A	2000001	biological_process	regulation of DNA damage checkpoint
B	0005742	cellular_component	mitochondrial outer membrane translocase complex
B	0006605	biological_process	protein targeting
B	0006886	biological_process	intracellular protein transport
B	0030150	biological_process	protein import into mitochondrial matrix
C	0005742	cellular_component	mitochondrial outer membrane translocase complex
C	0006605	biological_process	protein targeting
C	0006886	biological_process	intracellular protein transport
C	0030150	biological_process	protein import into mitochondrial matrix
E	0000082	biological_process	G1/S transition of mitotic cell cycle
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005730	cellular_component	nucleolus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0010498	biological_process	proteasomal protein catabolic process
E	0016567	biological_process	protein ubiquitination
E	0019005	cellular_component	SCF ubiquitin ligase complex
E	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
E	0031461	cellular_component	cullin-RING ubiquitin ligase complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0031981	cellular_component	nuclear lumen
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0072344	biological_process	rescue of stalled ribosome
E	0097193	biological_process	intrinsic apoptotic signaling pathway
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	0160072	molecular_function	ubiquitin ligase complex scaffold activity
E	0160276	biological_process	negative regulation of beige fat cell differentiation
E	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
E	2000104	biological_process	negative regulation of DNA-templated DNA replication
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0005742	cellular_component	mitochondrial outer membrane translocase complex
G	0006605	biological_process	protein targeting
G	0006886	biological_process	intracellular protein transport
G	0030150	biological_process	protein import into mitochondrial matrix
H	0000122	biological_process	negative regulation of transcription by RNA polymerase II
H	0000151	cellular_component	ubiquitin ligase complex
H	0001222	molecular_function	transcription corepressor binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006351	biological_process	DNA-templated transcription
H	0006367	biological_process	transcription initiation at RNA polymerase II promoter
H	0006368	biological_process	transcription elongation by RNA polymerase II
H	0016567	biological_process	protein ubiquitination
H	0030891	cellular_component	VCB complex
H	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
H	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
H	0031625	molecular_function	ubiquitin protein ligase binding
H	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
H	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
H	0065003	biological_process	protein-containing complex assembly
H	0070449	cellular_component	elongin complex
H	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
H	0140958	biological_process	target-directed miRNA degradation
H	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
H	2000104	biological_process	negative regulation of DNA-templated DNA replication
I	0008270	molecular_function	zinc ion binding
J	0000151	cellular_component	ubiquitin ligase complex
J	0005123	molecular_function	death receptor binding
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005739	cellular_component	mitochondrion
J	0005829	cellular_component	cytosol
J	0006915	biological_process	apoptotic process
J	0016567	biological_process	protein ubiquitination
J	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0046872	molecular_function	metal ion binding
J	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
J	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
J	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
J	2000001	biological_process	regulation of DNA damage checkpoint
K	0000082	biological_process	G1/S transition of mitotic cell cycle
K	0000122	biological_process	negative regulation of transcription by RNA polymerase II
K	0004842	molecular_function	ubiquitin-protein transferase activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005730	cellular_component	nucleolus
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006511	biological_process	ubiquitin-dependent protein catabolic process
K	0010498	biological_process	proteasomal protein catabolic process
K	0016567	biological_process	protein ubiquitination
K	0019005	cellular_component	SCF ubiquitin ligase complex
K	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
K	0031461	cellular_component	cullin-RING ubiquitin ligase complex
K	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
K	0031625	molecular_function	ubiquitin protein ligase binding
K	0031981	cellular_component	nuclear lumen
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0072344	biological_process	rescue of stalled ribosome
K	0097193	biological_process	intrinsic apoptotic signaling pathway
K	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
K	0160072	molecular_function	ubiquitin ligase complex scaffold activity
K	0160276	biological_process	negative regulation of beige fat cell differentiation
K	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
K	2000104	biological_process	negative regulation of DNA-templated DNA replication
L	0006511	biological_process	ubiquitin-dependent protein catabolic process
M	0000122	biological_process	negative regulation of transcription by RNA polymerase II
M	0000151	cellular_component	ubiquitin ligase complex
M	0001222	molecular_function	transcription corepressor binding
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0006351	biological_process	DNA-templated transcription
M	0006367	biological_process	transcription initiation at RNA polymerase II promoter
M	0006368	biological_process	transcription elongation by RNA polymerase II
M	0016567	biological_process	protein ubiquitination
M	0030891	cellular_component	VCB complex
M	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
M	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
M	0031625	molecular_function	ubiquitin protein ligase binding
M	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
M	0065003	biological_process	protein-containing complex assembly
M	0070449	cellular_component	elongin complex
M	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
M	0140958	biological_process	target-directed miRNA degradation
M	1990116	biological_process	ribosome-associated ubiquitin-dependent protein catabolic process
M	2000104	biological_process	negative regulation of DNA-templated DNA replication
N	0008270	molecular_function	zinc ion binding
O	0000151	cellular_component	ubiquitin ligase complex
O	0005123	molecular_function	death receptor binding
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005739	cellular_component	mitochondrion
O	0005829	cellular_component	cytosol
O	0006915	biological_process	apoptotic process
O	0016567	biological_process	protein ubiquitination
O	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
O	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0046872	molecular_function	metal ion binding
O	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
O	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
O	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
O	2000001	biological_process	regulation of DNA damage checkpoint
P	0000151	cellular_component	ubiquitin ligase complex
P	0005123	molecular_function	death receptor binding
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005739	cellular_component	mitochondrion
P	0005829	cellular_component	cytosol
P	0006915	biological_process	apoptotic process
P	0016567	biological_process	protein ubiquitination
P	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0046872	molecular_function	metal ion binding
P	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
P	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
P	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
P	2000001	biological_process	regulation of DNA damage checkpoint
S	0005742	cellular_component	mitochondrial outer membrane translocase complex
S	0006605	biological_process	protein targeting
S	0006886	biological_process	intracellular protein transport
S	0030150	biological_process	protein import into mitochondrial matrix

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
E	ILE718-ALA745

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	120
Details	Domain: {"description":"Cullin neddylation","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)","evidences":[{"source":"PubMed","id":"10092517","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10597293","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11961546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38605244","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LDJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1LDK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1U6G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HYE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DPL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DQV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RTR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4F52","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4P5O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q7H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8RHZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11961546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38605244","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LDJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1LDK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1U6G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HYE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DPL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DQV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RTR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4F52","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4P5O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7Z8B","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q7H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8RHZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11961546","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38605244","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1LDJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1LDK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1U6G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2HYE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3DQV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RTR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4F52","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4P5O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q7H","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8RHZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	45
Details	Zinc finger: {"description":"RING-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00175","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	116
Details	Repeat: {"description":"ANK 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	116
Details	Repeat: {"description":"ANK 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	116
Details	Repeat: {"description":"ANK 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	116
Details	Repeat: {"description":"ANK 4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	116
Details	Repeat: {"description":"ANK 5"}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	120
Details	Repeat: {"description":"ANK 6"}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	132
Details	Repeat: {"description":"TPR"}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	176
Details	Repeat: {"description":"ANK 7"}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	111
Details	Repeat: {"description":"ANK 8"}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q9Z2G0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	4
Details	Site: {"description":"Cleavage; by a caspase-3-like protease","evidences":[{"source":"PubMed","id":"10542291","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"24896179","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---