9J6X

Cryo-EM structure of the rice isoamylase ISA1-ISA2 heterocomplex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005515	molecular_function	protein binding
A	0005975	biological_process	carbohydrate metabolic process
A	0005983	biological_process	starch catabolic process
A	0009507	cellular_component	chloroplast
A	0010021	biological_process	amylopectin biosynthetic process
A	0010368	cellular_component	chloroplast isoamylase complex
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0019156	molecular_function	isoamylase activity
A	0019252	biological_process	starch biosynthetic process
A	0043033	cellular_component	isoamylase complex
B	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
B	0005515	molecular_function	protein binding
B	0005975	biological_process	carbohydrate metabolic process
B	0005983	biological_process	starch catabolic process
B	0009507	cellular_component	chloroplast
B	0010021	biological_process	amylopectin biosynthetic process
B	0010368	cellular_component	chloroplast isoamylase complex
B	0016787	molecular_function	hydrolase activity
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0019156	molecular_function	isoamylase activity
B	0019252	biological_process	starch biosynthetic process
B	0043033	cellular_component	isoamylase complex
C	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
C	0005975	biological_process	carbohydrate metabolic process
C	0005983	biological_process	starch catabolic process
C	0009507	cellular_component	chloroplast
C	0010021	biological_process	amylopectin biosynthetic process
C	0016787	molecular_function	hydrolase activity
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0019156	molecular_function	isoamylase activity
C	0019252	biological_process	starch biosynthetic process
C	0043033	cellular_component	isoamylase complex

Functional Information from PROSITE/UniProt

site_id	PS00187
Number of Residues	20
Details	TPP_ENZYMES Thiamine pyrophosphate enzymes signature. VGvgpgsPaplgMsIsGDGA

Chain	Residue	Details
C	VAL143-ALA162

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P04746","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Active site: {"description":"Proton donor","evidences":[{"source":"UniProtKB","id":"P04746","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"UniProtKB","id":"P04746","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---