9HNP
Cryo-EM structure of the glucose-specific PTS transporter IICB from E. coli in an intermediate state
Functional Information from PROSITE/UniProt
| site_id | PS01035 |
| Number of Residues | 18 |
| Details | PTS_EIIB_TYPE_1_CYS PTS EIIB domains cysteine phosphorylation site signature. NItnldaCiTRLrVsVaD |
| Chain | Residue | Details |
| B | ASN414-ASP431 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 166 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000255 |
| Chain | Residue | Details |
| B | MET1-LYS14 | |
| A | GLU301-PRO309 | |
| B | THR72-ALA79 | |
| B | ASN133-ARG151 | |
| B | HIS212-LYS249 | |
| B | GLU301-PRO309 | |
| A | MET1-LYS14 | |
| A | THR72-ALA79 | |
| A | ASN133-ARG151 | |
| A | HIS212-LYS249 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 400 |
| Details | TRANSMEM: Helical => ECO:0000255|PROSITE-ProRule:PRU00426 |
| Chain | Residue | Details |
| B | SER15-ALA35 | |
| B | LEU356-ILE376 | |
| A | SER15-ALA35 | |
| A | ALA51-PHE71 | |
| A | LEU80-VAL100 | |
| A | HIS112-PHE132 | |
| A | PHE152-TRP172 | |
| A | PRO191-HIS211 | |
| A | LEU250-HIS270 | |
| A | VAL280-ILE300 | |
| A | ILE310-GLY330 | |
| B | ALA51-PHE71 | |
| A | LEU356-ILE376 | |
| B | LEU80-VAL100 | |
| B | HIS112-PHE132 | |
| B | PHE152-TRP172 | |
| B | PRO191-HIS211 | |
| B | LEU250-HIS270 | |
| B | VAL280-ILE300 | |
| B | ILE310-GLY330 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 146 |
| Details | TOPO_DOM: Periplasmic => ECO:0000255 |
| Chain | Residue | Details |
| B | ASN36-GLU50 | |
| A | MET331-TRP355 | |
| B | LEU101-LYS111 | |
| B | PRO173-ASN190 | |
| B | SER271-LYS279 | |
| B | MET331-TRP355 | |
| A | ASN36-GLU50 | |
| A | LEU101-LYS111 | |
| A | PRO173-ASN190 | |
| A | SER271-LYS279 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 200 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:15919996 |
| Chain | Residue | Details |
| B | LYS377-HIS477 | |
| A | LYS377-HIS477 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | ACT_SITE: Phosphocysteine intermediate; for EIIB activity => ECO:0000255|PROSITE-ProRule:PRU00421, ECO:0000305|PubMed:12716891, ECO:0000305|PubMed:18319344, ECO:0000305|PubMed:3129430 |
| Chain | Residue | Details |
| B | CYS421 | |
| A | CYS421 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphocysteine => ECO:0000269|PubMed:8505292 |
| Chain | Residue | Details |
| B | CYS421 | |
| A | CYS421 |
