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9FJU

Structure of the DNase I- and phalloidin-bound pointed end of F-actin (conformer 1)

Functional Information from GO Data
ChainGOidnamespacecontents
A0000166molecular_functionnucleotide binding
A0000786cellular_componentnucleosome
A0001738biological_processmorphogenesis of a polarized epithelium
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005634cellular_componentnucleus
A0005737cellular_componentcytoplasm
A0005829cellular_componentcytosol
A0005856cellular_componentcytoskeleton
A0005884cellular_componentactin filament
A0005886cellular_componentplasma membrane
A0005903cellular_componentbrush border
A0005911cellular_componentcell-cell junction
A0005912cellular_componentadherens junction
A0005925cellular_componentfocal adhesion
A0007163biological_processestablishment or maintenance of cell polarity
A0007409biological_processaxonogenesis
A0015629cellular_componentactin cytoskeleton
A0016020cellular_componentmembrane
A0016787molecular_functionhydrolase activity
A0016887molecular_functionATP hydrolysis activity
A0019894molecular_functionkinesin binding
A0019901molecular_functionprotein kinase binding
A0030027cellular_componentlamellipodium
A0030235molecular_functionnitric-oxide synthase regulator activity
A0030424cellular_componentaxon
A0030863cellular_componentcortical cytoskeleton
A0030957molecular_functionTat protein binding
A0032991cellular_componentprotein-containing complex
A0034333biological_processadherens junction assembly
A0035267cellular_componentNuA4 histone acetyltransferase complex
A0036464cellular_componentcytoplasmic ribonucleoprotein granule
A0042802molecular_functionidentical protein binding
A0043296cellular_componentapical junction complex
A0044305cellular_componentcalyx of Held
A0045176biological_processapical protein localization
A0045202cellular_componentsynapse
A0048870biological_processcell motility
A0050998molecular_functionnitric-oxide synthase binding
A0051621biological_processregulation of norepinephrine uptake
A0051726biological_processregulation of cell cycle
A0070160cellular_componenttight junction
A0071896biological_processprotein localization to adherens junction
A0072749biological_processcellular response to cytochalasin B
A0097433cellular_componentdense body
A0098685cellular_componentSchaffer collateral - CA1 synapse
A0098871cellular_componentpostsynaptic actin cytoskeleton
A0098973molecular_functionstructural constituent of postsynaptic actin cytoskeleton
A0098974biological_processpostsynaptic actin cytoskeleton organization
A0098978cellular_componentglutamatergic synapse
A0141108molecular_functiontransporter regulator activity
A0150111biological_processregulation of transepithelial transport
A1900242biological_processregulation of synaptic vesicle endocytosis
A1903076biological_processregulation of protein localization to plasma membrane
A1905168biological_processpositive regulation of double-strand break repair via homologous recombination
A1990904cellular_componentribonucleoprotein complex
B0000166molecular_functionnucleotide binding
B0000786cellular_componentnucleosome
B0001738biological_processmorphogenesis of a polarized epithelium
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005884cellular_componentactin filament
B0005886cellular_componentplasma membrane
B0005903cellular_componentbrush border
B0005911cellular_componentcell-cell junction
B0005912cellular_componentadherens junction
B0005925cellular_componentfocal adhesion
B0007163biological_processestablishment or maintenance of cell polarity
B0007409biological_processaxonogenesis
B0015629cellular_componentactin cytoskeleton
B0016020cellular_componentmembrane
B0016787molecular_functionhydrolase activity
B0016887molecular_functionATP hydrolysis activity
B0019894molecular_functionkinesin binding
B0019901molecular_functionprotein kinase binding
B0030027cellular_componentlamellipodium
B0030235molecular_functionnitric-oxide synthase regulator activity
B0030424cellular_componentaxon
B0030863cellular_componentcortical cytoskeleton
B0030957molecular_functionTat protein binding
B0032991cellular_componentprotein-containing complex
B0034333biological_processadherens junction assembly
B0035267cellular_componentNuA4 histone acetyltransferase complex
B0036464cellular_componentcytoplasmic ribonucleoprotein granule
B0042802molecular_functionidentical protein binding
B0043296cellular_componentapical junction complex
B0044305cellular_componentcalyx of Held
B0045176biological_processapical protein localization
B0045202cellular_componentsynapse
B0048870biological_processcell motility
B0050998molecular_functionnitric-oxide synthase binding
B0051621biological_processregulation of norepinephrine uptake
B0051726biological_processregulation of cell cycle
B0070160cellular_componenttight junction
B0071896biological_processprotein localization to adherens junction
B0072749biological_processcellular response to cytochalasin B
B0097433cellular_componentdense body
B0098685cellular_componentSchaffer collateral - CA1 synapse
B0098871cellular_componentpostsynaptic actin cytoskeleton
B0098973molecular_functionstructural constituent of postsynaptic actin cytoskeleton
B0098974biological_processpostsynaptic actin cytoskeleton organization
B0098978cellular_componentglutamatergic synapse
B0141108molecular_functiontransporter regulator activity
B0150111biological_processregulation of transepithelial transport
B1900242biological_processregulation of synaptic vesicle endocytosis
B1903076biological_processregulation of protein localization to plasma membrane
B1905168biological_processpositive regulation of double-strand break repair via homologous recombination
B1990904cellular_componentribonucleoprotein complex
C0000166molecular_functionnucleotide binding
C0000786cellular_componentnucleosome
C0001738biological_processmorphogenesis of a polarized epithelium
C0005515molecular_functionprotein binding
C0005524molecular_functionATP binding
C0005634cellular_componentnucleus
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0005856cellular_componentcytoskeleton
C0005884cellular_componentactin filament
C0005886cellular_componentplasma membrane
C0005903cellular_componentbrush border
C0005911cellular_componentcell-cell junction
C0005912cellular_componentadherens junction
C0005925cellular_componentfocal adhesion
C0007163biological_processestablishment or maintenance of cell polarity
C0007409biological_processaxonogenesis
C0015629cellular_componentactin cytoskeleton
C0016020cellular_componentmembrane
C0016787molecular_functionhydrolase activity
C0016887molecular_functionATP hydrolysis activity
C0019894molecular_functionkinesin binding
C0019901molecular_functionprotein kinase binding
C0030027cellular_componentlamellipodium
C0030235molecular_functionnitric-oxide synthase regulator activity
C0030424cellular_componentaxon
C0030863cellular_componentcortical cytoskeleton
C0030957molecular_functionTat protein binding
C0032991cellular_componentprotein-containing complex
C0034333biological_processadherens junction assembly
C0035267cellular_componentNuA4 histone acetyltransferase complex
C0036464cellular_componentcytoplasmic ribonucleoprotein granule
C0042802molecular_functionidentical protein binding
C0043296cellular_componentapical junction complex
C0044305cellular_componentcalyx of Held
C0045176biological_processapical protein localization
C0045202cellular_componentsynapse
C0048870biological_processcell motility
C0050998molecular_functionnitric-oxide synthase binding
C0051621biological_processregulation of norepinephrine uptake
C0051726biological_processregulation of cell cycle
C0070160cellular_componenttight junction
C0071896biological_processprotein localization to adherens junction
C0072749biological_processcellular response to cytochalasin B
C0097433cellular_componentdense body
C0098685cellular_componentSchaffer collateral - CA1 synapse
C0098871cellular_componentpostsynaptic actin cytoskeleton
C0098973molecular_functionstructural constituent of postsynaptic actin cytoskeleton
C0098974biological_processpostsynaptic actin cytoskeleton organization
C0098978cellular_componentglutamatergic synapse
C0141108molecular_functiontransporter regulator activity
C0150111biological_processregulation of transepithelial transport
C1900242biological_processregulation of synaptic vesicle endocytosis
C1903076biological_processregulation of protein localization to plasma membrane
C1905168biological_processpositive regulation of double-strand break repair via homologous recombination
C1990904cellular_componentribonucleoprotein complex
D0000166molecular_functionnucleotide binding
D0000786cellular_componentnucleosome
D0001738biological_processmorphogenesis of a polarized epithelium
D0005515molecular_functionprotein binding
D0005524molecular_functionATP binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005884cellular_componentactin filament
D0005886cellular_componentplasma membrane
D0005903cellular_componentbrush border
D0005911cellular_componentcell-cell junction
D0005912cellular_componentadherens junction
D0005925cellular_componentfocal adhesion
D0007163biological_processestablishment or maintenance of cell polarity
D0007409biological_processaxonogenesis
D0015629cellular_componentactin cytoskeleton
D0016020cellular_componentmembrane
D0016787molecular_functionhydrolase activity
D0016887molecular_functionATP hydrolysis activity
D0019894molecular_functionkinesin binding
D0019901molecular_functionprotein kinase binding
D0030027cellular_componentlamellipodium
D0030235molecular_functionnitric-oxide synthase regulator activity
D0030424cellular_componentaxon
D0030863cellular_componentcortical cytoskeleton
D0030957molecular_functionTat protein binding
D0032991cellular_componentprotein-containing complex
D0034333biological_processadherens junction assembly
D0035267cellular_componentNuA4 histone acetyltransferase complex
D0036464cellular_componentcytoplasmic ribonucleoprotein granule
D0042802molecular_functionidentical protein binding
D0043296cellular_componentapical junction complex
D0044305cellular_componentcalyx of Held
D0045176biological_processapical protein localization
D0045202cellular_componentsynapse
D0048870biological_processcell motility
D0050998molecular_functionnitric-oxide synthase binding
D0051621biological_processregulation of norepinephrine uptake
D0051726biological_processregulation of cell cycle
D0070160cellular_componenttight junction
D0071896biological_processprotein localization to adherens junction
D0072749biological_processcellular response to cytochalasin B
D0097433cellular_componentdense body
D0098685cellular_componentSchaffer collateral - CA1 synapse
D0098871cellular_componentpostsynaptic actin cytoskeleton
D0098973molecular_functionstructural constituent of postsynaptic actin cytoskeleton
D0098974biological_processpostsynaptic actin cytoskeleton organization
D0098978cellular_componentglutamatergic synapse
D0141108molecular_functiontransporter regulator activity
D0150111biological_processregulation of transepithelial transport
D1900242biological_processregulation of synaptic vesicle endocytosis
D1903076biological_processregulation of protein localization to plasma membrane
D1905168biological_processpositive regulation of double-strand break repair via homologous recombination
D1990904cellular_componentribonucleoprotein complex
E0003824molecular_functioncatalytic activity
E0004536molecular_functionDNA nuclease activity
E0006308biological_processDNA catabolic process
F0003824molecular_functioncatalytic activity
F0004536molecular_functionDNA nuclease activity
F0006308biological_processDNA catabolic process
Functional Information from PROSITE/UniProt
site_idPS00406
Number of Residues11
DetailsACTINS_1 Actins signature 1. YVGDEAQs.KRG
ChainResidueDetails
ATYR53-GLY63

site_idPS00432
Number of Residues9
DetailsACTINS_2 Actins signature 2. WISKqEYDE
ChainResidueDetails
ATRP356-GLU364

site_idPS00918
Number of Residues8
DetailsDNASE_I_2 Deoxyribonuclease I signature 2. GDFNAdCS
ChainResidueDetails
EGLY167-SER174

site_idPS00919
Number of Residues21
DetailsDNASE_I_1 Deoxyribonuclease I signature 1. IVALHSAPsdavaEINsLyDV
ChainResidueDetails
EILE130-VAL150

site_idPS01132
Number of Residues13
DetailsACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR
ChainResidueDetails
ALEU104-ARG116

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2
DetailsACT_SITE: ACT_SITE => ECO:0000269|PubMed:2395459
ChainResidueDetails
EGLU78
FGLU78
CASP2
DASP2

site_idSWS_FT_FI2
Number of Residues2
DetailsACT_SITE: ACT_SITE => ECO:0000269|PubMed:2395459, ECO:0000269|PubMed:4976790
ChainResidueDetails
EHIS134
FHIS134
BMET44
BMET47
CMET44
CMET47
DMET44
DMET47

site_idSWS_FT_FI3
Number of Residues4
DetailsSITE: Involved in actin-binding => ECO:0000269|PubMed:2395459
ChainResidueDetails
EGLU13
EVAL67
FGLU13
FVAL67

site_idSWS_FT_FI4
Number of Residues2
DetailsSITE: Nitration by tetranitromethane destroys a Ca(2+) binding site and inactivates enzyme
ChainResidueDetails
ETYR65
FTYR65
CLYS84
DLYS84

site_idSWS_FT_FI5
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:1748997, ECO:0000269|PubMed:3560229
ChainResidueDetails
EASN18
FASN18

Catalytic Information from CSA
site_idMCSA1
Number of Residues7
DetailsM-CSA 41
ChainResidueDetails
EGLU39metal ligand
ETYR76electrostatic stabiliser
EGLU78electrostatic stabiliser, increase acidity, increase basicity
EHIS134proton acceptor, proton donor
EASP168metal ligand
EASP212electrostatic stabiliser, increase acidity, increase basicity
EHIS252proton acceptor, proton donor

site_idMCSA2
Number of Residues7
DetailsM-CSA 41
ChainResidueDetails
FGLU39metal ligand
FTYR76electrostatic stabiliser
FGLU78electrostatic stabiliser, increase acidity, increase basicity
FHIS134proton acceptor, proton donor
FASP168metal ligand
FASP212electrostatic stabiliser, increase acidity, increase basicity
FHIS252proton acceptor, proton donor

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PDB entries from 2025-07-02

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