9EIJ

Import stalled PINK1 TOM complex, extended TOM20 helix class

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0000422	biological_process	autophagy of mitochondrion
B	0000423	biological_process	mitophagy
B	0000785	cellular_component	chromatin
B	0002020	molecular_function	protease binding
B	0002082	biological_process	regulation of oxidative phosphorylation
B	0002931	biological_process	response to ischemia
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005741	cellular_component	mitochondrial outer membrane
B	0005743	cellular_component	mitochondrial inner membrane
B	0005758	cellular_component	mitochondrial intermembrane space
B	0005783	cellular_component	endoplasmic reticulum
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0006468	biological_process	protein phosphorylation
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0006914	biological_process	autophagy
B	0006950	biological_process	response to stress
B	0006979	biological_process	response to oxidative stress
B	0007005	biological_process	mitochondrion organization
B	0010310	biological_process	regulation of hydrogen peroxide metabolic process
B	0010629	biological_process	negative regulation of gene expression
B	0010821	biological_process	regulation of mitochondrion organization
B	0016020	cellular_component	membrane
B	0016236	biological_process	macroautophagy
B	0016239	biological_process	positive regulation of macroautophagy
B	0016242	biological_process	negative regulation of macroautophagy
B	0016301	molecular_function	kinase activity
B	0016504	molecular_function	peptidase activator activity
B	0016567	biological_process	protein ubiquitination
B	0016740	molecular_function	transferase activity
B	0019900	molecular_function	kinase binding
B	0022904	biological_process	respiratory electron transport chain
B	0030097	biological_process	hemopoiesis
B	0030335	biological_process	positive regulation of cell migration
B	0030424	cellular_component	axon
B	0030426	cellular_component	growth cone
B	0031396	biological_process	regulation of protein ubiquitination
B	0031398	biological_process	positive regulation of protein ubiquitination
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0034599	biological_process	cellular response to oxidative stress
B	0035556	biological_process	intracellular signal transduction
B	0038203	biological_process	TORC2 signaling
B	0042981	biological_process	regulation of apoptotic process
B	0043066	biological_process	negative regulation of apoptotic process
B	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
B	0043254	biological_process	regulation of protein-containing complex assembly
B	0043422	molecular_function	protein kinase B binding
B	0043523	biological_process	regulation of neuron apoptotic process
B	0043524	biological_process	negative regulation of neuron apoptotic process
B	0044297	cellular_component	cell body
B	0044877	molecular_function	protein-containing complex binding
B	0045727	biological_process	positive regulation of translation
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0046329	biological_process	negative regulation of JNK cascade
B	0046872	molecular_function	metal ion binding
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050821	biological_process	protein stabilization
B	0051881	biological_process	regulation of mitochondrial membrane potential
B	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
B	0055131	molecular_function	C3HC4-type RING finger domain binding
B	0061136	biological_process	regulation of proteasomal protein catabolic process
B	0071456	biological_process	cellular response to hypoxia
B	0072655	biological_process	establishment of protein localization to mitochondrion
B	0072656	biological_process	maintenance of protein location in mitochondrion
B	0090141	biological_process	positive regulation of mitochondrial fission
B	0090200	biological_process	positive regulation of release of cytochrome c from mitochondria
B	0090258	biological_process	negative regulation of mitochondrial fission
B	0097237	biological_process	cellular response to toxic substance
B	0097413	cellular_component	Lewy body
B	0097449	cellular_component	astrocyte projection
B	0099074	biological_process	mitochondrion to lysosome vesicle-mediated transport
B	0106310	molecular_function	protein serine kinase activity
B	1900407	biological_process	regulation of cellular response to oxidative stress
B	1901525	biological_process	negative regulation of mitophagy
B	1902803	biological_process	regulation of synaptic vesicle transport
B	1902902	biological_process	negative regulation of autophagosome assembly
B	1902958	biological_process	positive regulation of mitochondrial electron transport, NADH to ubiquinone
B	1903146	biological_process	regulation of autophagy of mitochondrion
B	1903214	biological_process	regulation of protein targeting to mitochondrion
B	1903298	biological_process	negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
B	1903377	biological_process	negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
B	1903384	biological_process	negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway
B	1903751	biological_process	negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide
B	1903852	biological_process	positive regulation of cristae formation
B	1903955	biological_process	positive regulation of protein targeting to mitochondrion
B	1904544	biological_process	positive regulation of free ubiquitin chain polymerization
B	1904881	biological_process	cellular response to hydrogen sulfide
B	1905091	biological_process	positive regulation of type 2 mitophagy
B	2000377	biological_process	regulation of reactive oxygen species metabolic process
B	2000378	biological_process	negative regulation of reactive oxygen species metabolic process
B	2001171	biological_process	positive regulation of ATP biosynthetic process
B	2001243	biological_process	negative regulation of intrinsic apoptotic signaling pathway
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0005740	cellular_component	mitochondrial envelope
D	0005741	cellular_component	mitochondrial outer membrane
D	0005742	cellular_component	mitochondrial outer membrane translocase complex
D	0006605	biological_process	protein targeting
D	0006626	biological_process	protein targeting to mitochondrion
D	0006886	biological_process	intracellular protein transport
D	0008320	molecular_function	protein transmembrane transporter activity
D	0015031	biological_process	protein transport
D	0015450	molecular_function	protein-transporting ATPase activity
D	0016031	biological_process	tRNA import into mitochondrion
D	0030150	biological_process	protein import into mitochondrial matrix
D	0030943	molecular_function	mitochondrion targeting sequence binding
D	0044233	cellular_component	mitochondria-associated endoplasmic reticulum membrane contact site
D	0045040	biological_process	protein insertion into mitochondrial outer membrane
D	0051082	molecular_function	unfolded protein binding
D	0071944	cellular_component	cell periphery
D	0097225	cellular_component	sperm midpiece
D	0140494	cellular_component	migrasome
E	0000166	molecular_function	nucleotide binding
E	0001669	cellular_component	acrosomal vesicle
E	0005244	molecular_function	voltage-gated monoatomic ion channel activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005739	cellular_component	mitochondrion
E	0005741	cellular_component	mitochondrial outer membrane
E	0006811	biological_process	monoatomic ion transport
E	0006820	biological_process	monoatomic anion transport
E	0006869	biological_process	lipid transport
E	0007339	biological_process	binding of sperm to zona pellucida
E	0008142	molecular_function	oxysterol binding
E	0008289	molecular_function	lipid binding
E	0008308	molecular_function	voltage-gated monoatomic anion channel activity
E	0015288	molecular_function	porin activity
E	0015485	molecular_function	cholesterol binding
E	0016020	cellular_component	membrane
E	0017121	biological_process	plasma membrane phospholipid scrambling
E	0017128	molecular_function	phospholipid scramblase activity
E	0031210	molecular_function	phosphatidylcholine binding
E	0031966	cellular_component	mitochondrial membrane
E	0042645	cellular_component	mitochondrial nucleoid
E	0045332	biological_process	phospholipid translocation
E	0046930	cellular_component	pore complex
E	0055085	biological_process	transmembrane transport
E	0097001	molecular_function	ceramide binding
E	0097225	cellular_component	sperm midpiece
E	0097345	biological_process	mitochondrial outer membrane permeabilization
E	0098656	biological_process	monoatomic anion transmembrane transport
E	1990542	biological_process	mitochondrial transmembrane transport
F	0000166	molecular_function	nucleotide binding
F	0001669	cellular_component	acrosomal vesicle
F	0005244	molecular_function	voltage-gated monoatomic ion channel activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005739	cellular_component	mitochondrion
F	0005741	cellular_component	mitochondrial outer membrane
F	0006811	biological_process	monoatomic ion transport
F	0006820	biological_process	monoatomic anion transport
F	0006869	biological_process	lipid transport
F	0007339	biological_process	binding of sperm to zona pellucida
F	0008142	molecular_function	oxysterol binding
F	0008289	molecular_function	lipid binding
F	0008308	molecular_function	voltage-gated monoatomic anion channel activity
F	0015288	molecular_function	porin activity
F	0015485	molecular_function	cholesterol binding
F	0016020	cellular_component	membrane
F	0017121	biological_process	plasma membrane phospholipid scrambling
F	0017128	molecular_function	phospholipid scramblase activity
F	0031210	molecular_function	phosphatidylcholine binding
F	0031966	cellular_component	mitochondrial membrane
F	0042645	cellular_component	mitochondrial nucleoid
F	0045332	biological_process	phospholipid translocation
F	0046930	cellular_component	pore complex
F	0055085	biological_process	transmembrane transport
F	0097001	molecular_function	ceramide binding
F	0097225	cellular_component	sperm midpiece
F	0097345	biological_process	mitochondrial outer membrane permeabilization
F	0098656	biological_process	monoatomic anion transmembrane transport
F	1990542	biological_process	mitochondrial transmembrane transport
I	0005515	molecular_function	protein binding
I	0005739	cellular_component	mitochondrion
I	0005741	cellular_component	mitochondrial outer membrane
I	0005742	cellular_component	mitochondrial outer membrane translocase complex
I	0005743	cellular_component	mitochondrial inner membrane
I	0006626	biological_process	protein targeting to mitochondrion
I	0006811	biological_process	monoatomic ion transport
I	0008320	molecular_function	protein transmembrane transporter activity
I	0015031	biological_process	protein transport
I	0015288	molecular_function	porin activity
I	0016020	cellular_component	membrane
I	0030150	biological_process	protein import into mitochondrial matrix
I	0044233	cellular_component	mitochondria-associated endoplasmic reticulum membrane contact site
I	0045040	biological_process	protein insertion into mitochondrial outer membrane
I	0046930	cellular_component	pore complex
I	0055085	biological_process	transmembrane transport
J	0005515	molecular_function	protein binding
J	0005739	cellular_component	mitochondrion
J	0005741	cellular_component	mitochondrial outer membrane
J	0005742	cellular_component	mitochondrial outer membrane translocase complex
J	0005743	cellular_component	mitochondrial inner membrane
J	0006626	biological_process	protein targeting to mitochondrion
J	0006811	biological_process	monoatomic ion transport
J	0008320	molecular_function	protein transmembrane transporter activity
J	0015031	biological_process	protein transport
J	0015288	molecular_function	porin activity
J	0016020	cellular_component	membrane
J	0030150	biological_process	protein import into mitochondrial matrix
J	0044233	cellular_component	mitochondria-associated endoplasmic reticulum membrane contact site
J	0045040	biological_process	protein insertion into mitochondrial outer membrane
J	0046930	cellular_component	pore complex
J	0055085	biological_process	transmembrane transport
K	0005739	cellular_component	mitochondrion
K	0005741	cellular_component	mitochondrial outer membrane
K	0005742	cellular_component	mitochondrial outer membrane translocase complex
K	0006626	biological_process	protein targeting to mitochondrion
K	0015031	biological_process	protein transport
K	0045040	biological_process	protein insertion into mitochondrial outer membrane
L	0005739	cellular_component	mitochondrion
L	0005741	cellular_component	mitochondrial outer membrane
L	0005742	cellular_component	mitochondrial outer membrane translocase complex
L	0006626	biological_process	protein targeting to mitochondrion
L	0015031	biological_process	protein transport
L	0045040	biological_process	protein insertion into mitochondrial outer membrane
N	0005515	molecular_function	protein binding
N	0005739	cellular_component	mitochondrion
N	0005741	cellular_component	mitochondrial outer membrane
N	0005742	cellular_component	mitochondrial outer membrane translocase complex
N	0008320	molecular_function	protein transmembrane transporter activity
N	0015031	biological_process	protein transport
N	0030150	biological_process	protein import into mitochondrial matrix
N	0031647	biological_process	regulation of protein stability
N	0045040	biological_process	protein insertion into mitochondrial outer membrane
N	1903955	biological_process	positive regulation of protein targeting to mitochondrion
N	1905091	biological_process	positive regulation of type 2 mitophagy
P	0005515	molecular_function	protein binding
P	0005739	cellular_component	mitochondrion
P	0005741	cellular_component	mitochondrial outer membrane
P	0005742	cellular_component	mitochondrial outer membrane translocase complex
P	0015031	biological_process	protein transport
P	0045040	biological_process	protein insertion into mitochondrial outer membrane
R	0005515	molecular_function	protein binding
R	0005739	cellular_component	mitochondrion
R	0005741	cellular_component	mitochondrial outer membrane
R	0005742	cellular_component	mitochondrial outer membrane translocase complex
R	0006626	biological_process	protein targeting to mitochondrion
R	0006886	biological_process	intracellular protein transport
R	0008320	molecular_function	protein transmembrane transporter activity
R	0015031	biological_process	protein transport
R	0016020	cellular_component	membrane
R	0030150	biological_process	protein import into mitochondrial matrix
R	0030943	molecular_function	mitochondrion targeting sequence binding
R	0045040	biological_process	protein insertion into mitochondrial outer membrane
T	0005515	molecular_function	protein binding
T	0005739	cellular_component	mitochondrion
T	0005741	cellular_component	mitochondrial outer membrane
T	0005742	cellular_component	mitochondrial outer membrane translocase complex
T	0006626	biological_process	protein targeting to mitochondrion
T	0006886	biological_process	intracellular protein transport
T	0008320	molecular_function	protein transmembrane transporter activity
T	0015031	biological_process	protein transport
T	0016020	cellular_component	membrane
T	0030150	biological_process	protein import into mitochondrial matrix
T	0030943	molecular_function	mitochondrion targeting sequence binding
T	0045040	biological_process	protein insertion into mitochondrial outer membrane
V	0005515	molecular_function	protein binding
V	0005739	cellular_component	mitochondrion
V	0005741	cellular_component	mitochondrial outer membrane
V	0005742	cellular_component	mitochondrial outer membrane translocase complex
V	0015031	biological_process	protein transport
V	0045040	biological_process	protein insertion into mitochondrial outer membrane
X	0005515	molecular_function	protein binding
X	0005739	cellular_component	mitochondrion
X	0005741	cellular_component	mitochondrial outer membrane
X	0005742	cellular_component	mitochondrial outer membrane translocase complex
X	0008320	molecular_function	protein transmembrane transporter activity
X	0015031	biological_process	protein transport
X	0030150	biological_process	protein import into mitochondrial matrix
X	0031647	biological_process	regulation of protein stability
X	0045040	biological_process	protein insertion into mitochondrial outer membrane
X	1903955	biological_process	positive regulation of protein targeting to mitochondrion
X	1905091	biological_process	positive regulation of type 2 mitophagy
Z	0005739	cellular_component	mitochondrion
Z	0005741	cellular_component	mitochondrial outer membrane
Z	0005742	cellular_component	mitochondrial outer membrane translocase complex
Z	0006626	biological_process	protein targeting to mitochondrion
Z	0015031	biological_process	protein transport
Z	0045040	biological_process	protein insertion into mitochondrial outer membrane

Functional Information from PROSITE/UniProt

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IaHrDLKsdNILV

Chain	Residue	Details
B	ILE358-VAL370

---

site_id	PS00558
Number of Residues	23
Details	EUKARYOTIC_PORIN Eukaryotic mitochondrial porin signature. YqlDPtAsisAKVNNssliGVgY

Chain	Residue	Details
E	TYR236-TYR258

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	Topological domain: {"description":"Mitochondrial intermembrane","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	155
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	13
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"25621951","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"24896179","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"24896179","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25621951","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	328
Details	Transmembrane: {"description":"Beta stranded","evidences":[{"source":"UniProtKB","id":"P21796","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q60932","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P21796","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Site: {"description":"Involved in ceramide and phosphatidylcholine binding","evidences":[{"source":"PubMed","id":"31015432","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"Q60930","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"Q60932","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P21796","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"Q60930","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P21796","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P21796","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	6
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"PubMed","id":"25621951","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P21796","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	1
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"25944712","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	19
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"12198123","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	36
Details	Topological domain: {"description":"Mitochondrial intermembrane","evidences":[{"source":"PubMed","id":"12198123","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	40
Details	Region: {"description":"TMD; necessary for mitochondrion outer membrane localization and integration in the TOM complex","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	6
Details	Region: {"description":"Required for outer membrane localization","evidences":[{"source":"PubMed","id":"30733118","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q02750","evidenceCode":"ECO:0000250"},{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by autocatalysis","evidences":[{"source":"PubMed","id":"22910362","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---