9E3C

Cryo-EM structure of PRMT5/WDR77 in complex with 6S complex (GRG local refine)

Functional Information from GO Data

Chain	GOid	namespace	contents
J	0000387	biological_process	spliceosomal snRNP assembly
J	0000785	cellular_component	chromatin
J	0002039	molecular_function	p53 binding
J	0003714	molecular_function	transcription corepressor activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005694	cellular_component	chromosome
J	0005737	cellular_component	cytoplasm
J	0005794	cellular_component	Golgi apparatus
J	0005829	cellular_component	cytosol
J	0006325	biological_process	chromatin organization
J	0006338	biological_process	chromatin remodeling
J	0006353	biological_process	DNA-templated transcription termination
J	0006355	biological_process	regulation of DNA-templated transcription
J	0007088	biological_process	regulation of mitotic nuclear division
J	0008168	molecular_function	methyltransferase activity
J	0008327	molecular_function	methyl-CpG binding
J	0008469	molecular_function	histone arginine N-methyltransferase activity
J	0010468	biological_process	regulation of gene expression
J	0016274	molecular_function	protein-arginine N-methyltransferase activity
J	0016740	molecular_function	transferase activity
J	0018216	biological_process	peptidyl-arginine methylation
J	0032259	biological_process	methylation
J	0032922	biological_process	circadian regulation of gene expression
J	0032991	cellular_component	protein-containing complex
J	0034709	cellular_component	methylosome
J	0035097	cellular_component	histone methyltransferase complex
J	0035243	molecular_function	protein-arginine omega-N symmetric methyltransferase activity
J	0035246	biological_process	peptidyl-arginine N-methylation
J	0042054	molecular_function	histone methyltransferase activity
J	0042118	biological_process	endothelial cell activation
J	0042802	molecular_function	identical protein binding
J	0043021	molecular_function	ribonucleoprotein complex binding
J	0044020	molecular_function	histone H4R3 methyltransferase activity
J	0044027	biological_process	negative regulation of gene expression via chromosomal CpG island methylation
J	0044877	molecular_function	protein-containing complex binding
J	0045596	biological_process	negative regulation of cell differentiation
J	0045892	biological_process	negative regulation of DNA-templated transcription
J	0046982	molecular_function	protein heterodimerization activity
J	0048026	biological_process	positive regulation of mRNA splicing, via spliceosome
J	0048511	biological_process	rhythmic process
J	0048714	biological_process	positive regulation of oligodendrocyte differentiation
J	0070372	biological_process	regulation of ERK1 and ERK2 cascade
J	0070888	molecular_function	E-box binding
J	0090161	biological_process	Golgi ribbon formation
J	0097421	biological_process	liver regeneration
J	0140938	molecular_function	histone H3 methyltransferase activity
J	1901796	biological_process	regulation of signal transduction by p53 class mediator
J	1904992	biological_process	positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway
M	0000243	cellular_component	commitment complex
M	0000245	biological_process	spliceosomal complex assembly
M	0000387	biological_process	spliceosomal snRNP assembly
M	0000398	biological_process	mRNA splicing, via spliceosome
M	0003723	molecular_function	RNA binding
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005681	cellular_component	spliceosomal complex
M	0005682	cellular_component	U5 snRNP
M	0005684	cellular_component	U2-type spliceosomal complex
M	0005685	cellular_component	U1 snRNP
M	0005686	cellular_component	U2 snRNP
M	0005687	cellular_component	U4 snRNP
M	0005689	cellular_component	U12-type spliceosomal complex
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0006397	biological_process	mRNA processing
M	0008380	biological_process	RNA splicing
M	0030532	cellular_component	small nuclear ribonucleoprotein complex
M	0034709	cellular_component	methylosome
M	0034715	cellular_component	pICln-Sm protein complex
M	0034719	cellular_component	SMN-Sm protein complex
M	0036261	biological_process	7-methylguanosine cap hypermethylation
M	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
M	0071005	cellular_component	U2-type precatalytic spliceosome
M	0071007	cellular_component	U2-type catalytic step 2 spliceosome
M	0071011	cellular_component	precatalytic spliceosome
M	0071013	cellular_component	catalytic step 2 spliceosome
M	0097526	cellular_component	spliceosomal tri-snRNP complex
M	1903241	biological_process	U2-type prespliceosome assembly
M	1990446	molecular_function	U1 snRNP binding
M	1990904	cellular_component	ribonucleoprotein complex

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
M	LYS86
J	GLU444

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site_id	SWS_FT_FI2
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269|PubMed:23071334

Chain	Residue	Details
J	TYR324
J	PHE327
J	LYS333
J	GLU392
J	ASP419
J	GLU435
J	GLU444

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site_id	SWS_FT_FI3
Number of Residues	1
Details	SITE: Critical for specifying symmetric addition of methyl groups => ECO:0000250|UniProtKB:P46580

Chain	Residue	Details
J	PHE327

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N-acetylalanine; in Protein arginine N-methyltransferase 5, N-terminally processed => ECO:0000269|Ref.15, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378

Chain	Residue	Details
J	ALA2

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