9DW4
Dephosphorylated CFTR in 1:1 complex with PKA-C (site II)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0005254 | molecular_function | chloride channel activity |
| A | 0005260 | molecular_function | intracellularly ATP-gated chloride channel activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005765 | cellular_component | lysosomal membrane |
| A | 0005768 | cellular_component | endosome |
| A | 0005769 | cellular_component | early endosome |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006695 | biological_process | cholesterol biosynthetic process |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0006821 | biological_process | chloride transport |
| A | 0006833 | biological_process | water transport |
| A | 0006904 | biological_process | vesicle docking involved in exocytosis |
| A | 0009986 | cellular_component | cell surface |
| A | 0010008 | cellular_component | endosome membrane |
| A | 0015106 | molecular_function | bicarbonate transmembrane transporter activity |
| A | 0015108 | molecular_function | chloride transmembrane transporter activity |
| A | 0015701 | biological_process | bicarbonate transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016323 | cellular_component | basolateral plasma membrane |
| A | 0016324 | cellular_component | apical plasma membrane |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0016887 | molecular_function | ATP hydrolysis activity |
| A | 0017081 | molecular_function | chloride channel regulator activity |
| A | 0019869 | molecular_function | chloride channel inhibitor activity |
| A | 0019899 | molecular_function | enzyme binding |
| A | 0030165 | molecular_function | PDZ domain binding |
| A | 0030301 | biological_process | cholesterol transport |
| A | 0030660 | cellular_component | Golgi-associated vesicle membrane |
| A | 0030669 | cellular_component | clathrin-coated endocytic vesicle membrane |
| A | 0031901 | cellular_component | early endosome membrane |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0034220 | biological_process | monoatomic ion transmembrane transport |
| A | 0034707 | cellular_component | chloride channel complex |
| A | 0034976 | biological_process | response to endoplasmic reticulum stress |
| A | 0035377 | biological_process | transepithelial water transport |
| A | 0035774 | biological_process | positive regulation of insulin secretion involved in cellular response to glucose stimulus |
| A | 0043225 | molecular_function | ATPase-coupled inorganic anion transmembrane transporter activity |
| A | 0045921 | biological_process | positive regulation of exocytosis |
| A | 0048240 | biological_process | sperm capacitation |
| A | 0050891 | biological_process | multicellular organismal-level water homeostasis |
| A | 0051087 | molecular_function | protein-folding chaperone binding |
| A | 0051454 | biological_process | intracellular pH elevation |
| A | 0051649 | biological_process | establishment of localization in cell |
| A | 0055037 | cellular_component | recycling endosome |
| A | 0055038 | cellular_component | recycling endosome membrane |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0060081 | biological_process | membrane hyperpolarization |
| A | 0065008 | biological_process | regulation of biological quality |
| A | 0070175 | biological_process | positive regulation of enamel mineralization |
| A | 0071320 | biological_process | cellular response to cAMP |
| A | 0071889 | molecular_function | 14-3-3 protein binding |
| A | 0097186 | biological_process | amelogenesis |
| A | 0098660 | biological_process | inorganic ion transmembrane transport |
| A | 0106138 | molecular_function | Sec61 translocon complex binding |
| A | 0140359 | molecular_function | ABC-type transporter activity |
| A | 1902476 | biological_process | chloride transmembrane transport |
| A | 1902943 | biological_process | positive regulation of voltage-gated chloride channel activity |
| A | 1904322 | biological_process | cellular response to forskolin |
| K | 0000166 | molecular_function | nucleotide binding |
| K | 0000287 | molecular_function | magnesium ion binding |
| K | 0001669 | cellular_component | acrosomal vesicle |
| K | 0001707 | biological_process | mesoderm formation |
| K | 0001843 | biological_process | neural tube closure |
| K | 0004672 | molecular_function | protein kinase activity |
| K | 0004674 | molecular_function | protein serine/threonine kinase activity |
| K | 0004691 | molecular_function | cAMP-dependent protein kinase activity |
| K | 0004712 | molecular_function | protein serine/threonine/tyrosine kinase activity |
| K | 0005515 | molecular_function | protein binding |
| K | 0005524 | molecular_function | ATP binding |
| K | 0005634 | cellular_component | nucleus |
| K | 0005737 | cellular_component | cytoplasm |
| K | 0005739 | cellular_component | mitochondrion |
| K | 0005813 | cellular_component | centrosome |
| K | 0005829 | cellular_component | cytosol |
| K | 0005886 | cellular_component | plasma membrane |
| K | 0005930 | cellular_component | axoneme |
| K | 0005952 | cellular_component | cAMP-dependent protein kinase complex |
| K | 0006397 | biological_process | mRNA processing |
| K | 0006468 | biological_process | protein phosphorylation |
| K | 0006611 | biological_process | protein export from nucleus |
| K | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| K | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| K | 0016020 | cellular_component | membrane |
| K | 0016301 | molecular_function | kinase activity |
| K | 0016607 | cellular_component | nuclear speck |
| K | 0016740 | molecular_function | transferase activity |
| K | 0018105 | biological_process | peptidyl-serine phosphorylation |
| K | 0019901 | molecular_function | protein kinase binding |
| K | 0019904 | molecular_function | protein domain specific binding |
| K | 0030007 | biological_process | intracellular potassium ion homeostasis |
| K | 0030145 | molecular_function | manganese ion binding |
| K | 0031594 | cellular_component | neuromuscular junction |
| K | 0031625 | molecular_function | ubiquitin protein ligase binding |
| K | 0032024 | biological_process | positive regulation of insulin secretion |
| K | 0034237 | molecular_function | protein kinase A regulatory subunit binding |
| K | 0034605 | biological_process | cellular response to heat |
| K | 0036126 | cellular_component | sperm flagellum |
| K | 0044853 | cellular_component | plasma membrane raft |
| K | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| K | 0045667 | biological_process | regulation of osteoblast differentiation |
| K | 0045722 | biological_process | positive regulation of gluconeogenesis |
| K | 0045879 | biological_process | negative regulation of smoothened signaling pathway |
| K | 0046827 | biological_process | positive regulation of protein export from nucleus |
| K | 0048240 | biological_process | sperm capacitation |
| K | 0048471 | cellular_component | perinuclear region of cytoplasm |
| K | 0050766 | biological_process | positive regulation of phagocytosis |
| K | 0050804 | biological_process | modulation of chemical synaptic transmission |
| K | 0051726 | biological_process | regulation of cell cycle |
| K | 0061136 | biological_process | regulation of proteasomal protein catabolic process |
| K | 0070417 | biological_process | cellular response to cold |
| K | 0070613 | biological_process | regulation of protein processing |
| K | 0071333 | biological_process | cellular response to glucose stimulus |
| K | 0071374 | biological_process | cellular response to parathyroid hormone stimulus |
| K | 0071377 | biological_process | cellular response to glucagon stimulus |
| K | 0097546 | cellular_component | ciliary base |
| K | 0098794 | cellular_component | postsynapse |
| K | 0098978 | cellular_component | glutamatergic synapse |
| K | 0099170 | biological_process | postsynaptic modulation of chemical synaptic transmission |
| K | 0106310 | molecular_function | protein serine kinase activity |
| K | 1904262 | biological_process | negative regulation of TORC1 signaling |
| K | 1904539 | biological_process | negative regulation of glycolytic process through fructose-6-phosphate |
| K | 1990044 | biological_process | protein localization to lipid droplet |
| K | 2000810 | biological_process | regulation of bicellular tight junction assembly |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 24 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGTGSFGRVMlVkhmetgnh..........YAMK |
| Chain | Residue | Details |
| K | LEU50-LYS73 |
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. LiYrDLKpeNLLI |
| Chain | Residue | Details |
| K | LEU163-ILE175 |
| site_id | PS00211 |
| Number of Residues | 15 |
| Details | ABC_TRANSPORTER_1 ABC transporters family signature. LSGGQRARISLARAV |
| Chain | Residue | Details |
| A | LEU548-VAL562 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 76 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000305 |
| Chain | Residue | Details |
| A | MET1-PHE77 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=1 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | PHE78-GLN98 | |
| K | LYS73 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 99 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | PRO99-TYR122 | |
| A | GLU217-SER222 | |
| A | LEU320-THR339 | |
| A | VAL880-ILE918 | |
| A | GLN1012-PRO1013 | |
| A | ILE1117-GLY1130 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=2 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | LEU123-HIS146 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1039 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | HIS147-LEU195 | |
| A | MET244-LYS298 | |
| A | GLN359-SER858 | |
| A | THR940-THR990 | |
| A | GLN1035-THR1095 | |
| A | ASP1152-LEU1480 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | ALA196-TRP216 | |
| K | THR196 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | ALA223-MET243 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | ALA299-PHE319 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 18 |
| Details | TRANSMEM: Helical; Name=6 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | ILE340-VAL358 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | LEU859-VAL879 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 20 |
| Details | TRANSMEM: Discontinuously helical; Name=8 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | TYR919-HIS939 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=9 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | ILE991-LEU1011 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=10 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | TYR1014-LEU1034 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=11 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | LEU1096-PHE1116 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=12 => ECO:0000269|Ref.56 |
| Chain | Residue | Details |
| A | ILE1131-ILE1151 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15528182, ECO:0000269|PubMed:20150177, ECO:0007744|PDB:1XMI, ECO:0007744|PDB:1XMJ, ECO:0007744|PDB:2BBO, ECO:0007744|PDB:2BBS, ECO:0007744|PDB:2BBT, ECO:0007744|PDB:2PZE, ECO:0007744|PDB:2PZF, ECO:0007744|PDB:2PZG |
| Chain | Residue | Details |
| A | TRP401 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:20150177, ECO:0007744|PDB:2PZE, ECO:0007744|PDB:2PZF, ECO:0007744|PDB:2PZG |
| Chain | Residue | Details |
| A | SER434 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00434, ECO:0000269|PubMed:15528182, ECO:0007744|PDB:1XMI, ECO:0007744|PDB:1XMJ, ECO:0007744|PDB:2BBO, ECO:0007744|PDB:2BBS, ECO:0007744|PDB:2BBT |
| Chain | Residue | Details |
| A | GLY458 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15528182, ECO:0007744|PDB:1XMI, ECO:0007744|PDB:2BBO, ECO:0007744|PDB:2BBS |
| Chain | Residue | Details |
| A | GLN493 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0007744|PDB:3GD7 |
| Chain | Residue | Details |
| A | TYR1219 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00434, ECO:0007744|PDB:3GD7 |
| Chain | Residue | Details |
| A | GLY1244 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231 |
| Chain | Residue | Details |
| A | SER549 |
| site_id | SWS_FT_FI23 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:1377674, ECO:0000269|PubMed:22119790, ECO:0000269|PubMed:25330774, ECO:0000269|PubMed:9385646 |
| Chain | Residue | Details |
| A | SER660 | |
| A | SER700 | |
| A | SER737 | |
| A | SER795 |
| site_id | SWS_FT_FI24 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:25330774 |
| Chain | Residue | Details |
| A | SER670 |
| site_id | SWS_FT_FI25 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PKC => ECO:0000269|PubMed:1377674, ECO:0000269|PubMed:22119790 |
| Chain | Residue | Details |
| A | SER686 |
| site_id | SWS_FT_FI26 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:22119790, ECO:0000269|PubMed:25330774, ECO:0000269|PubMed:9385646 |
| Chain | Residue | Details |
| A | SER712 |
| site_id | SWS_FT_FI27 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0000269|PubMed:22119790 |
| Chain | Residue | Details |
| A | THR717 |
| site_id | SWS_FT_FI28 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:25330774, ECO:0000269|PubMed:9385646 |
| Chain | Residue | Details |
| A | SER753 |
| site_id | SWS_FT_FI29 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:1377674, ECO:0000269|PubMed:25330774, ECO:0000269|PubMed:9385646 |
| Chain | Residue | Details |
| A | SER768 | |
| A | SER813 |
| site_id | SWS_FT_FI30 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PKC => ECO:0000269|PubMed:1377674 |
| Chain | Residue | Details |
| A | SER790 |
| site_id | SWS_FT_FI31 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:22119790 |
| Chain | Residue | Details |
| A | SER1444 | |
| A | SER1456 |
| site_id | SWS_FT_FI32 |
| Number of Residues | 2 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:22119790 |
| Chain | Residue | Details |
| A | CYS524 | |
| A | CYS1395 |
| site_id | SWS_FT_FI33 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:7518437, ECO:0000305|PubMed:20008117 |
| Chain | Residue | Details |
| A | ASN894 | |
| A | ASN900 |
| site_id | SWS_FT_FI34 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:22119790 |
| Chain | Residue | Details |
| A | LYS688 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 757 |
| Chain | Residue | Details |
| A | LEU167 | activator, proton acceptor, proton donor |
| A | SER169 | electrostatic stabiliser, polar interaction |
| A | LEU172 | metal ligand |
| A | SER185 | metal ligand |
| A | TRP202 | electrostatic stabiliser, polar interaction |
