9D8P
Focused map of Cryo-EM structure of Ubiquitin C-degron bound to KLHDC10-EloB/C
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0031462 | cellular_component | Cul2-RING ubiquitin ligase complex |
| A | 0032874 | biological_process | positive regulation of stress-activated MAPK cascade |
| A | 0072344 | biological_process | rescue of stalled ribosome |
| A | 0140627 | biological_process | ubiquitin-dependent protein catabolic process via the C-end degron rule pathway |
| A | 1990756 | molecular_function | ubiquitin-like ligase-substrate adaptor activity |
| B | 0000151 | cellular_component | ubiquitin ligase complex |
| B | 0001222 | molecular_function | transcription corepressor binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006367 | biological_process | transcription initiation at RNA polymerase II promoter |
| B | 0006368 | biological_process | transcription elongation by RNA polymerase II |
| B | 0016567 | biological_process | protein ubiquitination |
| B | 0030891 | cellular_component | VCB complex |
| B | 0031462 | cellular_component | Cul2-RING ubiquitin ligase complex |
| B | 0031466 | cellular_component | Cul5-RING ubiquitin ligase complex |
| B | 0031625 | molecular_function | ubiquitin protein ligase binding |
| B | 0032436 | biological_process | positive regulation of proteasomal ubiquitin-dependent protein catabolic process |
| B | 0065003 | biological_process | protein-containing complex assembly |
| B | 0070449 | cellular_component | elongin complex |
| B | 0140958 | biological_process | target-directed miRNA degradation |
| C | 0000151 | cellular_component | ubiquitin ligase complex |
| C | 0001222 | molecular_function | transcription corepressor binding |
| C | 0005515 | molecular_function | protein binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005654 | cellular_component | nucleoplasm |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006357 | biological_process | regulation of transcription by RNA polymerase II |
| C | 0006367 | biological_process | transcription initiation at RNA polymerase II promoter |
| C | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| C | 0016567 | biological_process | protein ubiquitination |
| C | 0030674 | molecular_function | protein-macromolecule adaptor activity |
| C | 0031462 | cellular_component | Cul2-RING ubiquitin ligase complex |
| C | 0031466 | cellular_component | Cul5-RING ubiquitin ligase complex |
| C | 0070449 | cellular_component | elongin complex |
| C | 0140958 | biological_process | target-directed miRNA degradation |
Functional Information from PROSITE/UniProt
| site_id | PS00299 |
| Number of Residues | 26 |
| Details | UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGcqleD |
| Chain | Residue | Details |
| D | LYS27-ASP52 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | SITE: Interacts with activating enzyme |
| Chain | Residue | Details |
| D | ARG54 | |
| D | ARG72 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | SITE: Essential for function |
| Chain | Residue | Details |
| D | HIS68 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291 |
| Chain | Residue | Details |
| D | SER65 | |
| B | SER111 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457 |
| Chain | Residue | Details |
| D | THR66 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742 |
| Chain | Residue | Details |
| D | GLY76 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 |
| Chain | Residue | Details |
| D | LYS6 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) |
| Chain | Residue | Details |
| D | GLY76 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 |
| Chain | Residue | Details |
| D | LYS11 | |
| D | CYS48 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860 |
| Chain | Residue | Details |
| D | LYS27 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127 |
| Chain | Residue | Details |
| D | LYS29 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577 |
| Chain | Residue | Details |
| D | LYS33 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106 |
| Chain | Residue | Details |
| D | LYS63 |
