Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

9D3I

Proteasome core particle assembly intermediate 5-alpha/4-beta/Ump1 purified from Saccharomyces cerevisiae.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000502	cellular_component	proteasome complex
A	0003674	molecular_function	molecular_function
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005839	cellular_component	proteasome core complex
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0030163	biological_process	protein catabolic process
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0000502	cellular_component	proteasome complex
B	0003674	molecular_function	molecular_function
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0030163	biological_process	protein catabolic process
B	0034515	cellular_component	proteasome storage granule
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0000502	cellular_component	proteasome complex
C	0003674	molecular_function	molecular_function
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0030163	biological_process	protein catabolic process
C	0034515	cellular_component	proteasome storage granule
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0080129	biological_process	proteasome core complex assembly
D	0000502	cellular_component	proteasome complex
D	0003674	molecular_function	molecular_function
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0030163	biological_process	protein catabolic process
D	0034515	cellular_component	proteasome storage granule
D	0042175	cellular_component	nuclear outer membrane-endoplasmic reticulum membrane network
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0000502	cellular_component	proteasome complex
G	0003729	molecular_function	mRNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005839	cellular_component	proteasome core complex
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0030163	biological_process	protein catabolic process
G	0034515	cellular_component	proteasome storage granule
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
H	0000502	cellular_component	proteasome complex
H	0004175	molecular_function	endopeptidase activity
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005839	cellular_component	proteasome core complex
H	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
H	0019774	cellular_component	proteasome core complex, beta-subunit complex
H	0030163	biological_process	protein catabolic process
H	0034515	cellular_component	proteasome storage granule
H	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	0000502	cellular_component	proteasome complex
I	0004175	molecular_function	endopeptidase activity
I	0004298	molecular_function	threonine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005839	cellular_component	proteasome core complex
I	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
I	0019774	cellular_component	proteasome core complex, beta-subunit complex
I	0030163	biological_process	protein catabolic process
I	0034515	cellular_component	proteasome storage granule
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0000502	cellular_component	proteasome complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0030163	biological_process	protein catabolic process
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0061133	molecular_function	endopeptidase activator activity
K	0000502	cellular_component	proteasome complex
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005789	cellular_component	endoplasmic reticulum membrane
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0010498	biological_process	proteasomal protein catabolic process
K	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
K	0019774	cellular_component	proteasome core complex, beta-subunit complex
K	0030163	biological_process	protein catabolic process
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0061133	molecular_function	endopeptidase activator activity
P	0003674	molecular_function	molecular_function
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0006974	biological_process	DNA damage response
P	0043248	biological_process	proteasome assembly
P	0090363	biological_process	obsolete regulation of proteasome core complex assembly

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdlsnSvFSPdGRnfQVEYAvkA

Chain	Residue	Details
G	TYR8-ALA30
B	TYR5-ALA27
A	TYR12-ALA34
D	TYR4-ALA26
C	TYR6-SER28

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LGIrVqdSVILASSkavtrgisvlkdsdd.KtrqlsphtlmsfaGeaGD

Chain	Residue	Details
K	LEU5-ASP52
J	VAL13-ASP59
I	VAL33-ASP80
H	MET4-ASP51
D	VAL34-ASP81
C	ILE36-ASP83

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18407956","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	10
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22106047","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17287358","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"9087403","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 177

Chain	Residue	Details
H	GLY11	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
H	ALA27	activator, steric locator
H	ARG29	activator
H	CYS43	activator, electrostatic stabiliser
H	ASP57	electrostatic stabiliser
H	ASP139	activator, electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)