9CHV
cryo-EM structure of calcineurin-fused beta2 adrenergic receptor in apo state
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001569 | biological_process | branching involved in blood vessel morphogenesis |
| A | 0001837 | biological_process | epithelial to mesenchymal transition |
| A | 0004721 | molecular_function | phosphoprotein phosphatase activity |
| A | 0004723 | molecular_function | calcium-dependent protein serine/threonine phosphatase activity |
| A | 0004930 | molecular_function | G protein-coupled receptor activity |
| A | 0004935 | molecular_function | adrenergic receptor activity |
| A | 0004941 | molecular_function | beta2-adrenergic receptor activity |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005516 | molecular_function | calmodulin binding |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005955 | cellular_component | calcineurin complex |
| A | 0006606 | biological_process | protein import into nucleus |
| A | 0006940 | biological_process | regulation of smooth muscle contraction |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| A | 0007507 | biological_process | heart development |
| A | 0008287 | cellular_component | protein serine/threonine phosphatase complex |
| A | 0008597 | molecular_function | calcium-dependent protein serine/threonine phosphatase regulator activity |
| A | 0014044 | biological_process | Schwann cell development |
| A | 0016020 | cellular_component | membrane |
| A | 0019902 | molecular_function | phosphatase binding |
| A | 0019904 | molecular_function | protein domain specific binding |
| A | 0022011 | biological_process | myelination in peripheral nervous system |
| A | 0033173 | biological_process | calcineurin-NFAT signaling cascade |
| A | 0034504 | biological_process | protein localization to nucleus |
| A | 0042383 | cellular_component | sarcolemma |
| A | 0045202 | cellular_component | synapse |
| A | 0045944 | biological_process | positive regulation of transcription by RNA polymerase II |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0060487 | biological_process | lung epithelial cell differentiation |
| A | 0070886 | biological_process | positive regulation of calcineurin-NFAT signaling cascade |
| A | 0097746 | biological_process | blood vessel diameter maintenance |
| A | 0098685 | cellular_component | Schaffer collateral - CA1 synapse |
| A | 0098686 | cellular_component | hippocampal mossy fiber to CA3 synapse |
| A | 0098688 | cellular_component | parallel fiber to Purkinje cell synapse |
| A | 0098693 | biological_process | regulation of synaptic vesicle cycle |
| A | 0098794 | cellular_component | postsynapse |
| A | 0098978 | cellular_component | glutamatergic synapse |
| A | 0099149 | biological_process | regulation of postsynaptic neurotransmitter receptor internalization |
| A | 0099170 | biological_process | postsynaptic modulation of chemical synaptic transmission |
| A | 1905665 | biological_process | positive regulation of calcium ion import across plasma membrane |
| A | 1905949 | biological_process | negative regulation of calcium ion import across plasma membrane |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0033192 | molecular_function | calmodulin-dependent protein phosphatase activity |
| B | 0097720 | biological_process | calcineurin-mediated signaling |
| C | 0003007 | biological_process | heart morphogenesis |
| C | 0003755 | molecular_function | peptidyl-prolyl cis-trans isomerase activity |
| C | 0005160 | molecular_function | transforming growth factor beta receptor binding |
| C | 0005246 | molecular_function | calcium channel regulator activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005527 | molecular_function | macrolide binding |
| C | 0005528 | molecular_function | FK506 binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006457 | biological_process | protein folding |
| C | 0006458 | biological_process | 'de novo' protein folding |
| C | 0010881 | biological_process | regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion |
| C | 0014802 | cellular_component | terminal cisterna |
| C | 0014809 | biological_process | regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion |
| C | 0016020 | cellular_component | membrane |
| C | 0016529 | cellular_component | sarcoplasmic reticulum |
| C | 0016853 | molecular_function | isomerase activity |
| C | 0030018 | cellular_component | Z disc |
| C | 0030512 | biological_process | negative regulation of transforming growth factor beta receptor signaling pathway |
| C | 0030547 | molecular_function | signaling receptor inhibitor activity |
| C | 0032880 | biological_process | regulation of protein localization |
| C | 0032926 | biological_process | negative regulation of activin receptor signaling pathway |
| C | 0033017 | cellular_component | sarcoplasmic reticulum membrane |
| C | 0034713 | molecular_function | type I transforming growth factor beta receptor binding |
| C | 0042026 | biological_process | protein refolding |
| C | 0042110 | biological_process | T cell activation |
| C | 0043123 | biological_process | positive regulation of canonical NF-kappaB signal transduction |
| C | 0044325 | molecular_function | transmembrane transporter binding |
| C | 0050776 | biological_process | regulation of immune response |
| C | 0051604 | biological_process | protein maturation |
| C | 0055010 | biological_process | ventricular cardiac muscle tissue morphogenesis |
| C | 0060347 | biological_process | heart trabecula formation |
| C | 0070411 | molecular_function | I-SMAD binding |
| C | 0070697 | molecular_function | activin receptor binding |
| C | 0097435 | biological_process | supramolecular fiber organization |
| C | 0098562 | cellular_component | cytoplasmic side of membrane |
| C | 1902991 | biological_process | regulation of amyloid precursor protein catabolic process |
| C | 1990000 | biological_process | amyloid fibril formation |
| C | 1990425 | cellular_component | ryanodine receptor complex |
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DLDNSGSLSveEF |
| Chain | Residue | Details |
| A | ASP246-PHE258 | |
| A | ASP278-PHE290 | |
| A | ASP315-LEU327 | |
| A | ASP356-PHE368 |
| site_id | PS00125 |
| Number of Residues | 6 |
| Details | SER_THR_PHOSPHATASE Serine/threonine specific protein phosphatases signature. LRGNHE |
| Chain | Residue | Details |
| B | LEU146-GLU151 |
| site_id | PS00237 |
| Number of Residues | 17 |
| Details | G_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ASIeTLCVIAVDRYFaI |
| Chain | Residue | Details |
| A | ALA119-ILE135 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P26883 |
| Chain | Residue | Details |
| C | LYS52 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:26794871, ECO:0007744|PDB:4ORB |
| Chain | Residue | Details |
| B | ASP89 | |
| B | HIS91 | |
| B | ASP117 | |
| B | ASN149 | |
| B | HIS198 | |
| B | HIS280 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | SITE: Interaction with PxVP motif in substrate => ECO:0000250|UniProtKB:Q08209 |
| Chain | Residue | Details |
| B | TRP351 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylserine => ECO:0000250|UniProtKB:Q08209 |
| Chain | Residue | Details |
| B | SER1 | |
| A | ARG175-ASN196 | |
| A | GLN422-LYS428 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: 3'-nitrotyrosine => ECO:0007744|PubMed:16800626 |
| Chain | Residue | Details |
| B | TYR223 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=4 |
| Chain | Residue | Details |
| A | ARG151-TYR174 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=5 |
| Chain | Residue | Details |
| A | GLN197-SER220 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=6 |
| Chain | Residue | Details |
| A | LEU398-ILE421 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=7 |
| Chain | Residue | Details |
| A | GLU429-SER452 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:18547522, ECO:0007744|PDB:3D4S |
| Chain | Residue | Details |
| A | ASP113 | |
| A | THR118 | |
| A | ASN416 | |
| A | ASN435 | |
| A | TYR439 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17952055, ECO:0000269|PubMed:17962520, ECO:0007744|PDB:2RH1 |
| Chain | Residue | Details |
| A | SER203 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:8521811 |
| Chain | Residue | Details |
| A | TYR141 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:11146000 |
| Chain | Residue | Details |
| A | ASP468 | |
| A | ASP469 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:27481942 |
| Chain | Residue | Details |
| A | CYS388 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:17962520, ECO:0000269|PubMed:18547522, ECO:0000269|PubMed:2540197, ECO:0000269|PubMed:27481942 |
| Chain | Residue | Details |
| A | CYS464 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 15 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:12218175, ECO:0000269|PubMed:12357034, ECO:0000269|PubMed:17498738, ECO:0000269|PubMed:22343722, ECO:0000269|PubMed:23468591, ECO:0000269|PubMed:26794871, ECO:0000269|PubMed:27974827, ECO:0000269|PubMed:8524402, ECO:0007744|PDB:1AUI, ECO:0007744|PDB:1M63, ECO:0007744|PDB:1MF8, ECO:0007744|PDB:2P6B, ECO:0007744|PDB:3LL8, ECO:0007744|PDB:4F0Z, ECO:0007744|PDB:4OR9, ECO:0007744|PDB:4ORA, ECO:0007744|PDB:4ORC, ECO:0007744|PDB:5SVE |
| Chain | Residue | Details |
| A | ASP246 | |
| A | GLU289 | |
| A | ASP356 | |
| A | ASP358 | |
| A | ASP360 | |
| A | ARG362 | |
| A | GLU367 | |
| A | ASP248 | |
| A | SER250 | |
| A | SER252 | |
| A | GLU257 | |
| A | ASP278 | |
| A | ASP280 | |
| A | ASN282 | |
| A | GLU284 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:12218175, ECO:0000269|PubMed:12357034, ECO:0000269|PubMed:17498738, ECO:0000269|PubMed:22343722, ECO:0000269|PubMed:23468591, ECO:0000269|PubMed:26794871, ECO:0000269|PubMed:27974827, ECO:0000269|PubMed:31375679, ECO:0000269|PubMed:8524402, ECO:0000312|PDB:6NUC, ECO:0007744|PDB:1AUI, ECO:0007744|PDB:1M63, ECO:0007744|PDB:1MF8, ECO:0007744|PDB:2P6B, ECO:0007744|PDB:3LL8, ECO:0007744|PDB:4F0Z, ECO:0007744|PDB:4OR9, ECO:0007744|PDB:4ORA, ECO:0007744|PDB:4ORC, ECO:0007744|PDB:5SVE |
| Chain | Residue | Details |
| A | ASP315 | |
| A | ASP317 | |
| A | ASP319 | |
| A | TYR321 | |
| A | GLU326 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | SITE: Interaction with PxVP motif in substrates of the catalytic subunit => ECO:0000269|PubMed:23468591 |
| Chain | Residue | Details |
| A | MET333 | |
| A | ASN337 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:Q63810 |
| Chain | Residue | Details |
| A | TYR321 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 362 |
| Chain | Residue | Details |
| C | TYR26 | electrostatic destabiliser, steric role |
| C | PHE36 | electrostatic destabiliser, polar/non-polar interaction, steric role |
| C | ASP37 | electrostatic stabiliser, steric role |
| C | ILE56 | electrostatic stabiliser, steric role |
| C | TYR82 | electrostatic stabiliser, steric role |
| C | PHE99 | electrostatic destabiliser, polar/non-polar interaction, steric role |
