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9CFX

Cryo-EM structure of myosin-1c bound to F-actin in the Rigor state

Functional Information from GO Data
ChainGOidnamespacecontents
B0000166molecular_functionnucleotide binding
B0000287molecular_functionmagnesium ion binding
B0001725cellular_componentstress fiber
B0003785molecular_functionactin monomer binding
B0005509molecular_functioncalcium ion binding
B0005515molecular_functionprotein binding
B0005523molecular_functiontropomyosin binding
B0005524molecular_functionATP binding
B0005737cellular_componentcytoplasm
B0005856cellular_componentcytoskeleton
B0005865cellular_componentstriated muscle thin filament
B0005884cellular_componentactin filament
B0010628biological_processpositive regulation of gene expression
B0016787molecular_functionhydrolase activity
B0019904molecular_functionprotein domain specific binding
B0030027cellular_componentlamellipodium
B0030041biological_processactin filament polymerization
B0030175cellular_componentfilopodium
B0030240biological_processskeletal muscle thin filament assembly
B0031013molecular_functiontroponin I binding
B0031432molecular_functiontitin binding
B0031941cellular_componentfilamentous actin
B0032036molecular_functionmyosin heavy chain binding
B0032432cellular_componentactin filament bundle
B0042802molecular_functionidentical protein binding
B0044297cellular_componentcell body
B0048306molecular_functioncalcium-dependent protein binding
B0048741biological_processskeletal muscle fiber development
B0051017biological_processactin filament bundle assembly
B0090131biological_processmesenchyme migration
B0098723cellular_componentskeletal muscle myofibril
B0140660molecular_functioncytoskeletal motor activator activity
C0000166molecular_functionnucleotide binding
C0000287molecular_functionmagnesium ion binding
C0001725cellular_componentstress fiber
C0003785molecular_functionactin monomer binding
C0005509molecular_functioncalcium ion binding
C0005515molecular_functionprotein binding
C0005523molecular_functiontropomyosin binding
C0005524molecular_functionATP binding
C0005737cellular_componentcytoplasm
C0005856cellular_componentcytoskeleton
C0005865cellular_componentstriated muscle thin filament
C0005884cellular_componentactin filament
C0010628biological_processpositive regulation of gene expression
C0016787molecular_functionhydrolase activity
C0019904molecular_functionprotein domain specific binding
C0030027cellular_componentlamellipodium
C0030041biological_processactin filament polymerization
C0030175cellular_componentfilopodium
C0030240biological_processskeletal muscle thin filament assembly
C0031013molecular_functiontroponin I binding
C0031432molecular_functiontitin binding
C0031941cellular_componentfilamentous actin
C0032036molecular_functionmyosin heavy chain binding
C0032432cellular_componentactin filament bundle
C0042802molecular_functionidentical protein binding
C0044297cellular_componentcell body
C0048306molecular_functioncalcium-dependent protein binding
C0048741biological_processskeletal muscle fiber development
C0051017biological_processactin filament bundle assembly
C0090131biological_processmesenchyme migration
C0098723cellular_componentskeletal muscle myofibril
C0140660molecular_functioncytoskeletal motor activator activity
D0000166molecular_functionnucleotide binding
D0000287molecular_functionmagnesium ion binding
D0001725cellular_componentstress fiber
D0003785molecular_functionactin monomer binding
D0005509molecular_functioncalcium ion binding
D0005515molecular_functionprotein binding
D0005523molecular_functiontropomyosin binding
D0005524molecular_functionATP binding
D0005737cellular_componentcytoplasm
D0005856cellular_componentcytoskeleton
D0005865cellular_componentstriated muscle thin filament
D0005884cellular_componentactin filament
D0010628biological_processpositive regulation of gene expression
D0016787molecular_functionhydrolase activity
D0019904molecular_functionprotein domain specific binding
D0030027cellular_componentlamellipodium
D0030041biological_processactin filament polymerization
D0030175cellular_componentfilopodium
D0030240biological_processskeletal muscle thin filament assembly
D0031013molecular_functiontroponin I binding
D0031432molecular_functiontitin binding
D0031941cellular_componentfilamentous actin
D0032036molecular_functionmyosin heavy chain binding
D0032432cellular_componentactin filament bundle
D0042802molecular_functionidentical protein binding
D0044297cellular_componentcell body
D0048306molecular_functioncalcium-dependent protein binding
D0048741biological_processskeletal muscle fiber development
D0051017biological_processactin filament bundle assembly
D0090131biological_processmesenchyme migration
D0098723cellular_componentskeletal muscle myofibril
D0140660molecular_functioncytoskeletal motor activator activity
P0003774molecular_functioncytoskeletal motor activity
P0005524molecular_functionATP binding
P0016459cellular_componentmyosin complex
R0000086biological_processG2/M transition of mitotic cell cycle
R0000922cellular_componentspindle pole
R0001975biological_processresponse to amphetamine
R0002027biological_processregulation of heart rate
R0005246molecular_functioncalcium channel regulator activity
R0005509molecular_functioncalcium ion binding
R0005513biological_processdetection of calcium ion
R0005515molecular_functionprotein binding
R0005654cellular_componentnucleoplasm
R0005737cellular_componentcytoplasm
R0005739cellular_componentmitochondrion
R0005813cellular_componentcentrosome
R0005819cellular_componentspindle
R0005829cellular_componentcytosol
R0005856cellular_componentcytoskeleton
R0005876cellular_componentspindle microtubule
R0005929cellular_componentcilium
R0006897biological_processendocytosis
R0008076cellular_componentvoltage-gated potassium channel complex
R0008179molecular_functionadenylate cyclase binding
R0010856molecular_functionadenylate cyclase activator activity
R0010880biological_processregulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
R0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
R0016240biological_processautophagosome membrane docking
R0019855molecular_functioncalcium channel inhibitor activity
R0019901molecular_functionprotein kinase binding
R0019904molecular_functionprotein domain specific binding
R0030017cellular_componentsarcomere
R0030235molecular_functionnitric-oxide synthase regulator activity
R0030426cellular_componentgrowth cone
R0030672cellular_componentsynaptic vesicle membrane
R0031432molecular_functiontitin binding
R0031514cellular_componentmotile cilium
R0031800molecular_functiontype 3 metabotropic glutamate receptor binding
R0031966cellular_componentmitochondrial membrane
R0032465biological_processregulation of cytokinesis
R0032991cellular_componentprotein-containing complex
R0034704cellular_componentcalcium channel complex
R0035458biological_processcellular response to interferon-beta
R0042995cellular_componentcell projection
R0043209cellular_componentmyelin sheath
R0043539molecular_functionprotein serine/threonine kinase activator activity
R0043548molecular_functionphosphatidylinositol 3-kinase binding
R0044305cellular_componentcalyx of Held
R0044325molecular_functiontransmembrane transporter binding
R0046427biological_processpositive regulation of receptor signaling pathway via JAK-STAT
R0046872molecular_functionmetal ion binding
R0048306molecular_functioncalcium-dependent protein binding
R0050998molecular_functionnitric-oxide synthase binding
R0051412biological_processresponse to corticosterone
R0051592biological_processresponse to calcium ion
R0051649biological_processestablishment of localization in cell
R0055117biological_processregulation of cardiac muscle contraction
R0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
R0060315biological_processnegative regulation of ryanodine-sensitive calcium-release channel activity
R0071346biological_processcellular response to type II interferon
R0072542molecular_functionprotein phosphatase activator activity
R0090150biological_processestablishment of protein localization to membrane
R0090151biological_processestablishment of protein localization to mitochondrial membrane
R0097225cellular_componentsperm midpiece
R0097720biological_processcalcineurin-mediated signaling
R0098685cellular_componentSchaffer collateral - CA1 synapse
R0098901biological_processregulation of cardiac muscle cell action potential
R0099523cellular_componentpresynaptic cytosol
R0099524cellular_componentpostsynaptic cytosol
R0140056biological_processorganelle localization by membrane tethering
R0140238biological_processpresynaptic endocytosis
R0150034cellular_componentdistal axon
R1900242biological_processregulation of synaptic vesicle endocytosis
R1902494cellular_componentcatalytic complex
R1990456biological_processmitochondrion-endoplasmic reticulum membrane tethering
R2000300biological_processregulation of synaptic vesicle exocytosis
Functional Information from PROSITE/UniProt
site_idPS00018
Number of Residues13
DetailsEF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL
ChainResidueDetails
RASP21-LEU33
RASP57-PHE69
RASP94-LEU106
RASP130-PHE142

site_idPS00406
Number of Residues11
DetailsACTINS_1 Actins signature 1. YVGDEAQs.KRG
ChainResidueDetails
BTYR53-GLY63

site_idPS00432
Number of Residues9
DetailsACTINS_2 Actins signature 2. WITKqEYDE
ChainResidueDetails
BTRP356-GLU364

site_idPS01132
Number of Residues13
DetailsACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR
ChainResidueDetails
BLEU104-ARG116

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues20
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0007744|PDB:4HEX, ECO:0007744|PDB:4ZLK
ChainResidueDetails
RASP21
RGLU68
RASP94
RASP96
RASN98
RTYR100
RGLU105
RASP130
RASP132
RASP134
RGLN136
RASP23
RGLU141
RASP25
RTHR27
RGLU32
RASP57
RASP59
RASN61
RTHR63

site_idSWS_FT_FI2
Number of Residues1
DetailsMOD_RES: N-acetylalanine => ECO:0000269|Ref.5
ChainResidueDetails
RALA2
BMET47
CMET44
CMET47
DMET44
DMET47

site_idSWS_FT_FI3
Number of Residues1
DetailsMOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:23806337
ChainResidueDetails
RLYS22
CLYS61
DLYS61

site_idSWS_FT_FI4
Number of Residues1
DetailsMOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29
ChainResidueDetails
RTHR45
CHIS73
DHIS73

site_idSWS_FT_FI5
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
RSER82
CLYS84
DLYS84

site_idSWS_FT_FI6
Number of Residues1
DetailsMOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
RLYS95
CARG177
DARG177

site_idSWS_FT_FI7
Number of Residues1
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18034455, ECO:0007744|PubMed:21183079
ChainResidueDetails
RTYR100

site_idSWS_FT_FI8
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079
ChainResidueDetails
RSER102

site_idSWS_FT_FI9
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
RTHR111

site_idSWS_FT_FI10
Number of Residues1
DetailsMOD_RES: N6-methyllysine; alternate => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
RLYS116

site_idSWS_FT_FI11
Number of Residues1
DetailsMOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P0DP23
ChainResidueDetails
RTYR139

site_idSWS_FT_FI12
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157
ChainResidueDetails
RLYS22

236620

PDB entries from 2025-05-28

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