9B5G
Ubiquitin E1-Ub-E2 tetrahedral transthiolation intermediate mimic (doubly Ub-loaded) - cluster 2 map and model (Ub(A)/ATP/Mg)
This is a non-PDB format compatible entry.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0004839 | molecular_function | ubiquitin activating enzyme activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| A | 0006974 | biological_process | DNA damage response |
| A | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0016874 | molecular_function | ligase activity |
| A | 0016887 | molecular_function | ATP hydrolysis activity |
| A | 0036211 | biological_process | protein modification process |
| A | 0046872 | molecular_function | metal ion binding |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0000209 | biological_process | protein polyubiquitination |
| C | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| C | 0016567 | biological_process | protein ubiquitination |
| C | 0016740 | molecular_function | transferase activity |
| C | 0031146 | biological_process | SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
| C | 0032933 | biological_process | SREBP signaling pathway |
| C | 0045842 | biological_process | positive regulation of mitotic metaphase/anaphase transition |
| C | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
Functional Information from PROSITE/UniProt
| site_id | PS00183 |
| Number of Residues | 16 |
| Details | UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNInsn.GsICLdiL |
| Chain | Residue | Details |
| C | TYR74-LEU89 |
| site_id | PS00299 |
| Number of Residues | 26 |
| Details | UBIQUITIN_1 Ubiquitin domain signature. KskIqDkegIPpdqQrLIFaGkqleD |
| Chain | Residue | Details |
| D | LYS105-ASP130 | |
| B | LYS27-ASP52 |
| site_id | PS00865 |
| Number of Residues | 9 |
| Details | UBIQUITIN_ACTIVAT_2 Ubiquitin-activating enzyme active site. PICTLKnFP |
| Chain | Residue | Details |
| A | PRO591-PRO599 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) |
| Chain | Residue | Details |
| D | LYS84 | |
| D | LYS89 | |
| D | LYS105 | |
| D | LYS111 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250 |
| Chain | Residue | Details |
| A | ARG22 | |
| D | LYS107 | |
| D | LYS126 | |
| D | LYS141 | |
| A | VAL513 | |
| A | ASN538 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250 |
| Chain | Residue | Details |
| B | LYS29 | |
| B | LYS48 | |
| B | LYS63 | |
| A | ASP537 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:23416107, ECO:0007744|PDB:4II2 |
| Chain | Residue | Details |
| A | ARG474 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P22515 |
| Chain | Residue | Details |
| A | SER264 | |
| A | SER903 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P22515 |
| Chain | Residue | Details |
| A | LYS588 |
