9ATK

BIFUNCTIONAL INHIBITION OF NEUTROPHIL ELASTASE AND CATHEPSIN G by Eap4 of S. aureus

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0002020	molecular_function	protease binding
A	0002438	biological_process	acute inflammatory response to antigenic stimulus
A	0002523	biological_process	leukocyte migration involved in inflammatory response
A	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
A	0003714	molecular_function	transcription corepressor activity
A	0004175	molecular_function	endopeptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006508	biological_process	proteolysis
A	0006874	biological_process	intracellular calcium ion homeostasis
A	0006909	biological_process	phagocytosis
A	0008201	molecular_function	heparin binding
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0009411	biological_process	response to UV
A	0009986	cellular_component	cell surface
A	0016787	molecular_function	hydrolase activity
A	0017053	cellular_component	transcription repressor complex
A	0019955	molecular_function	cytokine binding
A	0022617	biological_process	extracellular matrix disassembly
A	0030141	cellular_component	secretory granule
A	0030163	biological_process	protein catabolic process
A	0031012	cellular_component	extracellular matrix
A	0031410	cellular_component	cytoplasmic vesicle
A	0031452	biological_process	negative regulation of heterochromatin formation
A	0032682	biological_process	negative regulation of chemokine production
A	0032717	biological_process	negative regulation of interleukin-8 production
A	0032757	biological_process	positive regulation of interleukin-8 production
A	0035578	cellular_component	azurophil granule lumen
A	0035580	cellular_component	specific granule lumen
A	0042582	cellular_component	azurophil granule
A	0042742	biological_process	defense response to bacterium
A	0043406	biological_process	positive regulation of MAP kinase activity
A	0045335	cellular_component	phagocytic vesicle
A	0048661	biological_process	positive regulation of smooth muscle cell proliferation
A	0050728	biological_process	negative regulation of inflammatory response
A	0050922	biological_process	negative regulation of chemotaxis
A	0070062	cellular_component	extracellular exosome
A	0070269	biological_process	pyroptotic inflammatory response
A	0070944	biological_process	neutrophil-mediated killing of bacterium
A	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
A	0070947	biological_process	neutrophil-mediated killing of fungus
A	0140644	cellular_component	neutrophil extracellular trap
A	0140645	biological_process	neutrophil extracellular trap formation
A	1903238	biological_process	positive regulation of leukocyte tethering or rolling
B	0002003	biological_process	angiotensin maturation
B	0002548	biological_process	monocyte chemotaxis
B	0004252	molecular_function	serine-type endopeptidase activity
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005634	cellular_component	nucleus
B	0005764	cellular_component	lysosome
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006508	biological_process	proteolysis
B	0006935	biological_process	chemotaxis
B	0006955	biological_process	immune response
B	0008201	molecular_function	heparin binding
B	0008233	molecular_function	peptidase activity
B	0008236	molecular_function	serine-type peptidase activity
B	0010494	cellular_component	cytoplasmic stress granule
B	0016020	cellular_component	membrane
B	0016485	biological_process	protein processing
B	0016787	molecular_function	hydrolase activity
B	0019221	biological_process	cytokine-mediated signaling pathway
B	0019731	biological_process	antibacterial humoral response
B	0022617	biological_process	extracellular matrix disassembly
B	0030141	cellular_component	secretory granule
B	0030168	biological_process	platelet activation
B	0031012	cellular_component	extracellular matrix
B	0035578	cellular_component	azurophil granule lumen
B	0035590	biological_process	purinergic nucleotide receptor signaling pathway
B	0042119	biological_process	neutrophil activation
B	0042742	biological_process	defense response to bacterium
B	0048018	molecular_function	receptor ligand activity
B	0050829	biological_process	defense response to Gram-negative bacterium
B	0050830	biological_process	defense response to Gram-positive bacterium
B	0050868	biological_process	negative regulation of T cell activation
B	0051604	biological_process	protein maturation
B	0070062	cellular_component	extracellular exosome
B	0071222	biological_process	cellular response to lipopolysaccharide
B	0089720	molecular_function	caspase binding
B	0098786	biological_process	biofilm matrix disassembly
B	1901731	biological_process	positive regulation of platelet aggregation
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0002020	molecular_function	protease binding
D	0002438	biological_process	acute inflammatory response to antigenic stimulus
D	0002523	biological_process	leukocyte migration involved in inflammatory response
D	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
D	0003714	molecular_function	transcription corepressor activity
D	0004175	molecular_function	endopeptidase activity
D	0004252	molecular_function	serine-type endopeptidase activity
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006508	biological_process	proteolysis
D	0006874	biological_process	intracellular calcium ion homeostasis
D	0006909	biological_process	phagocytosis
D	0008201	molecular_function	heparin binding
D	0008233	molecular_function	peptidase activity
D	0008236	molecular_function	serine-type peptidase activity
D	0009411	biological_process	response to UV
D	0009986	cellular_component	cell surface
D	0016787	molecular_function	hydrolase activity
D	0017053	cellular_component	transcription repressor complex
D	0019955	molecular_function	cytokine binding
D	0022617	biological_process	extracellular matrix disassembly
D	0030141	cellular_component	secretory granule
D	0030163	biological_process	protein catabolic process
D	0031012	cellular_component	extracellular matrix
D	0031410	cellular_component	cytoplasmic vesicle
D	0031452	biological_process	negative regulation of heterochromatin formation
D	0032682	biological_process	negative regulation of chemokine production
D	0032717	biological_process	negative regulation of interleukin-8 production
D	0032757	biological_process	positive regulation of interleukin-8 production
D	0035578	cellular_component	azurophil granule lumen
D	0035580	cellular_component	specific granule lumen
D	0042582	cellular_component	azurophil granule
D	0042742	biological_process	defense response to bacterium
D	0043406	biological_process	positive regulation of MAP kinase activity
D	0045335	cellular_component	phagocytic vesicle
D	0048661	biological_process	positive regulation of smooth muscle cell proliferation
D	0050728	biological_process	negative regulation of inflammatory response
D	0050922	biological_process	negative regulation of chemotaxis
D	0070062	cellular_component	extracellular exosome
D	0070269	biological_process	pyroptotic inflammatory response
D	0070944	biological_process	neutrophil-mediated killing of bacterium
D	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
D	0070947	biological_process	neutrophil-mediated killing of fungus
D	0140644	cellular_component	neutrophil extracellular trap
D	0140645	biological_process	neutrophil extracellular trap formation
D	1903238	biological_process	positive regulation of leukocyte tethering or rolling
E	0002003	biological_process	angiotensin maturation
E	0002548	biological_process	monocyte chemotaxis
E	0004252	molecular_function	serine-type endopeptidase activity
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0005634	cellular_component	nucleus
E	0005764	cellular_component	lysosome
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006508	biological_process	proteolysis
E	0006935	biological_process	chemotaxis
E	0006955	biological_process	immune response
E	0008201	molecular_function	heparin binding
E	0008233	molecular_function	peptidase activity
E	0008236	molecular_function	serine-type peptidase activity
E	0010494	cellular_component	cytoplasmic stress granule
E	0016020	cellular_component	membrane
E	0016485	biological_process	protein processing
E	0016787	molecular_function	hydrolase activity
E	0019221	biological_process	cytokine-mediated signaling pathway
E	0019731	biological_process	antibacterial humoral response
E	0022617	biological_process	extracellular matrix disassembly
E	0030141	cellular_component	secretory granule
E	0030168	biological_process	platelet activation
E	0031012	cellular_component	extracellular matrix
E	0035578	cellular_component	azurophil granule lumen
E	0035590	biological_process	purinergic nucleotide receptor signaling pathway
E	0042119	biological_process	neutrophil activation
E	0042742	biological_process	defense response to bacterium
E	0048018	molecular_function	receptor ligand activity
E	0050829	biological_process	defense response to Gram-negative bacterium
E	0050830	biological_process	defense response to Gram-positive bacterium
E	0050868	biological_process	negative regulation of T cell activation
E	0051604	biological_process	protein maturation
E	0070062	cellular_component	extracellular exosome
E	0071222	biological_process	cellular response to lipopolysaccharide
E	0089720	molecular_function	caspase binding
E	0098786	biological_process	biofilm matrix disassembly
E	1901731	biological_process	positive regulation of platelet aggregation
G	0000122	biological_process	negative regulation of transcription by RNA polymerase II
G	0002020	molecular_function	protease binding
G	0002438	biological_process	acute inflammatory response to antigenic stimulus
G	0002523	biological_process	leukocyte migration involved in inflammatory response
G	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
G	0003714	molecular_function	transcription corepressor activity
G	0004175	molecular_function	endopeptidase activity
G	0004252	molecular_function	serine-type endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005615	cellular_component	extracellular space
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006508	biological_process	proteolysis
G	0006874	biological_process	intracellular calcium ion homeostasis
G	0006909	biological_process	phagocytosis
G	0008201	molecular_function	heparin binding
G	0008233	molecular_function	peptidase activity
G	0008236	molecular_function	serine-type peptidase activity
G	0009411	biological_process	response to UV
G	0009986	cellular_component	cell surface
G	0016787	molecular_function	hydrolase activity
G	0017053	cellular_component	transcription repressor complex
G	0019955	molecular_function	cytokine binding
G	0022617	biological_process	extracellular matrix disassembly
G	0030141	cellular_component	secretory granule
G	0030163	biological_process	protein catabolic process
G	0031012	cellular_component	extracellular matrix
G	0031410	cellular_component	cytoplasmic vesicle
G	0031452	biological_process	negative regulation of heterochromatin formation
G	0032682	biological_process	negative regulation of chemokine production
G	0032717	biological_process	negative regulation of interleukin-8 production
G	0032757	biological_process	positive regulation of interleukin-8 production
G	0035578	cellular_component	azurophil granule lumen
G	0035580	cellular_component	specific granule lumen
G	0042582	cellular_component	azurophil granule
G	0042742	biological_process	defense response to bacterium
G	0043406	biological_process	positive regulation of MAP kinase activity
G	0045335	cellular_component	phagocytic vesicle
G	0048661	biological_process	positive regulation of smooth muscle cell proliferation
G	0050728	biological_process	negative regulation of inflammatory response
G	0050922	biological_process	negative regulation of chemotaxis
G	0070062	cellular_component	extracellular exosome
G	0070269	biological_process	pyroptotic inflammatory response
G	0070944	biological_process	neutrophil-mediated killing of bacterium
G	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
G	0070947	biological_process	neutrophil-mediated killing of fungus
G	0140644	cellular_component	neutrophil extracellular trap
G	0140645	biological_process	neutrophil extracellular trap formation
G	1903238	biological_process	positive regulation of leukocyte tethering or rolling
H	0002003	biological_process	angiotensin maturation
H	0002548	biological_process	monocyte chemotaxis
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005615	cellular_component	extracellular space
H	0005634	cellular_component	nucleus
H	0005764	cellular_component	lysosome
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0006508	biological_process	proteolysis
H	0006935	biological_process	chemotaxis
H	0006955	biological_process	immune response
H	0008201	molecular_function	heparin binding
H	0008233	molecular_function	peptidase activity
H	0008236	molecular_function	serine-type peptidase activity
H	0010494	cellular_component	cytoplasmic stress granule
H	0016020	cellular_component	membrane
H	0016485	biological_process	protein processing
H	0016787	molecular_function	hydrolase activity
H	0019221	biological_process	cytokine-mediated signaling pathway
H	0019731	biological_process	antibacterial humoral response
H	0022617	biological_process	extracellular matrix disassembly
H	0030141	cellular_component	secretory granule
H	0030168	biological_process	platelet activation
H	0031012	cellular_component	extracellular matrix
H	0035578	cellular_component	azurophil granule lumen
H	0035590	biological_process	purinergic nucleotide receptor signaling pathway
H	0042119	biological_process	neutrophil activation
H	0042742	biological_process	defense response to bacterium
H	0048018	molecular_function	receptor ligand activity
H	0050829	biological_process	defense response to Gram-negative bacterium
H	0050830	biological_process	defense response to Gram-positive bacterium
H	0050868	biological_process	negative regulation of T cell activation
H	0051604	biological_process	protein maturation
H	0070062	cellular_component	extracellular exosome
H	0071222	biological_process	cellular response to lipopolysaccharide
H	0089720	molecular_function	caspase binding
H	0098786	biological_process	biofilm matrix disassembly
H	1901731	biological_process	positive regulation of platelet aggregation
J	0000122	biological_process	negative regulation of transcription by RNA polymerase II
J	0002020	molecular_function	protease binding
J	0002438	biological_process	acute inflammatory response to antigenic stimulus
J	0002523	biological_process	leukocyte migration involved in inflammatory response
J	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
J	0003714	molecular_function	transcription corepressor activity
J	0004175	molecular_function	endopeptidase activity
J	0004252	molecular_function	serine-type endopeptidase activity
J	0005515	molecular_function	protein binding
J	0005576	cellular_component	extracellular region
J	0005615	cellular_component	extracellular space
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006508	biological_process	proteolysis
J	0006874	biological_process	intracellular calcium ion homeostasis
J	0006909	biological_process	phagocytosis
J	0008201	molecular_function	heparin binding
J	0008233	molecular_function	peptidase activity
J	0008236	molecular_function	serine-type peptidase activity
J	0009411	biological_process	response to UV
J	0009986	cellular_component	cell surface
J	0016787	molecular_function	hydrolase activity
J	0017053	cellular_component	transcription repressor complex
J	0019955	molecular_function	cytokine binding
J	0022617	biological_process	extracellular matrix disassembly
J	0030141	cellular_component	secretory granule
J	0030163	biological_process	protein catabolic process
J	0031012	cellular_component	extracellular matrix
J	0031410	cellular_component	cytoplasmic vesicle
J	0031452	biological_process	negative regulation of heterochromatin formation
J	0032682	biological_process	negative regulation of chemokine production
J	0032717	biological_process	negative regulation of interleukin-8 production
J	0032757	biological_process	positive regulation of interleukin-8 production
J	0035578	cellular_component	azurophil granule lumen
J	0035580	cellular_component	specific granule lumen
J	0042582	cellular_component	azurophil granule
J	0042742	biological_process	defense response to bacterium
J	0043406	biological_process	positive regulation of MAP kinase activity
J	0045335	cellular_component	phagocytic vesicle
J	0048661	biological_process	positive regulation of smooth muscle cell proliferation
J	0050728	biological_process	negative regulation of inflammatory response
J	0050922	biological_process	negative regulation of chemotaxis
J	0070062	cellular_component	extracellular exosome
J	0070269	biological_process	pyroptotic inflammatory response
J	0070944	biological_process	neutrophil-mediated killing of bacterium
J	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
J	0070947	biological_process	neutrophil-mediated killing of fungus
J	0140644	cellular_component	neutrophil extracellular trap
J	0140645	biological_process	neutrophil extracellular trap formation
J	1903238	biological_process	positive regulation of leukocyte tethering or rolling
K	0002003	biological_process	angiotensin maturation
K	0002548	biological_process	monocyte chemotaxis
K	0004252	molecular_function	serine-type endopeptidase activity
K	0005515	molecular_function	protein binding
K	0005576	cellular_component	extracellular region
K	0005615	cellular_component	extracellular space
K	0005634	cellular_component	nucleus
K	0005764	cellular_component	lysosome
K	0005829	cellular_component	cytosol
K	0005886	cellular_component	plasma membrane
K	0006508	biological_process	proteolysis
K	0006935	biological_process	chemotaxis
K	0006955	biological_process	immune response
K	0008201	molecular_function	heparin binding
K	0008233	molecular_function	peptidase activity
K	0008236	molecular_function	serine-type peptidase activity
K	0010494	cellular_component	cytoplasmic stress granule
K	0016020	cellular_component	membrane
K	0016485	biological_process	protein processing
K	0016787	molecular_function	hydrolase activity
K	0019221	biological_process	cytokine-mediated signaling pathway
K	0019731	biological_process	antibacterial humoral response
K	0022617	biological_process	extracellular matrix disassembly
K	0030141	cellular_component	secretory granule
K	0030168	biological_process	platelet activation
K	0031012	cellular_component	extracellular matrix
K	0035578	cellular_component	azurophil granule lumen
K	0035590	biological_process	purinergic nucleotide receptor signaling pathway
K	0042119	biological_process	neutrophil activation
K	0042742	biological_process	defense response to bacterium
K	0048018	molecular_function	receptor ligand activity
K	0050829	biological_process	defense response to Gram-negative bacterium
K	0050830	biological_process	defense response to Gram-positive bacterium
K	0050868	biological_process	negative regulation of T cell activation
K	0051604	biological_process	protein maturation
K	0070062	cellular_component	extracellular exosome
K	0071222	biological_process	cellular response to lipopolysaccharide
K	0089720	molecular_function	caspase binding
K	0098786	biological_process	biofilm matrix disassembly
K	1901731	biological_process	positive regulation of platelet aggregation

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. LTAAHC

Chain	Residue	Details
E	LEU55-CYS60
A	MET65-CYS70

---

site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. AAfkGDSGGPLL

Chain	Residue	Details
E	ALA190-LEU201
A	GLY195-VAL206

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1756
Details	Domain: {"description":"Peptidase S1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"2911584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"26274980","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3550808","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3550808","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	72
Details	Region: {"description":"Important for antimicrobial activity","evidences":[{"source":"PubMed","id":"2116408","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) (paucimannose) asparagine; alternate","evidences":[{"source":"PubMed","id":"26274980","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---