Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

9ASM

Human Drosha and DGCR8 in complex with Pri-let-7f1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001530	molecular_function	lipopolysaccharide binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0004525	molecular_function	ribonuclease III activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006364	biological_process	rRNA processing
A	0006396	biological_process	RNA processing
A	0010468	biological_process	regulation of gene expression
A	0010586	biological_process	miRNA metabolic process
A	0010628	biological_process	positive regulation of gene expression
A	0014069	cellular_component	postsynaptic density
A	0016787	molecular_function	hydrolase activity
A	0017151	molecular_function	DEAD/H-box RNA helicase binding
A	0031047	biological_process	regulatory ncRNA-mediated gene silencing
A	0031053	biological_process	primary miRNA processing
A	0031054	biological_process	pre-miRNA processing
A	0035196	biological_process	miRNA processing
A	0042254	biological_process	ribosome biogenesis
A	0042803	molecular_function	protein homodimerization activity
A	0043226	cellular_component	organelle
A	0045589	biological_process	regulation of regulatory T cell differentiation
A	0046332	molecular_function	SMAD binding
A	0046872	molecular_function	metal ion binding
A	0050727	biological_process	regulation of inflammatory response
A	0050829	biological_process	defense response to Gram-negative bacterium
A	0050830	biological_process	defense response to Gram-positive bacterium
A	0070412	molecular_function	R-SMAD binding
A	0070877	cellular_component	microprocessor complex
A	0070878	molecular_function	primary miRNA binding
A	0098978	cellular_component	glutamatergic synapse
A	2000628	biological_process	regulation of miRNA metabolic process
B	0003723	molecular_function	RNA binding
B	0003725	molecular_function	double-stranded RNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005730	cellular_component	nucleolus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006974	biological_process	DNA damage response
B	0014069	cellular_component	postsynaptic density
B	0016604	cellular_component	nuclear body
B	0020037	molecular_function	heme binding
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0031053	biological_process	primary miRNA processing
B	0035861	cellular_component	site of double-strand break
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0070877	cellular_component	microprocessor complex
B	0070878	molecular_function	primary miRNA binding
B	0072091	biological_process	regulation of stem cell proliferation
B	0098978	cellular_component	glutamatergic synapse
B	0140517	molecular_function	protein-RNA adaptor activity
B	2000633	biological_process	positive regulation of pre-miRNA processing
C	0003723	molecular_function	RNA binding
C	0003725	molecular_function	double-stranded RNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005730	cellular_component	nucleolus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006974	biological_process	DNA damage response
C	0014069	cellular_component	postsynaptic density
C	0016604	cellular_component	nuclear body
C	0020037	molecular_function	heme binding
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031053	biological_process	primary miRNA processing
C	0035861	cellular_component	site of double-strand break
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0070877	cellular_component	microprocessor complex
C	0070878	molecular_function	primary miRNA binding
C	0072091	biological_process	regulation of stem cell proliferation
C	0098978	cellular_component	glutamatergic synapse
C	0140517	molecular_function	protein-RNA adaptor activity
C	2000633	biological_process	positive regulation of pre-miRNA processing

Functional Information from PROSITE/UniProt

site_id	PS00517
Number of Residues	9
Details	RNASE_3_1 Ribonuclease III family signature. ERLEFLGDA

Chain	Residue	Details
A	GLU966-ALA974
A	GLN1144-SER1152

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	180
Details	Domain: {"description":"RNase III 1"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	126
Details	Domain: {"description":"RNase III 2"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	74
Details	Domain: {"description":"DRBM"}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"26748718","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"O67082","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"O67082","evidenceCode":"ECO:0000250"},{"source":"PubMed","id":"15574589","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	1
Details	Site: {"description":"Important for activity","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	67
Details	Domain: {"description":"DRBM 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00266","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	65
Details	Domain: {"description":"DRBM 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00266","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; by ATM","evidences":[{"source":"PubMed","id":"34188037","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25755297","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)