8Y2C

Cryo-EM structure of human dopamine transporter in apo state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001504	biological_process	neurotransmitter uptake
A	0002020	molecular_function	protease binding
A	0005102	molecular_function	signaling receptor binding
A	0005326	molecular_function	neurotransmitter transmembrane transporter activity
A	0005330	molecular_function	dopamine:sodium symporter activity
A	0005334	molecular_function	norepinephrine:sodium symporter activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005886	cellular_component	plasma membrane
A	0006836	biological_process	neurotransmitter transport
A	0006865	biological_process	amino acid transport
A	0008504	molecular_function	monoamine transmembrane transporter activity
A	0009410	biological_process	response to xenobiotic stimulus
A	0009986	cellular_component	cell surface
A	0010039	biological_process	response to iron ion
A	0015293	molecular_function	symporter activity
A	0015844	biological_process	monoamine transport
A	0015872	biological_process	dopamine transport
A	0016020	cellular_component	membrane
A	0016600	cellular_component	flotillin complex
A	0022857	molecular_function	transmembrane transporter activity
A	0030424	cellular_component	axon
A	0035094	biological_process	response to nicotine
A	0035240	molecular_function	dopamine binding
A	0035725	biological_process	sodium ion transmembrane transport
A	0042734	cellular_component	presynaptic membrane
A	0043005	cellular_component	neuron projection
A	0043025	cellular_component	neuronal cell body
A	0043176	molecular_function	amine binding
A	0043679	cellular_component	axon terminus
A	0044877	molecular_function	protein-containing complex binding
A	0045121	cellular_component	membrane raft
A	0045211	cellular_component	postsynaptic membrane
A	0045471	biological_process	response to ethanol
A	0046872	molecular_function	metal ion binding
A	0051583	biological_process	dopamine uptake involved in synaptic transmission
A	0051591	biological_process	response to cAMP
A	0051620	biological_process	norepinephrine uptake
A	0051721	molecular_function	protein phosphatase 2A binding
A	0090494	biological_process	dopamine uptake
A	0098691	cellular_component	dopaminergic synapse
A	1901363	molecular_function	heterocyclic compound binding
A	1990384	biological_process	hyaloid vascular plexus regression

Functional Information from PROSITE/UniProt

site_id	PS00610
Number of Residues	15
Details	NA_NEUROTRAN_SYMP_1 Sodium:neurotransmitter symporter family signature 1. WRFPYlcykNGGGaF

Chain	Residue	Details
A	TRP84-PHE98

---

site_id	PS00754
Number of Residues	21
Details	NA_NEUROTRAN_SYMP_2 Sodium:neurotransmitter symporter family signature 2. YLfsSFTteLPWihCnnswNS

Chain	Residue	Details
A	TYR166-SER186

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	31
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	43
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	19
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	51
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	28
Details	Transmembrane: {"description":"Discontinuously helical; Name=6","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	40
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	41
Details	Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	23
Details	Transmembrane: {"description":"Helical; Name=9","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	32
Details	Transmembrane: {"description":"Helical; Name=10","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	52
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	25
Details	Transmembrane: {"description":"Helical; Name=11","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	29
Details	Transmembrane: {"description":"Helical; Name=12","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	29
Details	Region: {"description":"Interaction with TGFB1I1","evidences":[{"source":"PubMed","id":"12177201","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112705","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VBY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39112703","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Y2D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"9EO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	1
Details	Site: {"description":"Contributes to high-affinity binding to cocaine","evidences":[{"source":"UniProtKB","id":"Q61327","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"39112701","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Y2C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Y2G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---