8XIM
PROTEIN ENGINEERING OF XYLOSE (GLUCOSE) ISOMERASE FROM ACTINOPLANES MISSOURIENSIS. 1. CRYSTALLOGRAPHY AND SITE-DIRECTED MUTAGENESIS OF METAL BINDING SITES
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0009045 | molecular_function | xylose isomerase activity |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0042732 | biological_process | D-xylose metabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0009045 | molecular_function | xylose isomerase activity |
| B | 0016853 | molecular_function | isomerase activity |
| B | 0042732 | biological_process | D-xylose metabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0009045 | molecular_function | xylose isomerase activity |
| C | 0016853 | molecular_function | isomerase activity |
| C | 0042732 | biological_process | D-xylose metabolic process |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0009045 | molecular_function | xylose isomerase activity |
| D | 0016853 | molecular_function | isomerase activity |
| D | 0042732 | biological_process | D-xylose metabolic process |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: |
| Chain | Residue | Details |
| A | ASP55 | |
| B | ASP55 | |
| C | ASP55 | |
| D | ASP55 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: ACT_SITE => ECO:0000250 |
| Chain | Residue | Details |
| A | LEU58 | |
| B | LEU58 | |
| C | LEU58 | |
| D | LEU58 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 28 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | PRO182 | |
| B | GLU221 | |
| B | LEU246 | |
| B | GLN256 | |
| B | LEU258 | |
| B | TYR293 | |
| C | PRO182 | |
| C | THR218 | |
| C | GLU221 | |
| C | LEU246 | |
| C | GLN256 | |
| A | THR218 | |
| C | LEU258 | |
| C | TYR293 | |
| D | PRO182 | |
| D | THR218 | |
| D | GLU221 | |
| D | LEU246 | |
| D | GLN256 | |
| D | LEU258 | |
| D | TYR293 | |
| A | GLU221 | |
| A | LEU246 | |
| A | GLN256 | |
| A | LEU258 | |
| A | TYR293 | |
| B | PRO182 | |
| B | THR218 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| A | LYS183 | |
| A | HIS220 | |
| A | ASP255 |
| site_id | CSA10 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| B | GLU181 | |
| B | HIS54 | |
| B | LYS183 | |
| B | ASP57 |
| site_id | CSA11 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| C | GLU181 | |
| C | HIS54 | |
| C | LYS183 | |
| C | ASP57 |
| site_id | CSA12 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| D | GLU181 | |
| D | HIS54 | |
| D | LYS183 | |
| D | ASP57 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| B | LYS183 | |
| B | HIS220 | |
| B | ASP255 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| C | LYS183 | |
| C | HIS220 | |
| C | ASP255 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| D | LYS183 | |
| D | HIS220 | |
| D | ASP255 |
| site_id | CSA5 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| A | ARG297 |
| site_id | CSA6 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| B | ARG297 |
| site_id | CSA7 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| C | ARG297 |
| site_id | CSA8 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| D | ARG297 |
| site_id | CSA9 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1de6 |
| Chain | Residue | Details |
| A | GLU181 | |
| A | HIS54 | |
| A | LYS183 | |
| A | ASP57 |
