8X06
Cryo-EM structure of the IR/IGF-I complex, conformation 1
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004713 | molecular_function | protein tyrosine kinase activity |
| A | 0004714 | molecular_function | transmembrane receptor protein tyrosine kinase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0007169 | biological_process | cell surface receptor protein tyrosine kinase signaling pathway |
| A | 0016020 | cellular_component | membrane |
| A | 0043548 | molecular_function | phosphatidylinositol 3-kinase binding |
| A | 0043560 | molecular_function | insulin receptor substrate binding |
| A | 0046777 | biological_process | protein autophosphorylation |
| B | 0004672 | molecular_function | protein kinase activity |
| B | 0004713 | molecular_function | protein tyrosine kinase activity |
| B | 0004714 | molecular_function | transmembrane receptor protein tyrosine kinase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006468 | biological_process | protein phosphorylation |
| B | 0007169 | biological_process | cell surface receptor protein tyrosine kinase signaling pathway |
| B | 0016020 | cellular_component | membrane |
| B | 0043548 | molecular_function | phosphatidylinositol 3-kinase binding |
| B | 0043560 | molecular_function | insulin receptor substrate binding |
| B | 0046777 | biological_process | protein autophosphorylation |
| C | 0005179 | molecular_function | hormone activity |
| C | 0005576 | cellular_component | extracellular region |
| C | 0005615 | cellular_component | extracellular space |
| C | 0008083 | molecular_function | growth factor activity |
| D | 0005179 | molecular_function | hormone activity |
| D | 0005576 | cellular_component | extracellular region |
| D | 0005615 | cellular_component | extracellular space |
| D | 0008083 | molecular_function | growth factor activity |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 29 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGQGSFGMVYeGnardiikgeaetr.....VAVK |
| Chain | Residue | Details |
| A | LEU990-LYS1018 |
| site_id | PS00109 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_TYR Tyrosine protein kinases specific active-site signature. FVHrDLAARNCMV |
| Chain | Residue | Details |
| A | PHE1116-VAL1128 |
| site_id | PS00239 |
| Number of Residues | 9 |
| Details | RECEPTOR_TYR_KIN_II Receptor tyrosine kinase class II signature. DIYetdYYR |
| Chain | Residue | Details |
| A | ASP1144-ARG1152 |
| site_id | PS00262 |
| Number of Residues | 15 |
| Details | INSULIN Insulin family signature. CCFRsCDlrrLemyC |
| Chain | Residue | Details |
| C | CYS47-CYS61 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1846 |
| Details | TOPO_DOM: Extracellular => ECO:0000305 |
| Chain | Residue | Details |
| A | HIS1-VAL731 | |
| A | PRO736-PHE929 | |
| B | HIS191-VAL921 | |
| B | PRO926-PHE1119 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical => ECO:0000255 |
| Chain | Residue | Details |
| A | SER930-LEU952 | |
| B | SER1120-LEU1142 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000269|PubMed:9312016 |
| Chain | Residue | Details |
| A | PHE1132 | |
| B | PHE1322 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000269|PubMed:18278056 |
| Chain | Residue | Details |
| A | ILE1006 | |
| B | TYR1340 | |
| A | ILE1030 | |
| A | ARG1077 | |
| A | ILE1136 | |
| A | TYR1150 | |
| B | ILE1196 | |
| B | ILE1220 | |
| B | ARG1267 | |
| B | ILE1326 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | SITE: Insulin-binding => ECO:0000305 |
| Chain | Residue | Details |
| A | PHE39 | |
| B | PHE229 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| A | SER373 | |
| A | SER380 | |
| B | SER563 | |
| B | SER570 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| A | TYR374 | |
| B | TYR564 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000305 |
| Chain | Residue | Details |
| A | ASP965 | |
| A | ILE984 | |
| B | ASP1155 | |
| B | ILE1174 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000305|PubMed:3166375 |
| Chain | Residue | Details |
| A | TYR972 | |
| A | GLY1328 | |
| A | ILE1334 | |
| B | TYR1162 | |
| B | GLY1518 | |
| B | ILE1524 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | MOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:38056462 |
| Chain | Residue | Details |
| A | LYS1056 | |
| B | LYS1246 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:14690593, ECO:0000269|PubMed:16246733, ECO:0000269|PubMed:16271887, ECO:0000269|PubMed:18278056, ECO:0000269|PubMed:18767165, ECO:0000269|PubMed:26584640, ECO:0000269|PubMed:3166375, ECO:0000269|PubMed:9312016 |
| Chain | Residue | Details |
| A | LEU1158 | |
| A | TRP1163 | |
| B | LEU1348 | |
| B | TRP1353 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:14690593, ECO:0000269|PubMed:16246733, ECO:0000269|PubMed:16271887, ECO:0000269|PubMed:18278056, ECO:0000269|PubMed:18767165, ECO:0000269|PubMed:3166375, ECO:0000269|PubMed:9312016 |
| Chain | Residue | Details |
| A | ARG1162 | |
| B | ARG1352 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147, ECO:0000269|PubMed:23302862, ECO:0000269|PubMed:2983222 |
| Chain | Residue | Details |
| A | ASN16 | |
| B | ASN206 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 6 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147, ECO:0000269|PubMed:23302862 |
| Chain | Residue | Details |
| A | ASN25 | |
| A | ASN111 | |
| A | ASN255 | |
| B | ASN215 | |
| B | ASN301 | |
| B | ASN445 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 14 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| A | ASN78 | |
| B | ASN796 | |
| B | ASN814 | |
| B | ASN861 | |
| B | GLU945 | |
| B | THR1096 | |
| A | ASN295 | |
| A | ASN606 | |
| A | ASN624 | |
| A | ASN671 | |
| A | GLU755 | |
| A | THR906 | |
| B | ASN268 | |
| B | ASN485 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:23302862 |
| Chain | Residue | Details |
| A | ASN215 | |
| B | ASN405 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147 |
| Chain | Residue | Details |
| A | ASN337 | |
| A | ASN397 | |
| B | ASN527 | |
| B | ASN587 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147, ECO:0000269|PubMed:19349973 |
| Chain | Residue | Details |
| A | ASN418 | |
| B | ASN608 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:1472036, ECO:0000269|PubMed:19159218 |
| Chain | Residue | Details |
| A | ASN514 | |
| B | ASN704 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:2983222 |
| Chain | Residue | Details |
| A | PRO742 | |
| B | PRO932 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19349973 |
| Chain | Residue | Details |
| A | GLY893 | |
| B | GLY1083 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:27577745 |
| Chain | Residue | Details |
| A | GLY1052 | |
| B | GLY1242 |
