Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8WQH

cryo-EM structure of neddylated CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CCDC89 (conformation 2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000082	biological_process	G1/S transition of mitotic cell cycle
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005829	cellular_component	cytosol
A	0016567	biological_process	protein ubiquitination
A	0019005	cellular_component	SCF ubiquitin ligase complex
A	0030163	biological_process	protein catabolic process
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0030891	cellular_component	VCB complex
A	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
A	0031461	cellular_component	cullin-RING ubiquitin ligase complex
A	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0044877	molecular_function	protein-containing complex binding
A	0097193	biological_process	intrinsic apoptotic signaling pathway
A	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
A	0160072	molecular_function	ubiquitin ligase complex scaffold activity
C	0000082	biological_process	G1/S transition of mitotic cell cycle
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005730	cellular_component	nucleolus
C	0005829	cellular_component	cytosol
C	0016567	biological_process	protein ubiquitination
C	0019005	cellular_component	SCF ubiquitin ligase complex
C	0030163	biological_process	protein catabolic process
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0030891	cellular_component	VCB complex
C	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
C	0031461	cellular_component	cullin-RING ubiquitin ligase complex
C	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0044877	molecular_function	protein-containing complex binding
C	0097193	biological_process	intrinsic apoptotic signaling pathway
C	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
C	0160072	molecular_function	ubiquitin ligase complex scaffold activity
D	0002070	biological_process	epithelial cell maturation
D	0005123	molecular_function	death receptor binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006915	biological_process	apoptotic process
D	0016567	biological_process	protein ubiquitination
D	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0046872	molecular_function	metal ion binding
D	0051438	biological_process	regulation of ubiquitin-protein transferase activity
D	0060442	biological_process	branching involved in prostate gland morphogenesis
D	0060743	biological_process	epithelial cell maturation involved in prostate gland development
D	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
D	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
D	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
D	2000001	biological_process	regulation of DNA damage checkpoint
F	0001222	molecular_function	transcription corepressor binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005829	cellular_component	cytosol
F	0006367	biological_process	transcription initiation at RNA polymerase II promoter
F	0006368	biological_process	transcription elongation by RNA polymerase II
F	0016567	biological_process	protein ubiquitination
F	0030891	cellular_component	VCB complex
F	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
F	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
F	0065003	biological_process	protein-containing complex assembly
F	0070449	cellular_component	elongin complex
F	0140958	biological_process	target-directed miRNA degradation
H	0002070	biological_process	epithelial cell maturation
H	0005123	molecular_function	death receptor binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006915	biological_process	apoptotic process
H	0016567	biological_process	protein ubiquitination
H	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
H	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
H	0046872	molecular_function	metal ion binding
H	0051438	biological_process	regulation of ubiquitin-protein transferase activity
H	0060442	biological_process	branching involved in prostate gland morphogenesis
H	0060743	biological_process	epithelial cell maturation involved in prostate gland development
H	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
H	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
H	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
H	2000001	biological_process	regulation of DNA damage checkpoint
K	0001222	molecular_function	transcription corepressor binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005829	cellular_component	cytosol
K	0006367	biological_process	transcription initiation at RNA polymerase II promoter
K	0006368	biological_process	transcription elongation by RNA polymerase II
K	0016567	biological_process	protein ubiquitination
K	0030891	cellular_component	VCB complex
K	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
K	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
K	0031625	molecular_function	ubiquitin protein ligase binding
K	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
K	0065003	biological_process	protein-containing complex assembly
K	0070449	cellular_component	elongin complex
K	0140958	biological_process	target-directed miRNA degradation

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
C	ILE718-ALA745

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:Q9Z2G0

Chain	Residue	Details
D	HIS185
D	CYS186
D	HIS218
H	HIS185
H	CYS186
H	HIS218

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Cleavage; by a caspase-3-like protease => ECO:0000269\|PubMed:10542291

Chain	Residue	Details
D	ASP342
J	CYS94
J	ASP97
B	CYS42
B	CYS45
B	CYS53
B	CYS56
B	CYS68
B	CYS75
B	HIS77
B	HIS80
H	ASP342
B	HIS82
B	CYS94
B	ASP97
J	CYS53
J	CYS56
J	CYS68
J	CYS75
J	HIS77
J	HIS80
J	HIS82

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P62869

Chain	Residue	Details
F	SER108
F	SER111
K	SER108
K	SER111

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)