Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8WQC

cryo-EM structure of neddylated CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CDK5R1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002070	biological_process	epithelial cell maturation
A	0005123	molecular_function	death receptor binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006915	biological_process	apoptotic process
A	0016567	biological_process	protein ubiquitination
A	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0046872	molecular_function	metal ion binding
A	0051438	biological_process	regulation of ubiquitin-protein transferase activity
A	0060442	biological_process	branching involved in prostate gland morphogenesis
A	0060743	biological_process	epithelial cell maturation involved in prostate gland development
A	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
A	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
A	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
A	2000001	biological_process	regulation of DNA damage checkpoint
C	0000082	biological_process	G1/S transition of mitotic cell cycle
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005730	cellular_component	nucleolus
C	0005829	cellular_component	cytosol
C	0016567	biological_process	protein ubiquitination
C	0019005	cellular_component	SCF ubiquitin ligase complex
C	0030163	biological_process	protein catabolic process
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0030891	cellular_component	VCB complex
C	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
C	0031461	cellular_component	cullin-RING ubiquitin ligase complex
C	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0044877	molecular_function	protein-containing complex binding
C	0097193	biological_process	intrinsic apoptotic signaling pathway
C	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
C	0160072	molecular_function	ubiquitin ligase complex scaffold activity
D	0001222	molecular_function	transcription corepressor binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005829	cellular_component	cytosol
D	0006367	biological_process	transcription initiation at RNA polymerase II promoter
D	0006368	biological_process	transcription elongation by RNA polymerase II
D	0016567	biological_process	protein ubiquitination
D	0030891	cellular_component	VCB complex
D	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
D	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
D	0065003	biological_process	protein-containing complex assembly
D	0070449	cellular_component	elongin complex
D	0140958	biological_process	target-directed miRNA degradation
E	0000082	biological_process	G1/S transition of mitotic cell cycle
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005730	cellular_component	nucleolus
E	0005829	cellular_component	cytosol
E	0016567	biological_process	protein ubiquitination
E	0019005	cellular_component	SCF ubiquitin ligase complex
E	0030163	biological_process	protein catabolic process
E	0030674	molecular_function	protein-macromolecule adaptor activity
E	0030891	cellular_component	VCB complex
E	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
E	0031461	cellular_component	cullin-RING ubiquitin ligase complex
E	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0044877	molecular_function	protein-containing complex binding
E	0097193	biological_process	intrinsic apoptotic signaling pathway
E	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
E	0160072	molecular_function	ubiquitin ligase complex scaffold activity
G	0002070	biological_process	epithelial cell maturation
G	0005123	molecular_function	death receptor binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006915	biological_process	apoptotic process
G	0016567	biological_process	protein ubiquitination
G	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0046872	molecular_function	metal ion binding
G	0051438	biological_process	regulation of ubiquitin-protein transferase activity
G	0060442	biological_process	branching involved in prostate gland morphogenesis
G	0060743	biological_process	epithelial cell maturation involved in prostate gland development
G	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
G	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
G	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
G	2000001	biological_process	regulation of DNA damage checkpoint
I	0001222	molecular_function	transcription corepressor binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005829	cellular_component	cytosol
I	0006367	biological_process	transcription initiation at RNA polymerase II promoter
I	0006368	biological_process	transcription elongation by RNA polymerase II
I	0016567	biological_process	protein ubiquitination
I	0030891	cellular_component	VCB complex
I	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
I	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
I	0031625	molecular_function	ubiquitin protein ligase binding
I	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
I	0065003	biological_process	protein-containing complex assembly
I	0070449	cellular_component	elongin complex
I	0140958	biological_process	target-directed miRNA degradation
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005829	cellular_component	cytosol
K	0006357	biological_process	regulation of transcription by RNA polymerase II
K	0006508	biological_process	proteolysis
K	0006511	biological_process	ubiquitin-dependent protein catabolic process
K	0008104	biological_process	protein localization
K	0009653	biological_process	anatomical structure morphogenesis
K	0016567	biological_process	protein ubiquitination
K	0019941	biological_process	modification-dependent protein catabolic process
K	0030162	biological_process	regulation of proteolysis
K	0031386	molecular_function	protein tag activity
K	0031625	molecular_function	ubiquitin protein ligase binding
K	0036211	biological_process	protein modification process
K	0045116	biological_process	protein neddylation
K	0070062	cellular_component	extracellular exosome
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005829	cellular_component	cytosol
N	0006357	biological_process	regulation of transcription by RNA polymerase II
N	0006508	biological_process	proteolysis
N	0006511	biological_process	ubiquitin-dependent protein catabolic process
N	0008104	biological_process	protein localization
N	0009653	biological_process	anatomical structure morphogenesis
N	0016567	biological_process	protein ubiquitination
N	0019941	biological_process	modification-dependent protein catabolic process
N	0030162	biological_process	regulation of proteolysis
N	0031386	molecular_function	protein tag activity
N	0031625	molecular_function	ubiquitin protein ligase binding
N	0036211	biological_process	protein modification process
N	0045116	biological_process	protein neddylation
N	0070062	cellular_component	extracellular exosome

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KerVeEkegIPpqqQrLIYsGkqmnD

Chain	Residue	Details
N	LYS27-ASP52

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
C	ILE718-ALA745

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:Q9Z2G0

Chain	Residue	Details
A	HIS185
A	CYS186
A	HIS218
G	HIS185
G	CYS186
G	HIS218

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Cleavage; by a caspase-3-like protease => ECO:0000269\|PubMed:10542291

Chain	Residue	Details
A	ASP342
F	CYS94
F	ASP97
J	CYS42
J	CYS45
J	CYS53
J	CYS56
J	CYS68
J	CYS75
J	HIS77
J	HIS80
G	ASP342
J	HIS82
J	CYS94
J	ASP97
F	CYS53
F	CYS56
F	CYS68
F	CYS75
F	HIS77
F	HIS80
F	HIS82

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:P29595

Chain	Residue	Details
N	LYS48
K	LYS48
E	ILE704
I	SER111

site_id	SWS_FT_FI4
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
N	GLY76
K	GLY76

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)