Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8WQB

Cryo-EM structure of CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CCDC89 (conformation 2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000082	biological_process	G1/S transition of mitotic cell cycle
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005829	cellular_component	cytosol
A	0016567	biological_process	protein ubiquitination
A	0019005	cellular_component	SCF ubiquitin ligase complex
A	0030163	biological_process	protein catabolic process
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0030891	cellular_component	VCB complex
A	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
A	0031461	cellular_component	cullin-RING ubiquitin ligase complex
A	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0044877	molecular_function	protein-containing complex binding
A	0097193	biological_process	intrinsic apoptotic signaling pathway
A	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
A	0160072	molecular_function	ubiquitin ligase complex scaffold activity
B	0000082	biological_process	G1/S transition of mitotic cell cycle
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005730	cellular_component	nucleolus
B	0005829	cellular_component	cytosol
B	0016567	biological_process	protein ubiquitination
B	0019005	cellular_component	SCF ubiquitin ligase complex
B	0030163	biological_process	protein catabolic process
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0030891	cellular_component	VCB complex
B	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
B	0031461	cellular_component	cullin-RING ubiquitin ligase complex
B	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0044877	molecular_function	protein-containing complex binding
B	0097193	biological_process	intrinsic apoptotic signaling pathway
B	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
B	0160072	molecular_function	ubiquitin ligase complex scaffold activity
D	0001222	molecular_function	transcription corepressor binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005829	cellular_component	cytosol
D	0006367	biological_process	transcription initiation at RNA polymerase II promoter
D	0006368	biological_process	transcription elongation by RNA polymerase II
D	0016567	biological_process	protein ubiquitination
D	0030891	cellular_component	VCB complex
D	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
D	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
D	0065003	biological_process	protein-containing complex assembly
D	0070449	cellular_component	elongin complex
D	0140958	biological_process	target-directed miRNA degradation
F	0002070	biological_process	epithelial cell maturation
F	0005123	molecular_function	death receptor binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006915	biological_process	apoptotic process
F	0016567	biological_process	protein ubiquitination
F	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0046872	molecular_function	metal ion binding
F	0051438	biological_process	regulation of ubiquitin-protein transferase activity
F	0060442	biological_process	branching involved in prostate gland morphogenesis
F	0060743	biological_process	epithelial cell maturation involved in prostate gland development
F	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
F	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
F	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
F	2000001	biological_process	regulation of DNA damage checkpoint
H	0001222	molecular_function	transcription corepressor binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005829	cellular_component	cytosol
H	0006367	biological_process	transcription initiation at RNA polymerase II promoter
H	0006368	biological_process	transcription elongation by RNA polymerase II
H	0016567	biological_process	protein ubiquitination
H	0030891	cellular_component	VCB complex
H	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
H	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
H	0031625	molecular_function	ubiquitin protein ligase binding
H	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
H	0065003	biological_process	protein-containing complex assembly
H	0070449	cellular_component	elongin complex
H	0140958	biological_process	target-directed miRNA degradation
J	0002070	biological_process	epithelial cell maturation
J	0005123	molecular_function	death receptor binding
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006915	biological_process	apoptotic process
J	0016567	biological_process	protein ubiquitination
J	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0046872	molecular_function	metal ion binding
J	0051438	biological_process	regulation of ubiquitin-protein transferase activity
J	0060442	biological_process	branching involved in prostate gland morphogenesis
J	0060743	biological_process	epithelial cell maturation involved in prostate gland development
J	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
J	1902041	biological_process	regulation of extrinsic apoptotic signaling pathway via death domain receptors
J	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
J	2000001	biological_process	regulation of DNA damage checkpoint

Functional Information from PROSITE/UniProt

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIevLIDKqYIeRsqasadeYsYvA

Chain	Residue	Details
A	ILE718-ALA745

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:Q9Z2G0

Chain	Residue	Details
J	HIS185
J	CYS186
J	HIS218
F	HIS185
F	CYS186
F	HIS218

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Cleavage; by a caspase-3-like protease => ECO:0000269\|PubMed:10542291

Chain	Residue	Details
J	ASP342
F	ASP342
I	CYS53
I	CYS56
I	CYS68
I	CYS75
I	HIS77
I	HIS80
I	HIS82
I	CYS94
I	ASP97
E	CYS42
E	CYS45
E	CYS53
E	CYS56
E	CYS68
E	CYS75
E	HIS77
E	HIS80
E	HIS82
E	CYS94
E	ASP97

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P62869

Chain	Residue	Details
D	SER108
D	SER111
H	SER108
H	SER111

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)