8WM3
Cryo-EM structure of ACE2-SIT1 complex with tiagabine
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005298 | molecular_function | proline:sodium symporter activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006865 | biological_process | amino acid transport |
| A | 0015171 | molecular_function | amino acid transmembrane transporter activity |
| A | 0015175 | molecular_function | neutral L-amino acid transmembrane transporter activity |
| A | 0015188 | molecular_function | L-isoleucine transmembrane transporter activity |
| A | 0015193 | molecular_function | L-proline transmembrane transporter activity |
| A | 0015199 | molecular_function | amino-acid betaine transmembrane transporter activity |
| A | 0015293 | molecular_function | symporter activity |
| A | 0015370 | molecular_function | solute:sodium symporter activity |
| A | 0015816 | biological_process | glycine transport |
| A | 0015824 | biological_process | proline transport |
| A | 0015838 | biological_process | amino-acid betaine transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016324 | cellular_component | apical plasma membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0035725 | biological_process | sodium ion transmembrane transport |
| A | 0089718 | biological_process | amino acid import across plasma membrane |
| A | 0150104 | biological_process | transport across blood-brain barrier |
| A | 1903804 | biological_process | glycine import across plasma membrane |
| A | 1903806 | biological_process | L-isoleucine import across plasma membrane |
| A | 1904271 | biological_process | L-proline import across plasma membrane |
| A | 1905647 | biological_process | proline import across plasma membrane |
| B | 0006508 | biological_process | proteolysis |
| B | 0008237 | molecular_function | metallopeptidase activity |
| B | 0008241 | molecular_function | peptidyl-dipeptidase activity |
| B | 0016020 | cellular_component | membrane |
| C | 0005298 | molecular_function | proline:sodium symporter activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006865 | biological_process | amino acid transport |
| C | 0015171 | molecular_function | amino acid transmembrane transporter activity |
| C | 0015175 | molecular_function | neutral L-amino acid transmembrane transporter activity |
| C | 0015188 | molecular_function | L-isoleucine transmembrane transporter activity |
| C | 0015193 | molecular_function | L-proline transmembrane transporter activity |
| C | 0015199 | molecular_function | amino-acid betaine transmembrane transporter activity |
| C | 0015293 | molecular_function | symporter activity |
| C | 0015370 | molecular_function | solute:sodium symporter activity |
| C | 0015816 | biological_process | glycine transport |
| C | 0015824 | biological_process | proline transport |
| C | 0015838 | biological_process | amino-acid betaine transport |
| C | 0016020 | cellular_component | membrane |
| C | 0016324 | cellular_component | apical plasma membrane |
| C | 0022857 | molecular_function | transmembrane transporter activity |
| C | 0035725 | biological_process | sodium ion transmembrane transport |
| C | 0089718 | biological_process | amino acid import across plasma membrane |
| C | 0150104 | biological_process | transport across blood-brain barrier |
| C | 1903804 | biological_process | glycine import across plasma membrane |
| C | 1903806 | biological_process | L-isoleucine import across plasma membrane |
| C | 1904271 | biological_process | L-proline import across plasma membrane |
| C | 1905647 | biological_process | proline import across plasma membrane |
| D | 0006508 | biological_process | proteolysis |
| D | 0008237 | molecular_function | metallopeptidase activity |
| D | 0008241 | molecular_function | peptidyl-dipeptidase activity |
| D | 0016020 | cellular_component | membrane |
Functional Information from PROSITE/UniProt
| site_id | PS00142 |
| Number of Residues | 10 |
| Details | ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. TAHHEMGHIQ |
| Chain | Residue | Details |
| B | THR371-GLN380 |
| site_id | PS00610 |
| Number of Residues | 15 |
| Details | NA_NEUROTRAN_SYMP_1 Sodium:neurotransmitter symporter family signature 1. WRFPYlcqmYGGGsF |
| Chain | Residue | Details |
| A | TRP29-PHE43 |
| site_id | PS00754 |
| Number of Residues | 21 |
| Details | NA_NEUROTRAN_SYMP_2 Sodium:neurotransmitter symporter family signature 2. YLfhSFQdpLPWsvCplngNH |
| Chain | Residue | Details |
| A | TYR112-HIS132 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1444 |
| Details | TOPO_DOM: Extracellular => ECO:0000255 |
| Chain | Residue | Details |
| B | GLN18-SER740 | |
| C | ARG187-LYS194 | |
| C | ALA263-ALA276 | |
| C | THR411-GLU431 | |
| C | ARG487-TYR504 | |
| C | GLY576-ALA592 | |
| D | GLN18-SER740 | |
| A | ARG187-LYS194 | |
| A | ALA263-ALA276 | |
| A | THR411-GLU431 | |
| A | ARG487-TYR504 | |
| A | GLY576-ALA592 | |
| C | MET1-ARG5 | |
| C | ALA64-THR79 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 40 |
| Details | TRANSMEM: Helical => ECO:0000255|PROSITE-ProRule:PRU01354 |
| Chain | Residue | Details |
| B | ILE741-ILE761 | |
| D | ILE741-ILE761 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 86 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000255 |
| Chain | Residue | Details |
| B | PHE762-PHE805 | |
| C | TYR298-GLN389 | |
| C | GLY453-ALA465 | |
| C | SER526-TYR554 | |
| D | PHE762-PHE805 | |
| A | THR216-TRP241 | |
| A | TYR298-GLN389 | |
| A | GLY453-ALA465 | |
| A | SER526-TYR554 | |
| C | ASN27-SER42 | |
| C | TYR101-GLY165 | |
| C | THR216-TRP241 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000305|PubMed:14754895, ECO:0000305|PubMed:27217402 |
| Chain | Residue | Details |
| B | GLU375 | |
| D | GLU375 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000305|PubMed:14754895 |
| Chain | Residue | Details |
| B | HIS505 | |
| D | HIS505 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:14754895, ECO:0000305|PubMed:19021774 |
| Chain | Residue | Details |
| B | ARG169 | |
| B | TRP477 | |
| B | LYS481 | |
| D | ARG169 | |
| D | TRP477 | |
| D | LYS481 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:14754895 |
| Chain | Residue | Details |
| B | ARG273 | |
| B | HIS345 | |
| B | TYR515 | |
| D | ARG273 | |
| D | HIS345 | |
| D | TYR515 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:14754895 |
| Chain | Residue | Details |
| B | HIS374 | |
| B | HIS378 | |
| B | GLU402 | |
| D | HIS374 | |
| D | HIS378 | |
| D | GLU402 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000305|PubMed:33436497, ECO:0000305|PubMed:33436498 |
| Chain | Residue | Details |
| B | TYR781 | |
| D | TYR781 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000305|PubMed:33436497, ECO:0000305|PubMed:33436498 |
| Chain | Residue | Details |
| B | SER783 | |
| D | SER783 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000305|PubMed:14754895 |
| Chain | Residue | Details |
| B | ASN53 | |
| B | ASN322 | |
| D | ASN53 | |
| D | ASN322 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:14754895, ECO:0000269|PubMed:15084671, ECO:0000269|PubMed:19901337 |
| Chain | Residue | Details |
| B | ASN90 | |
| D | ASN90 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:14754895 |
| Chain | Residue | Details |
| B | ASN103 | |
| B | ASN432 | |
| D | ASN103 | |
| D | ASN432 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:14754895, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:19901337 |
| Chain | Residue | Details |
| B | ASN546 | |
| D | ASN546 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| B | ASN690 | |
| D | ASN690 | |
| C | ASN131 | |
| C | ASN357 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:36876523 |
| Chain | Residue | Details |
| B | LYS788 | |
| D | LYS788 |
