8WKL

Rauvolfia serpentina strictosidine synthase (RsSTR) in complex with a non-reactive tryptamine substitute crystallized in P1211 space group

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005773	cellular_component	vacuole
A	0009820	biological_process	alkaloid metabolic process
A	0009821	biological_process	alkaloid biosynthetic process
A	0012505	cellular_component	endomembrane system
A	0016787	molecular_function	hydrolase activity
A	0016844	molecular_function	strictosidine synthase activity
A	0035834	biological_process	indole alkaloid metabolic process
B	0005773	cellular_component	vacuole
B	0009820	biological_process	alkaloid metabolic process
B	0009821	biological_process	alkaloid biosynthetic process
B	0012505	cellular_component	endomembrane system
B	0016787	molecular_function	hydrolase activity
B	0016844	molecular_function	strictosidine synthase activity
B	0035834	biological_process	indole alkaloid metabolic process
C	0005773	cellular_component	vacuole
C	0009820	biological_process	alkaloid metabolic process
C	0009821	biological_process	alkaloid biosynthetic process
C	0012505	cellular_component	endomembrane system
C	0016787	molecular_function	hydrolase activity
C	0016844	molecular_function	strictosidine synthase activity
C	0035834	biological_process	indole alkaloid metabolic process
D	0005773	cellular_component	vacuole
D	0009820	biological_process	alkaloid metabolic process
D	0009821	biological_process	alkaloid biosynthetic process
D	0012505	cellular_component	endomembrane system
D	0016787	molecular_function	hydrolase activity
D	0016844	molecular_function	strictosidine synthase activity
D	0035834	biological_process	indole alkaloid metabolic process
E	0005773	cellular_component	vacuole
E	0009820	biological_process	alkaloid metabolic process
E	0009821	biological_process	alkaloid biosynthetic process
E	0012505	cellular_component	endomembrane system
E	0016787	molecular_function	hydrolase activity
E	0016844	molecular_function	strictosidine synthase activity
E	0035834	biological_process	indole alkaloid metabolic process
F	0005773	cellular_component	vacuole
F	0009820	biological_process	alkaloid metabolic process
F	0009821	biological_process	alkaloid biosynthetic process
F	0012505	cellular_component	endomembrane system
F	0016787	molecular_function	hydrolase activity
F	0016844	molecular_function	strictosidine synthase activity
F	0035834	biological_process	indole alkaloid metabolic process
G	0005773	cellular_component	vacuole
G	0009820	biological_process	alkaloid metabolic process
G	0009821	biological_process	alkaloid biosynthetic process
G	0012505	cellular_component	endomembrane system
G	0016787	molecular_function	hydrolase activity
G	0016844	molecular_function	strictosidine synthase activity
G	0035834	biological_process	indole alkaloid metabolic process
H	0005773	cellular_component	vacuole
H	0009820	biological_process	alkaloid metabolic process
H	0009821	biological_process	alkaloid biosynthetic process
H	0012505	cellular_component	endomembrane system
H	0016787	molecular_function	hydrolase activity
H	0016844	molecular_function	strictosidine synthase activity
H	0035834	biological_process	indole alkaloid metabolic process

Functional Information from PROSITE/UniProt

site_id	PS00284
Number of Residues	11
Details	SERPIN Serpins signature. VSADSSFVLvA

Chain	Residue	Details
A	VAL214-ALA224

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PubMed","id":"16531499","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18081287","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16531499","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2FPB","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16531499","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2FPC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00498","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
A	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---

site_id	MCSA2
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
B	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---

site_id	MCSA3
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
C	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---

site_id	MCSA4
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
D	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---

site_id	MCSA5
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
E	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---

site_id	MCSA6
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
F	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---

site_id	MCSA7
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
G	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---

site_id	MCSA8
Number of Residues	1
Details	M-CSA 360

Chain	Residue	Details
H	GLU309	activator, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity, proton acceptor, proton donor

---